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PDPK1 ELISA Kit
PDPK1
Reaktivität: Maus, Human, Ratte
Colorimetric
Cell ELISA
Adherent Cell Culture
Produktnummer ABIN6384200
Produktdetails PDPK1 ELISA Kit
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Target
Alle PDPK1 ELISA Kits anzeigen
PDPK1
(3-phosphoinositide Dependent Protein Kinase-1 (PDPK1))
Reaktivität
Maus, Human, Ratte
Nachweismethode
Colorimetric
Methodentyp
Cell ELISA
Applikation
ELISA
Verwendungszweck
Human/Mouse/Rat Phospho-PDK1 (Ser410) Cell-Based ELISAt. This assay semi-quantitatively measures PDK1 phosphorylated at Serine-410 in adherent cell lines.
Proben
Adherent Cell Culture
Analytische Methode
Semi-Quantitative
Spezifität
This ELISA kit recognizes phosphorylated human, mouse or rat PDK1 (S410) in adherent whole cell lines.
Produktmerkmale
Rapidly measure phosphorylated protein in adherent cell lines Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose) No sample lysis is needed Compatible with a standard ELISA plate reader
Bestandteile
Uncoated 96-well Strip Microplate Wash Buffers Fixing Solution Quenching Buffer Blocking Buffer Anti-phospho antibody Anti-pan antibody HRP-Conjugated Secondary Antibody TMB One-Step Substrate Stop Solution
Benötigtes Material
Distilled or deionized water 100 mL and 1 liter graduated cylinders Tubes to prepare sample dilutions Protease and Phosphatase inhibitors Precision pipettes to deliver 2 μL to 1 mL volumes Adjustable 1-25 mL pipettes for reagent preparation Benchtop rocker or shaker Microplate reader capable of measuring absorbance at 450 nm
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Anwendungsinformationen
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Protokoll
Prepare all reagents and samples as instructed in the manual. Add 100 μL of sample or positive control to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared primary antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared 1X HRP-Streptavidin to each well. Incubate 1 h at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Handhabung
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Lagerung
-20 °C
Informationen zur Lagerung
Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
Haltbarkeit
6 months
Details zu PDPK1
(ausblenden)
Target
Alle PDPK1 ELISA Kits anzeigen
PDPK1
(3-phosphoinositide Dependent Protein Kinase-1 (PDPK1))
Andere Bezeichnung
PDK1 (PDPK1 Produkte )
Synonyme
pdpk1 ELISA Kit, MGC82080 ELISA Kit, PDK1 ELISA Kit, PDPK2 ELISA Kit, PRO0461 ELISA Kit, Pdk1 ELISA Kit, zgc:153787 ELISA Kit, 3'-phosphoinositide-dependent protein kinase 1 ELISA Kit, 3-PHOSPHOINOSITIDE-DEPENDENT PROTEIN KINASE 1 ELISA Kit, ATPDK1 ELISA Kit, AtPDK1 ELISA Kit, T32M21.110 ELISA Kit, T32M21_110 ELISA Kit, zgc:77318 ELISA Kit, 3-phosphoinositide dependent protein kinase 1 ELISA Kit, 3-phosphoinositide dependent protein kinase 1 L homeolog ELISA Kit, 3-phosphoinositide dependent protein kinase-1 ELISA Kit, 3-phosphoinositide dependent protein kinase 1a ELISA Kit, 3-phosphoinositide-dependent protein kinase 1 ELISA Kit, 3'-phosphoinositide-dependent protein kinase 1 ELISA Kit, 3-phosphoinositide dependent protein kinase 1b ELISA Kit, PDPK1 ELISA Kit, pdpk1.L ELISA Kit, pdpk1 ELISA Kit, Pdpk1 ELISA Kit, pdpk1a ELISA Kit, LOC100380750 ELISA Kit, pdk-1 ELISA Kit, PDK1 ELISA Kit, pdpk1b ELISA Kit
Gen-ID
5170
Pathways
PI3K-Akt Signalweg , T-Zell Rezeptor Signalweg , Fc-epsilon Rezeptor Signalübertragung , EGFR Signaling Pathway , Neurotrophin Signalübertragung , Regulation of Leukocyte Mediated Immunity , Positive Regulation of Immune Effector Process , Cell-Cell Junction Organization , Regulation of Cell Size , Skeletal Muscle Fiber Development , CXCR4-mediated Signaling Events , Signaling Events mediated by VEGFR1 and VEGFR2 , VEGFR1 Specific Signals
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