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TNFRSF19 ELISA Kit
TNFRSF19
Reaktivität: Human
Colorimetric
Sandwich ELISA
50 pg/mL-400 ng/mL
Cell Culture Supernatant, Plasma, Serum
Produktdetails TNFRSF19 ELISA Kit
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Target
Alle TNFRSF19 ELISA Kits anzeigen
TNFRSF19
(Tumor Necrosis Factor Receptor Superfamily, Member 19 (TNFRSF19))
Reaktivität
Alle Reaktivitäten für TNFRSF19 ELISA Kits
Human
Nachweismethode
Colorimetric
Methodentyp
Sandwich ELISA
Detektionsbereich
50 pg/mL-400 ng/mL
Untere Nachweisgrenze
50 pg/mL
Applikation
ELISA
Verwendungszweck
Human TROY (TNFRSF19) ELISA Kit for cell culture supernatants, plasma, and serum samples.
Proben
Plasma, Cell Culture Supernatant, Serum
Analytische Methode
Quantitative
Spezifität
This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, CNTF, ENA- 78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, FGF-4, FGF-6, FGF- 7, G-CSF, GDNF, GM-CSF, IFN-gamma, IGFBP-2, IGFBP-3, IGFBP-4, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIF, MIG, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, SDF-1 alpha, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Sensitivität
50 pg/mL
Produktmerkmale
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Bestandteile
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Benötigtes Material
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Anwendungsinformationen
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Applikationshinweise
Recommended Dilution for serum and plasma samples2 - 10 fold
Probenmenge
100 μL
Plattentyp
Pre-coated
Protokoll
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Handhabung
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Lagerung
-20 °C
Informationen zur Lagerung
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Haltbarkeit
6 months
Details zu TNFRSF19
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Target
Alle TNFRSF19 ELISA Kits anzeigen
TNFRSF19
(Tumor Necrosis Factor Receptor Superfamily, Member 19 (TNFRSF19))
Andere Bezeichnung
TROY (TNFRSF19 Produkte )
Synonyme
TNFRSF19 ELISA Kit, DKFZp468A0320 ELISA Kit, TAJ ELISA Kit, TAJ-alpha ELISA Kit, TRADE ELISA Kit, TROY ELISA Kit, AL023044 ELISA Kit, AW123854 ELISA Kit, TAJ-ALPHA ELISA Kit, Troy ELISA Kit, RGD1564996 ELISA Kit, TNF receptor superfamily member 19 ELISA Kit, tumor necrosis factor receptor superfamily, member 19 ELISA Kit, TNFRSF19 ELISA Kit, Tnfrsf19 ELISA Kit
Hintergrund
Gene Names: TNFRSF19 TAJ TROY UNQ1888/PRO4333 Protein names: Tumor necrosis factor receptor superfamily member 19 (TRADE) (Toxicity and JNK inducer)
Gen-ID
55504
UniProt
Q9NS68
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