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IL5RA ELISA Kit
IL5RA
Reaktivität: Human
Colorimetric
Sandwich ELISA
0.15-40 ng/mL
Cell Culture Supernatant, Plasma, Serum
Produktdetails IL5RA ELISA Kit
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Target
Alle IL5RA ELISA Kits anzeigen
IL5RA
(Interleukin 5 Receptor, alpha (IL5RA))
Reaktivität
Alle Reaktivitäten für IL5RA ELISA Kits
Human
Nachweismethode
Colorimetric
Methodentyp
Sandwich ELISA
Detektionsbereich
0.15-40 ng/mL
Untere Nachweisgrenze
0.15 ng/mL
Applikation
ELISA
Verwendungszweck
Human IL-5 R alpha ELISA Kit for cell culture supernatants, plasma, and serum samples.
Proben
Plasma, Cell Culture Supernatant, Serum
Analytische Methode
Quantitative
Spezifität
This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, CNTF, ENA- 78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, FGF-4, FGF-6, FGF- 7, G-CSF, GDNF, GM-CSF, IFN-gamma, IGFBP-2, IGFBP-3, IGFBP-4, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIF, MIG, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, SDF-1 alpha, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Sensitivität
150 pg/mL
Produktmerkmale
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Bestandteile
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Benötigtes Material
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Applikationshinweise
Recommended Dilution for serum and plasma samples2 fold
Probenmenge
100 μL
Plattentyp
Pre-coated
Protokoll
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Handhabung
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Lagerung
-20 °C
Informationen zur Lagerung
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Haltbarkeit
6 months
Details zu IL5RA
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Target
Alle IL5RA ELISA Kits anzeigen
IL5RA
(Interleukin 5 Receptor, alpha (IL5RA))
Andere Bezeichnung
IL-5 R alpha (IL5RA Produkte )
Synonyme
MGC86517 ELISA Kit, IL5RA ELISA Kit, il5r ELISA Kit, cdw125 ELISA Kit, hsil5r3 ELISA Kit, gpIL-5ra ELISA Kit, CD125 ELISA Kit, CDw125 ELISA Kit, HSIL5R3 ELISA Kit, IL5R ELISA Kit, Il5r ELISA Kit, interleukin 5 receptor subunit alpha L homeolog ELISA Kit, interleukin 5 receptor subunit alpha ELISA Kit, interleukin 5 receptor, alpha ELISA Kit, il5ra.L ELISA Kit, IL5RA ELISA Kit, il5ra ELISA Kit, Il5ra ELISA Kit
Hintergrund
Gene Names: IL5RA IL5R Protein names: Interleukin-5 receptor subunit alpha (IL-5 receptor subunit alpha) (IL-5R subunit alpha) (IL-5R-alpha) (IL-5RA) (CDw125) (CD antigen CD125)
Gen-ID
3568
UniProt
Q01344
Pathways
JAK-STAT Signalweg
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