Testosterone ELISA Kit

Produktnummer ABIN629488

Produktdetails Testosterone ELISA Kit

Target: Testosterone

Reaktivität: Hormone

Methodentyp: Competition ELISA

Applikation: ELISA

Produktmerkmale: ELISA kit for detection of Testosterone in the research laboratory
Alternative Names: Free Testosterone ELISA Kit

Anwendungsinformationen

Applikationshinweise: Optimal conditions to be determined by end user

Testdurchführung:

The principle of the following enzyme immunoassay test follows the typical competitive binding scenario. Competition occurs between an unlabeled antigen (present in standards, control and patient samples) and an enzyme-labelled antigen (conjugate) for a limited number of antibody binding sites on the microwell plate. The washing and decanting procedures remove unbound materials. After the washing step, the enzyme substrate is added. The enzymatic reaction is terminated by addition of the stopping solution. The absorbance is measured on a microtiter plate reader. The intensity of the colour formed is inversely proportional to the concentration of free testosterone in the sample. A set of standards is used to plot a standard curve from which the amount of free testosterone in patient samples and controls can be directly read.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Vorsichtsmaßnahmen: All reagents of this test kit derived from human or animal serum or plasma have been tested and confirmed negative for HIV, HbsAg and HCV. However, there is no test method that can offer complete assurance therefore all reagents, should be treated as potential biohazards in use and for disposal.

Lagerung: 4 °C

Referenzen für Testosterone ELISA Kit (ABIN629488)

Tolman, Lephart, Setchell, Eggett, Christensen: "Timing of supplementation of selenium and isoflavones determines prostate cancer risk factor reduction in rats." in: Nutrition & metabolism, Vol. 5, pp. 31, (2009) (PubMed).

Details zu Testosterone

Target: Testosterone

Abstract: Testosterone Produkte

Substanzklasse: Hormone

Hintergrund: Testosterone is a C-19 steroid secreted from the testis and the adrenal cortex in men and from the adrenal cortex andovaries in women. Testosterone is also produced by peripheral tissues from androstenedione, which is of littlephysiological significance in men, however in women about half of the circulating testosterone is derived from thisorigin. Testosterone measurements are used mainly for clinical evaluation of hypogonadism in males andhyperandrogenic states in females.