Estrogen Receptor alpha ELISA Kit

Produktnummer ABIN6244373

Produktdetails Estrogen Receptor alpha ELISA Kit

Target: Estrogen Receptor alpha (ESR1) (Estrogen Receptor 1 (ESR1))

Bindungsspezifität: pSer118, total

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Human Phospho-Estrogen receptor (Ser118) and Total Estrogen receptor ELISA Kit. This assay semi-quantitatively measures Estrogen receptor phosphorylated at Serine-118 as well as total Estrogen receptor in cell lysate samples.

Proben: Cell Samples, Tissue Lysate

Analytische Methode: Semi-Quantitative

Spezifität: This ELISA kit recognizes Human Estrogen receptor phosphorylated at site Serine-118 as well as total Estrogen receptor .

Produktmerkmale:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Anti-Phospho Antibody
• Anti-Pan Antibody
• HRP-Conjugated Secondary Antibody
• Streptavidin-Conjugated HRP
• Assay Diluent
• TMB One-Step Substrate
• Stop Solution
• Lysis Buffer
• Positive Control Sample

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Anti-Phospho Antibody
• Anti-Pan Antibody
• HRP-Conjugated Secondary Antibody
• Streptavidin-Conjugated HRP
• Assay Diluent
• TMB One-Step Substrate
• Stop Solution
• Lysis Buffer
• Positive Control Sample

Benötigtes Material:

• Distilled or deionized water
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare sample dilutions
• Protease and Phosphatase inhibitors
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• Benchtop rocker or shaker
• Microplate reader capable of measuring absorbance at 450 nm

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents and samples as instructed in the manual.
2. Add 100 μL of sample or positive control to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared primary antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
7. Incubate 1 h at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.

Haltbarkeit: 6 months

Details zu Estrogen Receptor alpha

Target: Estrogen Receptor alpha (ESR1) (Estrogen Receptor 1 (ESR1))

Andere Bezeichnung: Estrogen receptor (ESR1 Produkte)

Synonyme: LOC398734 ELISA Kit, AA420328 ELISA Kit, AU041214 ELISA Kit, ER-alpha ELISA Kit, ER[a] ELISA Kit, ERa ELISA Kit, ERalpha ELISA Kit, ESR ELISA Kit, Estr ELISA Kit, Estra ELISA Kit, Nr3a1 ELISA Kit, NR3A1 ELISA Kit, eralpha ELISA Kit, zfER[a] ELISA Kit, ER ELISA Kit, ESRA ELISA Kit, ESTRR ELISA Kit, Era ELISA Kit, akap12 ELISA Kit, cb27 ELISA Kit, esr1 ELISA Kit, fb72g12 ELISA Kit, id:ibd1202 ELISA Kit, sb:cb27 ELISA Kit, si:ch73-192g21.1 ELISA Kit, wu:fb72g12 ELISA Kit, wu:fc21c03 ELISA Kit, Esr ELISA Kit, RNESTROR ELISA Kit, ERALPHA ELISA Kit, er ELISA Kit, esr ELISA Kit, nr3a1 ELISA Kit, XER ELISA Kit, era ELISA Kit, esra ELISA Kit, ERbeta ELISA Kit, xesr-1 ELISA Kit, xlERalpha1 ELISA Kit, xlERalpha2 ELISA Kit, ESR1 ELISA Kit, estrogen receptor 1 L homeolog ELISA Kit, estrogen receptor 1 (alpha) ELISA Kit, estrogen receptor 1 ELISA Kit, A kinase (PRKA) anchor protein 12b ELISA Kit, esr1.L ELISA Kit, Esr1 ELISA Kit, ESR1 ELISA Kit, esr1 ELISA Kit, akap12b ELISA Kit

Gen-ID: 2099

UniProt: P03372

Pathways: Nuclear Receptor Transcription Pathway, EGFR Signaling Pathway, Retinoic Acid Receptor Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly