XIAP ELISA Kit

Produktnummer ABIN625470

Produktdetails XIAP ELISA Kit

Target: XIAP (X-Linked Inhibitor of Apoptosis (XIAP))

Reaktivität: Human, Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.25-600 ng/mL

Untere Nachweisgrenze: 0.25 ng/mL

Applikation: ELISA

Verwendungszweck: Human XIAP ELISA Kit for cell culture supernatants, plasma, and serum samples.

Proben: Plasma, Cell Culture Supernatant, Serum

Analytische Methode: Quantitative

Spezifität: This ELISA antibody pair can recognize human and mouse XIAP.

Sensitivität: 250 pg/mL

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Applikationshinweise: Recommended Dilution for serum and plasma samples2 fold

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Haltbarkeit: 6 months

Details zu XIAP

Target: XIAP (X-Linked Inhibitor of Apoptosis (XIAP))

Andere Bezeichnung: XIAP (XIAP Produkte)

Synonyme: API3 ELISA Kit, BIRC4 ELISA Kit, IAP-3 ELISA Kit, ILP1 ELISA Kit, MIHA ELISA Kit, XLP2 ELISA Kit, hIAP-3 ELISA Kit, hIAP3 ELISA Kit, api3 ELISA Kit, birc4 ELISA Kit, ilp1 ELISA Kit, miha ELISA Kit, xlp2 ELISA Kit, xxiap ELISA Kit, 1110015C02Rik ELISA Kit, Aipa ELISA Kit, Api3 ELISA Kit, Birc4 ELISA Kit, IAP3 ELISA Kit, ILP-1 ELISA Kit, mXIAP ELISA Kit, riap3 ELISA Kit, fb73d01 ELISA Kit, wu:fb73d01 ELISA Kit, zgc:91873 ELISA Kit, BIRC8 ELISA Kit, X-linked inhibitor of apoptosis ELISA Kit, X-linked inhibitor of apoptosis L homeolog ELISA Kit, XIAP ELISA Kit, xiap ELISA Kit, xiap.L ELISA Kit, Xiap ELISA Kit

Hintergrund: Gene Names: XIAP API3 BIRC4 IAP3
Protein names: E3 ubiquitin-protein ligase XIAP (EC 6.3.2.-) (Baculoviral IAP repeat-containing protein 4) (IAP-like protein) (ILP) (hILP) (Inhibitor of apoptosis protein 3) (IAP-3) (hIAP-3) (hIAP3) (X-linked inhibitor of apoptosis protein) (X-linked IAP)

Gen-ID: 331

UniProt: P98170

Pathways: Apoptose, Caspase Kaskade in der Apoptose, Transition Metal Ion Homeostasis