HSP27 ELISA Kit

Produktnummer ABIN625460

Produktdetails HSP27 ELISA Kit

Target: HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.15-50 ng/mL

Untere Nachweisgrenze: 0.15 ng/mL

Applikation: ELISA

Verwendungszweck: Human HSP27 ELISA Kit for cell culture supernatants, plasma, and serum samples.

Proben: Plasma, Cell Culture Supernatant, Serum

Analytische Methode: Quantitative

Spezifität: This ELISA antibody pair detect human HSP27. Other species not determined.

Sensitivität: 150 pg/mL

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Applikationshinweise: Recommended Dilution for serum and plasma samples3 - 30 fold

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Haltbarkeit: 6 months

Details zu HSP27

Target: HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))

Andere Bezeichnung: HSP27 (HSPB1 Produkte)

Synonyme: CMT2F ELISA Kit, HMN2B ELISA Kit, HS.76067 ELISA Kit, HSP27 ELISA Kit, HSP28 ELISA Kit, Hsp25 ELISA Kit, SRP27 ELISA Kit, 27kDa ELISA Kit, Hsp27 ELISA Kit, cb153 ELISA Kit, cb660 ELISA Kit, hsp1 ELISA Kit, hsp25 ELISA Kit, hsp27 ELISA Kit, id:ibd2821 ELISA Kit, sb:cb660 ELISA Kit, zgc:103437 ELISA Kit, HSPB1 ELISA Kit, cmt2f ELISA Kit, hs.76067 ELISA Kit, hsp28 ELISA Kit, heat shock protein family B (small) member 1 ELISA Kit, heat shock protein 1 ELISA Kit, heat shock protein, alpha-crystallin-related, 1 ELISA Kit, heat shock protein family B (small) member 1 L homeolog ELISA Kit, HSPB1 ELISA Kit, Hspb1 ELISA Kit, hspb1 ELISA Kit, hspb1.L ELISA Kit

Hintergrund: Gene Names: HSPB1 HSP27 HSP28
Protein names: Heat shock protein beta-1 (HspB1) (28 kDa heat shock protein) (Estrogen-regulated 24 kDa protein) (Heat shock 27 kDa protein) (HSP 27) (Stress-responsive protein 27) (SRP27)

Gen-ID: 3315

UniProt: P04792

Pathways: MAPK Signalweg, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, VEGF Signaling