EGFR ELISA Kit

Produktnummer ABIN625223

Produktdetails EGFR ELISA Kit

Target: EGFR (Epidermal Growth Factor Receptor (EGFR))

Bindungsspezifität: pTyr1068

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Human Phospho-EGFR (Y1068) ELISA Kit. This assay semi-quantitatively measures phophorylated EGFR (Tyr1045) and Total EGFR in lysate samples.

Proben: Cell Lysate, Tissue Lysate

Analytische Methode: Semi-Quantitative

Spezifität: The antibody pair provided in this kit recognizes human Phospho-EGFR (pTyr1068).

Produktmerkmale:

• Rapidly measure phosphorylated protein in lysates
• Screen numerous different cell lysates without performing a Western Blot analysis
• Minimal hands-on time, convenient, and non-radioactive material

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Anti-Phospho Antibody
• HRP-Conjugated Secondary Antibody
• Assay Diluent
• TMB One-Step Substrate
• Stop Solution
• Lysis Buffer
• Positive Control Sample

Benötigtes Material:

• Distilled or deionized water
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare sample dilutions
• Protease and Phosphatase inhibitors
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• Benchtop rocker or shaker
• Microplate reader capable of measuring absorbance at 450 nm

Anwendungsinformationen

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents and samples as instructed in the manual.
2. Add 100 μL of sample or positive control to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared primary antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
7. Incubate 1 h at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Aufbereitung der Reagenzien:

1. Bring all reagents and samples to room temperature (18 - 25 °C) before use.
   2. Item D, Assay Diluent should be diluted 5-fold with deionized or distilled water before use.
   3. Preparation of Positive Control: Briefly spin the Positive Control vial of Item K. Add 400 µL 1x Assay Diluent (Item E, Assay Diluent should be diluted 5-fold with deionized or distilled water before use) into Item K vial to prepare P-1 (See i. Positive control of part IX.for a typical result). Dissolve the powder thoroughly by a gentle mix (it can be removed by centrifuge if any precipitate in the solution is found). Pipette 300 µL 1x Assay Diluent into each tube. Add 100 µL prepared Positive Control P-1 into a tube with 300 µL 1x Asaay Diluent to produce a dilution series (shown below). Mix each tube thoroughly before the next transfer. 1x Assay Diluent serves as the background. Human Phospho-EGFR (Tyr 1068) ELISA Kit Protocol 6
   4. If the Wash Concentrate (20x) (Item B) contains visible crystals, warm to room temperature and mix gently until dissolved. Dilute 20 mL of Wash Buffer Concentrate into deionized or distilled water to yield 400 mL of 1x Wash Buffer.
   5. Briefly spin the anti-phospho-EGFR (Tyr 1068) (Item C) before use. Pipette up and down to mix gently. The anti- phospho-EGFR (Tyr 1068) should be diluted 1,000-fold with 1x Assay Diuent. For example, add 5 µL anti-phospho-EGFR (Tyr 1068) into a tube with 5.0 mL 1x Assay Diluent to prepare a 1,000-fold diluted antibody.
   6. Briefly spin the HRP-conjugated anti-rabbit IgG (Item D-1) before use. Pipette up and down to mix gently. HRP-conjugated anti-rabbit IgG concentrate should be diluted 500-fold with 1x Assay Diuent. For example: Briefly spin the vial (Item D-1) and pipette up and down to mix gently. Add 10 µL of HRP-conjugated anti- rabbit IgG concentrate into a tube with 5.0 mL 1x Assay Diluent to prepare a 500-fold diluted HRP-conjugated anti- P (1) P (2) P (3) P (4) 0 100 µL Positive Control 400 µL 1x Assay Diluent 100µl 100 µL Human Phospho-EGFR (Tyr 1068) ELISA Kit Protocol 7 rabbit IgG solution.
   7. Cell lysis buffer should be diluted 2-folds with deionized or distilled water before use (recommend to add protease and phosphatase inhibitors). VII.

Aufbereitung der Proben:

Cell lysates - Rinse cells with PBS, making sure to remove any remaining PBS before adding the lysis buffer. Solubilize cells at 2 x 107 cells/mL in 1x Lysis Buffer (we recommend adding protease and phosphatase inhibitors to lysis buffer prior to sample preparation). Pipette up and down to resuspend and incubate the lysates with shaking at 2 - 8° C for 30 minutes. Microcentrifuge at 13,000 rpm for 10 minutes at 2 - 8° C, and transfer the supernates into a clean test tube. Lysates should be used immediately or aliquoted and stored at -70 °C. Avoid repeated freeze-thaw cycles. Thawed lysates should be kept on ice prior to use.
For the initial experiment, we recommend to do a serial dilution testing such as 5-fold and 50-fold dilution for your cell lysates with Assay Diluent (Item E) before use.
Note: The fold dilution of sample used depends on the abundance of phosphorylated proteins and should be determined empiricallys. Human Phospho-EGFR (Tyr 1068) ELISA Kit Protocol 5 More of the sample can be used if signals are too weak. If signals are too strong, the sample can be diluted further.
Cell lysis buffer should be diluted 2-fold with deionized or distilled water before use (recommend to add protease and phosphatase inhibitors).

Testdurchführung:

1. Bring all reagents to room temperature (18 - 25 °C) before use. It is recommended that all samples or Positive Control should be run at least in duplicate.
   2. Add 100 µL of each sample or positive control into appropriate wells. Cover well with plate holder and incubate for 2.5 hours at room temperature or over night at 4 °C with shaking.
   3. Discard the solution and wash 4 times with 1x Wash Solution. Wash by filling each well with Wash Buffer (300 µL) using a multi-channel Pipette or autowasher. Complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining Wash Buffer by aspirating or decanting. Invert the plate and blot it against clean paper towels.
   4. Add 100 µL of prepared 1X primary antibody (Reagent Preparation step 5) to each well. Incubate for 1.5 hour at room temperature with shaking.
   5. Discard the solution. Repeat the wash as in step3. Human Phospho-EGFR (Tyr 1068) ELISA Kit Protocol 8
   6. Add 100 µL of prepared 1X secondary antibody solution (see Reagent Preparation step 6) to each well. Incubate for over night at 4 °C with shaking.
   7. Discard the solution. Repeat the wash as in step3.
   8. Add 100 µL of TMB One-Step Substrate Reagent (Item H) to each well. Incubate for 30 minutes at room temperature in the dark with shaking.
   9. Add 50 µL of Stop Solution (Item I) to each well. Read at 450 nm immediately.

Ergebnisberechnung:

ELISA data analysis: Average the duplicate readings for each sample or positive control then subtract the average blank optical density.
i. Positive Control A431 cells were treated with recombinant human EGF at 37 °C for 10 min. Solubilize cells at 4 x 107 cells/mL in lysis buffer. Serial dilutions of lysates were analyzed in this ELISA. Please see step 3 of Part VI Reagent Preparation for detail. Assay Diluent Positive control O D = 4 5 0 n m 1 P (1) P(2) P(3) P(4) 0 - - 0.5 0 Human Phospho-EGFR (Tyr 1068) ELISA Kit Protocol 10
ii. Recombinant Human EGF Stimulation of A431 Cell Lines A431 cells were treated or untreated with 100 ng/mL recombinant human EGF for 10 min. Cell lysates were analyzed using this phosphoELISA and Western Blot. ELISA Phospho-EGFR (Tyr1068) EGFR O D =4 50 n m 0.0 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 Untreated A431 EGF treated A431 Western-Blot hEGF 0 10 0 10 (Min) Anti-phospho-EGFR Anti-EGFR (Tyr1068) Human Phospho-EGFR (Tyr 1068) ELISA Kit Protocol 11 X

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid repeated freeze- thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.

Haltbarkeit: 6 months

Referenzen für EGFR ELISA Kit (ABIN625223)

Katsila, Juliachs, Gregori, Macarulla, Villarreal, Bardelli, Torrance, Elez, Tabernero, Villanueva: "Circulating pEGFR is a candidate response biomarker of cetuximab therapy in colorectal cancer." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 20, Issue 24, pp. 6346-56, (2014) (PubMed).

Maurya, Rai, Rai, Deshpande, Jain, Mudiam, Prabhakar, Bandyopadhyay: "Cypermethrin induces astrocyte apoptosis by the disruption of the autocrine/paracrine mode of epidermal growth factor receptor signaling." in: Toxicological sciences : an official journal of the Society of Toxicology, Vol. 125, Issue 2, pp. 473-87, (2012) (PubMed).

Details zu EGFR

Target: EGFR (Epidermal Growth Factor Receptor (EGFR))

Andere Bezeichnung: EGFR (EGFR Produkte)

Synonyme: C-erb ELISA Kit, CG10079 ELISA Kit, D-EGFR ELISA Kit, D-Egf ELISA Kit, DEGFR ELISA Kit, DER ELISA Kit, DER flb ELISA Kit, DER/EGFR ELISA Kit, DER/faint little ball ELISA Kit, DER/top ELISA Kit, DER/torpedo ELISA Kit, DER1 ELISA Kit, DEgfr ELISA Kit, Degfr ELISA Kit, Der ELISA Kit, DmHD-33 ELISA Kit, Dmel\\CG10079 ELISA Kit, EFG-R ELISA Kit, EGF-R ELISA Kit, EGFR ELISA Kit, EGFr ELISA Kit, EGfr ELISA Kit, EK2-6 ELISA Kit, Egf ELISA Kit, Egf-r ELISA Kit, EgfR ELISA Kit, El ELISA Kit, Elp ELISA Kit, Elp-1 ELISA Kit, Elp-B1 ELISA Kit, Elp-B1RB1 ELISA Kit, HD-33 ELISA Kit, TOP ELISA Kit, Torpedo/DER ELISA Kit, Torpedo/Egfr ELISA Kit, c-erbB ELISA Kit, d-egf-r ELISA Kit, dEGFR ELISA Kit, dEGFR1 ELISA Kit, dEgfr ELISA Kit, der ELISA Kit, egfr ELISA Kit, flb ELISA Kit, l(2)05351 ELISA Kit, l(2)09261 ELISA Kit, l(2)57DEFa ELISA Kit, l(2)57EFa ELISA Kit, l(2)57Ea ELISA Kit, mor1 ELISA Kit, top ELISA Kit, top/DER ELISA Kit, top/flb ELISA Kit, torpedo/Egfr ELISA Kit, torpedo/egfr ELISA Kit, EGFR12 ELISA Kit, EGFR15 ELISA Kit, egfr1 ELISA Kit, Erbb2 ELISA Kit, ERBB ELISA Kit, ERBB1 ELISA Kit, HER1 ELISA Kit, PIG61 ELISA Kit, mENA ELISA Kit, ErbB-1 ELISA Kit, Errp ELISA Kit, 9030024J15Rik ELISA Kit, AI552599 ELISA Kit, Erbb ELISA Kit, Errb1 ELISA Kit, Wa5 ELISA Kit, wa-2 ELISA Kit, wa2 ELISA Kit, epidermal growth factor receptor ELISA Kit, Epidermal growth factor receptor ELISA Kit, epidermal growth factor receptor a (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian) ELISA Kit, EGFR ELISA Kit, Egfr ELISA Kit, egfra ELISA Kit, egfr1 ELISA Kit, LOC5564544 ELISA Kit

Hintergrund: EGFR-Y1068

Gen-ID: 3236

UniProt: P00533

Pathways: NF-kappaB Signalweg, RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Stem Cell Maintenance, Hepatitis C, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation, S100 Proteine