Angiostatin IQ-ELISA Kit

Produktnummer ABIN6384250

Produktdetails Angiostatin IQ-ELISA Kit

Target: Angiostatin (ANG)

Reaktivität: Human

Nachweismethode: qPCR

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Human Immunoquantitative (PCR-Based) Angiostatin ELISA Kit for cell culture supernatants, plasma, and serum samples.

Proben: Cell Culture Supernatant, Plasma, Serum

Analytische Methode: Semi-Quantitative

Sensitivität: 2000 pg/mL

Produktmerkmale: IQ-ELISAs employ specific capture antibodies coated on a 96-well PCR plate. Standards and samples are pipetted into the wells, the target protein in the standards and samples binds to the immobilized antibody. The wells are washed and the detection affinity reagent is added to the wells where it binds to any captured antigen. The wells are washed, and primers and PCR master mix are added to each well. The plate is placed into a real time PCR instrument for cycling and measurement of DNA amplification. The cycle number where amplification is detected is proportional to the amount of affinity detection reagent that bound to captured antigen in each well.

Bestandteile:

• Angiostatin Microplate (Item A): 96 well PCR plate coated with anti-Human Angiostatin
• Wash Buffer I Concentrate (20x) (Item B): 25 ml of 20x concentrated solution
• Standards (Item C): 2 vials of recombinant Human Angiostatin
• Assay Diluent A (Item D): 30 ml diluent buffer, 0.09% sodium azide as preservative. For Standard/Sample (serum/plasma) diluent
• Assay Diluent B (Item E): 15 ml of 5x concentrated buffer. For Standard/Sample (cell culture medium/urine) diluent
• Detection Affinity Reagent for Angiostatin (Item F): 2 vials of a 4x concentrated solution of anti-Human Angiostatin affinity reagent
• IQELISA Detection Reagent (Item G): 1.4ml of a 10x concentrated stock
• Primer Solution (Item I): 1.7 ml vial
• PCR Master Mix (Item J): 1.2 ml vial
• PCR Preparation buffer (Item K): 1ml vial of 10x concentrated buffer
• Final Wash Buffer (Item L): 10ml vial of 10x concentrated buffer

Benötigtes Material:

• Real-time PCR instrument, Bio-Rad recommended
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• 100 mL and 1 liter graduated cylinders
• Absorbent paper
• Distilled or deionized water
• Log-log graph paper or computer and software for data analysis
• Tubes to prepare standard or sample dilutions
• Heating block or water bath capable of 80^°C

Anwendungsinformationen

Applikationshinweise: The Immuno-Quantitative ELISA (IQELISA) kits are an innovative assay platform that combines the specificity and ease of use of an ELISA with the sensitivity of real-time PCR. This results in an assay that is simultaneously familiar and cutting edge and enables the use of only 1/10th the sample volume while also providing 10x more sensitivity than a traditional ELISA.

Kommentare:

The Immuno-Quantitative ELISA (IQELISA™) kits are an innovative assay platform that combines the specificity and ease of use of an ELISA with the sensitivity of real-time PCR. Also called immuno-PCR, this detection platform results in an assay that is simultaneously familiar and cutting edge. Compared to traditional ELISA, IQELISA™ enables the use of only 1/10th the sample volume while also providing 10x more sensitivity.

Probenmenge: 25 µL

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed
2. Add 25 μL standard or sample to each well. Incubate for 2.5 hours at room temperature or overnight at 4^°C
3. Add 25 μL detection affinity reagent to each well. Incubate 1 hour at room temperature
4. Add 25μL of IQELISA Detection Reagent to each well. Incubate 1 hour
5. Add 15μL Primer solution 10μL of PCR master mix to each well
6. Run real-time PCR

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: May be stored for up to 6 months at 2° to 8°C from the date of shipment. Standard (recombinant protein) should be stored at -20°C or -80°C (recommended at -80°C) after reconstitution. Opened PCR plate or reagents may be stored for up to 1 month at 2° to 8°C. Note: the kit can be used within one year if the whole kit is stored at -20°C. Avoid repeated freeze-thaw cycles.

Haltbarkeit: 6 months

Antigendetails

Target: Angiostatin (ANG)

Andere Bezeichnung: Angiostatin (ANG Produkte)

Synonyme: ALS9 IQ-ELISA Kit, HEL168 IQ-ELISA Kit, RNASE4 IQ-ELISA Kit, RNASE5 IQ-ELISA Kit, LPA IQ-ELISA Kit, AI649309 IQ-ELISA Kit, Pg IQ-ELISA Kit, Ab1-346 IQ-ELISA Kit, AI385586 IQ-ELISA Kit, Ang1 IQ-ELISA Kit, Rnase5 IQ-ELISA Kit, Rnase5a IQ-ELISA Kit, angiogenin IQ-ELISA Kit, plasminogen IQ-ELISA Kit, angiogenin, ribonuclease, RNase A family, 5 IQ-ELISA Kit, ANG IQ-ELISA Kit, PLG IQ-ELISA Kit, Plg IQ-ELISA Kit, Ang IQ-ELISA Kit

Gen-ID: 5340

UniProt: P00747

Pathways: Lipid Metabolism