AGRN ELISA Kit

Produktnummer ABIN625443

Produktdetails AGRN ELISA Kit

Target: AGRN (Agrin (AGRN))

Reaktivität: Ratte

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.06-50 ng/mL

Untere Nachweisgrenze: 0.06 ng/mL

Applikation: ELISA

Verwendungszweck: Rat Agrin ELISA Kit for Cell Culture Supernatants, Heparin and/or EDTA treated plasma, and Serum samples. Citrate is not recommended.

Proben: Cell Culture Supernatant, Plasma, Serum

Analytische Methode: Quantitative

Spezifität: The antibody pair provided in this kit recognizes rat Agrin.

Sensitivität: 0.06 ng/mL

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Applikationshinweise: Recommended Dilution for serum and plasma samples2 fold

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Aufbereitung der Proben:

Collect plasma using EDTA and/or Heparin as the anticoagulant. Citrate is not recommended.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Haltbarkeit: 6 months

Details zu AGRN

Target: AGRN (Agrin (AGRN))

Andere Bezeichnung: Agrin (AGRN Produkte)

Synonyme: AGRIN ELISA Kit, agrn ELISA Kit, wu:fb57e07 ELISA Kit, Agrin ELISA Kit, nmf380 ELISA Kit, AGR ELISA Kit, HSPG ELISA Kit, agrin ELISA Kit, LOC465392 ELISA Kit, agrn ELISA Kit, AGRN ELISA Kit, Agrn ELISA Kit

Hintergrund: Gene Names: Agrn Agrin
Protein names: Agrin [Cleaved into: Agrin N-terminal 110 kDa subunit, Agrin C-terminal 110 kDa subunit, Agrin C-terminal 90 kDa fragment (C90), Agrin C-terminal 22 kDa fragment (C22)]

Gen-ID: 25592

UniProt: P25304

Pathways: Glycosaminoglycan Metabolic Process, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development