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LYVE1 ELISA Kit
LYVE1
Reaktivität: Human
Colorimetric
Sandwich ELISA
8-2000 pg/mL
Cell Culture Supernatant, Plasma, Serum
Produktdetails LYVE1 ELISA Kit
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Target
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LYVE1
(Lymphatic Vessel Endothelial Hyaluronan Receptor 1 (LYVE1))
Reaktivität
Alle Reaktivitäten für LYVE1 ELISA Kits
Human
Nachweismethode
Colorimetric
Methodentyp
Sandwich ELISA
Detektionsbereich
8-2000 pg/mL
Untere Nachweisgrenze
8 pg/mL
Applikation
ELISA
Verwendungszweck
Human LYVE-1 ELISA Kit for cell culture supernatants, plasma, and serum samples.
Proben
Plasma, Cell Culture Supernatant, Serum
Analytische Methode
Quantitative
Spezifität
This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, ENA-78, FGF- 4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Sensitivität
8 pg/mL
Produktmerkmale
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Bestandteile
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Benötigtes Material
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Anwendungsinformationen
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Applikationshinweise
Recommended Dilution for serum and plasma samples100 - 2,000 fold
Probenmenge
100 μL
Plattentyp
Pre-coated
Protokoll
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Handhabung
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Lagerung
-20 °C
Informationen zur Lagerung
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Haltbarkeit
6 months
Details zu LYVE1
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Target
Alle LYVE1 ELISA Kits anzeigen
LYVE1
(Lymphatic Vessel Endothelial Hyaluronan Receptor 1 (LYVE1))
Andere Bezeichnung
LYVE-1 (LYVE1 Produkte )
Synonyme
LYVE1 ELISA Kit, XLKD1 ELISA Kit, CRSBP-1 ELISA Kit, HAR ELISA Kit, LYVE-1 ELISA Kit, 1200012G08Rik ELISA Kit, Crsbp-1 ELISA Kit, Lyve-1 ELISA Kit, Xlkd1 ELISA Kit, lymphatic vessel endothelial hyaluronic receptor 1a ELISA Kit, lymphatic vessel endothelial hyaluronan receptor 1 ELISA Kit, lyve1a ELISA Kit, LYVE1 ELISA Kit, Lyve1 ELISA Kit
Hintergrund
Gene Names: LYVE1 CRSBP1 HAR XLKD1 UNQ230/PRO263 Protein names: Lymphatic vessel endothelial hyaluronic acid receptor 1 (LYVE-1) (Cell surface retention sequence-binding protein 1) (CRSBP-1) (Extracellular link domain-containing protein 1) (Hyaluronic acid receptor)
Gen-ID
10894
UniProt
Q9Y5Y7
Pathways
Glycosaminoglycan Metabolic Process
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