TYRO3 ELISA Kit

Produktnummer ABIN625385

Produktdetails TYRO3 ELISA Kit

Target: TYRO3 (TYRO3 Protein Tyrosine Kinase (TYRO3))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.4-100 ng/mL

Untere Nachweisgrenze: 0.4 ng/mL

Applikation: ELISA

Verwendungszweck: Mouse Dtk ELISA Kit for cell culture supernatants, plasma, and serum samples.

Proben: Cell Culture Supernatant, Plasma, Serum

Analytische Methode: Quantitative

Spezifität: Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN- ?, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 ?, IL-1?, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP-5, M-CSF, MIG, MIP-1?, MIP-1?, MIP-2, MIP-3?, MIP-3?, PF-4, P-Selectin, RANTES, SCF, SDF-1?, TARC, TCA-3, TECK, TIMP-1, TNF-?, TNF RI, TNF RII, TPO, VCAM-1, VEGF.

Sensitivität: 0.4 ng/mL

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Applikationshinweise: Recommended Dilution for serum and plasma samples2 fold

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Haltbarkeit: 6 months

Details zu TYRO3

Target: TYRO3 (TYRO3 Protein Tyrosine Kinase (TYRO3))

Andere Bezeichnung: Dtk (TYRO3 Produkte)

Synonyme: TYRO3 ELISA Kit, brt ELISA Kit, byk ELISA Kit, dtk ELISA Kit, rse ELISA Kit, sky ELISA Kit, tif ELISA Kit, Xksy ELISA Kit, MGC145734 ELISA Kit, DKFZp469I0432 ELISA Kit, LOC100231598 ELISA Kit, BYK ELISA Kit, Dtk ELISA Kit, RSE ELISA Kit, Sky ELISA Kit, Tif ELISA Kit, Brt ELISA Kit, REK ELISA Kit, ksy ELISA Kit, xksy ELISA Kit, AI323366 ELISA Kit, Etk-2 ELISA Kit, Rse ELISA Kit, TYRO3 protein tyrosine kinase ELISA Kit, TYRO3 protein tyrosine kinase L homeolog ELISA Kit, TYRO3 protein tyrosine kinase 3 ELISA Kit, TYRO3 ELISA Kit, tyro3 ELISA Kit, Tyro3 ELISA Kit, tyro3.L ELISA Kit

Hintergrund: Gene Names: Tyro3 Dtk Rse Tif
Protein names: Tyrosine-protein kinase receptor TYRO3 (EC 2.7.10.1) (Etk2/tyro3) (TK19-2) (Tyrosine-protein kinase DTK) (Tyrosine-protein kinase RSE) (Tyrosine-protein kinase TIF)

Gen-ID: 22174

UniProt: P55144

Pathways: RTK Signalweg