NMDAR2A ELISA Kit

Produktnummer ABIN5706916

Produktdetails NMDAR2A ELISA Kit

Target: NMDAR2A (GRIN2A) (Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2a (GRIN2A))

Reaktivität: Ratte

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.156 ng/mL - 10 ng/mL

Untere Nachweisgrenze: 0.156 ng/mL

Applikation: ELISA

Proben: Cell Lysate, Tissue Homogenate

Analytische Methode: Quantitative

Sensitivität: 0.069 ng/mL

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

Pre-coated, ready to use 96-well strip plate,
Standard (freeze dried),
Standard Diluent,
Detection Reagent A,
Detection Reagent B,
Assay Diluent A,
Assay Diluent B,
TMB substrate,
Stop Solution,
Wash Buffer(30 x concenthumane),
Plate sealer for 96 wells,
Instruction manual

Probenmenge: 100 µL

Testdauer: 1 - 4.5 h

Plattentyp: Pre-coated

Protokoll: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8. Add 50µL Stop Solution. Read at 450nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Haltbarkeit: 12 months

Details zu NMDAR2A

Target: NMDAR2A (GRIN2A) (Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2a (GRIN2A))

Andere Bezeichnung: Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate 2A (GRIN2A) (GRIN2A Produkte)

Synonyme: NMDAR2A ELISA Kit, nr2a ELISA Kit, GRIN2A ELISA Kit, EPND ELISA Kit, GluN2A ELISA Kit, NR2A ELISA Kit, glutamate ionotropic receptor NMDA type subunit 2A ELISA Kit, glutamate receptor, ionotropic, N-methyl D-aspartate 2A L homeolog ELISA Kit, glutamate receptor ionotropic, NMDA 2A ELISA Kit, glutamate receptor, ionotropic, NMDA2A (epsilon 1) ELISA Kit, GRIN2A ELISA Kit, grin2a.L ELISA Kit, LOC100438553 ELISA Kit, Grin2a ELISA Kit

Hintergrund: Alternative name: NMDAR2A, NR2A, Glutamate [NMDA] receptor subunit epsilon-1, N-methyl D-aspartate receptor subtype 2A

Pathways: Synaptic Membrane, Regulation of long-term Neuronal Synaptic Plasticity