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LYVE1 ELISA Kit

LYVE1 Reaktivität: Human Colorimetric Sandwich ELISA 8-2000 pg/mL Cell Culture Supernatant, Plasma, Serum
Produktnummer ABIN625334
  • Target Alle LYVE1 ELISA Kits anzeigen
    LYVE1 (Lymphatic Vessel Endothelial Hyaluronan Receptor 1 (LYVE1))
    Reaktivität
    • 5
    • 2
    • 1
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    8-2000 pg/mL
    Untere Nachweisgrenze
    8 pg/mL
    Applikation
    ELISA
    Verwendungszweck
    Human LYVE-1 ELISA Kit for cell culture supernatants, plasma, and serum samples.
    Proben
    Plasma, Cell Culture Supernatant, Serum
    Analytische Methode
    Quantitative
    Spezifität
    This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, ENA-78, FGF- 4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
    Sensitivität
    8 pg/mL
    Produktmerkmale
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Bestandteile
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Benötigtes Material
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • Applikationshinweise
    Recommended Dilution for serum and plasma samples100 - 2,000 fold
    Probenmenge
    100 μL
    Plattentyp
    Pre-coated
    Protokoll
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    -20 °C
    Informationen zur Lagerung
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Haltbarkeit
    6 months
  • Target Alle LYVE1 ELISA Kits anzeigen
    LYVE1 (Lymphatic Vessel Endothelial Hyaluronan Receptor 1 (LYVE1))
    Andere Bezeichnung
    LYVE-1 (LYVE1 Produkte)
    Synonyme
    LYVE1 ELISA Kit, XLKD1 ELISA Kit, CRSBP-1 ELISA Kit, HAR ELISA Kit, LYVE-1 ELISA Kit, 1200012G08Rik ELISA Kit, Crsbp-1 ELISA Kit, Lyve-1 ELISA Kit, Xlkd1 ELISA Kit, lymphatic vessel endothelial hyaluronic receptor 1a ELISA Kit, lymphatic vessel endothelial hyaluronan receptor 1 ELISA Kit, lyve1a ELISA Kit, LYVE1 ELISA Kit, Lyve1 ELISA Kit
    Hintergrund
    Gene Names: LYVE1 CRSBP1 HAR XLKD1 UNQ230/PRO263
    Protein names: Lymphatic vessel endothelial hyaluronic acid receptor 1 (LYVE-1) (Cell surface retention sequence-binding protein 1) (CRSBP-1) (Extracellular link domain-containing protein 1) (Hyaluronic acid receptor)
    Gen-ID
    10894
    UniProt
    Q9Y5Y7
    Pathways
    Glycosaminoglycan Metabolic Process
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