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CCL17 ELISA Kit
CCL17
Reaktivität: Maus
Colorimetric
Sandwich ELISA
4-3000 pg/mL
Cell Culture Supernatant, Plasma, Serum
Produktdetails CCL17 ELISA Kit
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Target
Alle CCL17 ELISA Kits anzeigen
CCL17
(Chemokine (C-C Motif) Ligand 17 (CCL17))
Reaktivität
Alle Reaktivitäten für CCL17 ELISA Kits
Maus
Nachweismethode
Colorimetric
Methodentyp
Sandwich ELISA
Detektionsbereich
4-3000 pg/mL
Untere Nachweisgrenze
4 pg/mL
Applikation
ELISA
Verwendungszweck
Mouse TARC (CCL17) ELISA Kit for cell culture supernatants, plasma, and serum samples.
Proben
Cell Culture Supernatant, Plasma, Serum
Analytische Methode
Quantitative
Spezifität
This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN-gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP-5, M-CSF, MIG, MIP-1 alpha, MIP-1 beta, MIP-2, MIP-3 alpha, MIP-3 beta, PF-4, P-Selectin, RANTES, SCF, SDF-1 alpha, TCA-3, TECK, TIMP-1, TNF-alpha, TNF RI, TNF RII, TPO, VCAM-1, VEGF.
Sensitivität
4 pg/mL
Produktmerkmale
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Bestandteile
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Benötigtes Material
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Anwendungsinformationen
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Applikationshinweise
Recommended Dilution for serum and plasma samples2 fold
Probenmenge
100 μL
Plattentyp
Pre-coated
Protokoll
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Handhabung
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Lagerung
-20 °C
Informationen zur Lagerung
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Haltbarkeit
6 months
Details zu CCL17
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Target
Alle CCL17 ELISA Kits anzeigen
CCL17
(Chemokine (C-C Motif) Ligand 17 (CCL17))
Andere Bezeichnung
TARC (CCL17 Produkte )
Synonyme
A-152E5.3 ELISA Kit, ABCD-2 ELISA Kit, SCYA17 ELISA Kit, TARC ELISA Kit, CCL17 ELISA Kit, Abcd-2 ELISA Kit, Scya17 ELISA Kit, Scya17l ELISA Kit, Tarc ELISA Kit, abcd2 ELISA Kit, C-C motif chemokine ligand 17 ELISA Kit, chemokine (C-C motif) ligand 17 ELISA Kit, ATP-binding cassette sub-family D member 2 ELISA Kit, CCL17 ELISA Kit, Ccl17 ELISA Kit, LOC100537809 ELISA Kit
Hintergrund
Gene Names: Ccl17 ABCD-2 TARC mCG_12286 Protein names: C-C motif chemokine
Gen-ID
20295
UniProt
Q9WUZ6
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