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MMP1 ELISA Kit
MMP1
Reaktivität: Human
Colorimetric
Sandwich ELISA
8-18000 pg/mL
Cell Lysate, Tissue Lysate
Produktdetails MMP1 ELISA Kit
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Target
Alle MMP1 ELISA Kits anzeigen
MMP1
(Matrix Metallopeptidase 1 (Interstitial Collagenase) (MMP1))
Reaktivität
Alle Reaktivitäten für MMP1 ELISA Kits
Human
Nachweismethode
Colorimetric
Methodentyp
Sandwich ELISA
Detektionsbereich
8-18000 pg/mL
Untere Nachweisgrenze
8 pg/mL
Applikation
ELISA
Verwendungszweck
Human MMP-1 ELISA Kit for cell and tissue lysate samples.
Proben
Tissue Lysate, Cell Lysate
Analytische Methode
Quantitative
Spezifität
The antibody pair provided in this kit recognizes human MMP-1.
Sensitivität
8 pg/mL
Produktmerkmale
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Bestandteile
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Benötigtes Material
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis Cell lysate buffer
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Anwendungsinformationen
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Probenmenge
100 μL
Plattentyp
Pre-coated
Protokoll
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Handhabung
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Lagerung
-20 °C
Informationen zur Lagerung
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Haltbarkeit
6 months
Details zu MMP1
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Target
Alle MMP1 ELISA Kits anzeigen
MMP1
(Matrix Metallopeptidase 1 (Interstitial Collagenase) (MMP1))
Andere Bezeichnung
MMP-1 (MMP1 Produkte )
Synonyme
CLG ELISA Kit, CLGN ELISA Kit, Mmp1a ELISA Kit, CG4859 ELISA Kit, Dm1-MMP ELISA Kit, Dmel\\CG4859 ELISA Kit, MMP-1 ELISA Kit, MMP1 ELISA Kit, Mmp 1 ELISA Kit, dMMP1 ELISA Kit, dm1-MMP ELISA Kit, dmmp1 ELISA Kit, l(2)k04809 ELISA Kit, mmp1 ELISA Kit, Mmp1 ELISA Kit, Mcol-A ELISA Kit, Mcola ELISA Kit, col4 ELISA Kit, mmp18 ELISA Kit, Clgn ELISA Kit, matrix metallopeptidase 1 ELISA Kit, Matrix metalloproteinase 1 ELISA Kit, matrix metalloproteinase 1 ELISA Kit, matrix metallopeptidase 1 (interstitial collagenase) ELISA Kit, matrix metallopeptidase 1a (interstitial collagenase) ELISA Kit, matrix metallopeptidase 1 S homeolog ELISA Kit, interstitial collagenase ELISA Kit, matrix metallopeptidase 8 L homeolog ELISA Kit, MMP1 ELISA Kit, Mmp1 ELISA Kit, RB11133 ELISA Kit, Mmp1a ELISA Kit, mmp1.S ELISA Kit, LOC100727966 ELISA Kit, mmp8.L ELISA Kit
Hintergrund
Gene Names: MMP1 CLG Protein names: Interstitial collagenase (EC 3.4.24.7) (Fibroblast collagenase) (Matrix metalloproteinase-1) (MMP-1) [Cleaved into: 22 kDa interstitial collagenase, 27 kDa interstitial collagenase]
Gen-ID
4312
UniProt
P03956
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