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TNFRSF10A ELISA Kit
TNFRSF10A
Reaktivität: Human
Colorimetric
Sandwich ELISA
40-10.000 pg/mL
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Produktdetails TNFRSF10A ELISA Kit
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Target
Alle TNFRSF10A ELISA Kits anzeigen
TNFRSF10A
(Tumor Necrosis Factor Receptor Superfamily, Member 10a (TNFRSF10A))
Reaktivität
Alle Reaktivitäten für TNFRSF10A ELISA Kits
Human
Nachweismethode
Colorimetric
Methodentyp
Sandwich ELISA
Detektionsbereich
40-10.000 pg/mL
Untere Nachweisgrenze
40 pg/mL
Applikation
ELISA
Verwendungszweck
Custom Human TRAIL Receptor 1 ELISA Kit.
Proben
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Analytische Methode
Quantitative
Spezifität
The antibody pair provided in this kit recognizes human TRAIL R1.
Sensitivität
40 pg/mL
Produktmerkmale
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Bestandteile
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Benötigtes Material
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Anwendungsinformationen
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Probenmenge
100 μL
Plattentyp
Pre-coated
Protokoll
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Handhabung
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Lagerung
-20 °C
Informationen zur Lagerung
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Haltbarkeit
6 months
Details zu TNFRSF10A
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Target
Alle TNFRSF10A ELISA Kits anzeigen
TNFRSF10A
(Tumor Necrosis Factor Receptor Superfamily, Member 10a (TNFRSF10A))
Andere Bezeichnung
TRAIL R1 (TNFRSF10A Produkte )
Synonyme
APO2 ELISA Kit, CD261 ELISA Kit, DR4 ELISA Kit, TRAILR-1 ELISA Kit, TRAILR1 ELISA Kit, TNFRSF10A ELISA Kit, TNF receptor superfamily member 10a ELISA Kit, tumor necrosis factor receptor superfamily member 26 ELISA Kit, tumor necrosis factor receptor superfamily, member 10a ELISA Kit, TNFRSF10A ELISA Kit, LOC510798 ELISA Kit
Hintergrund
Gene Names: TNFSF10 APO2L TRAIL Protein names: Tumor necrosis factor ligand superfamily member 10 (Apo-2 ligand) (Apo-2L) (TNF-related apoptosis-inducing ligand) (Protein TRAIL) (CD antigen CD253)
Gen-ID
8797
UniProt
O00220
Pathways
Apoptose , Positive Regulation of Endopeptidase Activity
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