HTRA2 ELISA Kit

Produktnummer ABIN625463

Produktdetails HTRA2 ELISA Kit

Target: HTRA2 (HtrA Serine Peptidase 2 (HTRA2))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.2-50 ng/mL

Untere Nachweisgrenze: 0.2 ng/mL

Applikation: ELISA

Verwendungszweck: Human HTRA2 ELISA Kit for cell culture supernatants, plasma, and serum samples.

Proben: Plasma, Cell Culture Supernatant, Serum

Analytische Methode: Quantitative

Spezifität: This ELISA antibody pair detect human HtrA2. Other species not determined yet.

Sensitivität: 200 pg/mL

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Applikationshinweise: Recommended Dilution for serum and plasma samples2 fold

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Haltbarkeit: 6 months

Details zu HTRA2

Target: HTRA2 (HtrA Serine Peptidase 2 (HTRA2))

Andere Bezeichnung: HTRA2 (HTRA2 Produkte)

Synonyme: CG8464 ELISA Kit, DmHtrA2 ELISA Kit, Dmel\\CG8464 ELISA Kit, HtrA2/Omi ELISA Kit, Omi ELISA Kit, dOmi ELISA Kit, domi ELISA Kit, htrA2 ELISA Kit, omi ELISA Kit, HTRA2 ELISA Kit, PRSS25 ELISA Kit, OMI ELISA Kit, PARK13 ELISA Kit, AI481710 ELISA Kit, Prss25 ELISA Kit, mnd2 ELISA Kit, HTRA2-related serine protease ELISA Kit, HtrA serine peptidase 2 ELISA Kit, HtrA2 ELISA Kit, HTRA2 ELISA Kit, htra2 ELISA Kit, Htra2 ELISA Kit

Hintergrund: Gene Names: HTRA2 OMI PRSS25
Protein names: Serine protease HTRA2, mitochondrial (EC 3.4.21.108) (High temperature requirement protein A2) (HtrA2) (Omi stress-regulated endoprotease) (Serine protease 25) (Serine proteinase OMI)

Gen-ID: 27429

UniProt: O43464

Pathways: Positive Regulation of Endopeptidase Activity