Thymidine Phosphorylase IQ-ELISA Kit

Produktnummer ABIN6384482

Produktdetails Thymidine Phosphorylase IQ-ELISA Kit

Target: Thymidine Phosphorylase (TYMP)

Reaktivität: Human

Nachweismethode: qPCR

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Human Immunoquantitative (PCR-Based) PD-ECGF ELISA Kit for cell culture supernatants, plasma, and serum samples.

Proben: Cell Culture Supernatant, Plasma, Serum

Analytische Methode: Semi-Quantitative

Sensitivität: 0.04 ng/mL

Produktmerkmale: IQ-ELISAs employ specific capture antibodies coated on a 96-well PCR plate. Standards and samples are pipetted into the wells, the target protein in the standards and samples binds to the immobilized antibody. The wells are washed and the detection affinity reagent is added to the wells where it binds to any captured antigen. The wells are washed, and primers and PCR master mix are added to each well. The plate is placed into a real time PCR instrument for cycling and measurement of DNA amplification. The cycle number where amplification is detected is proportional to the amount of affinity detection reagent that bound to captured antigen in each well.

Bestandteile:

• PD-ECGF Microplate (Item A): 96 well PCR plate coated with anti-Human PD-ECGF
• Wash Buffer I Concentrate (20x) (Item B): 25 ml of 20x concentrated solution
• Standards (Item C): 2 vials of recombinant Human PD-ECGF
• Assay Diluent A (Item D): 30 ml diluent buffer, 0.09% sodium azide as preservative. For Standard/Sample (serum/plasma) diluent
• Assay Diluent B (Item E): 15 ml of 5x concentrated buffer. For Standard/Sample (cell culture medium/urine) diluent
• Detection Affinity Reagent for PD-ECGF (Item F): 2 vials of a 4x concentrated solution of anti-Human PD-ECGF affinity reagent
• IQELISA Detection Reagent (Item G): 1.4ml of a 10x concentrated stock
• Primer Solution (Item I): 1.7 ml vial
• PCR Master Mix (Item J): 1.2 ml vial
• PCR Preparation buffer (Item K): 1ml vial of 10x concentrated buffer
• Final Wash Buffer (Item L): 10ml vial of 10x concentrated buffer

Benötigtes Material:

• Real-time PCR instrument, Bio-Rad recommended
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• 100 mL and 1 liter graduated cylinders
• Absorbent paper
• Distilled or deionized water
• Log-log graph paper or computer and software for data analysis
• Tubes to prepare standard or sample dilutions
• Heating block or water bath capable of 80^°C

Anwendungsinformationen

Applikationshinweise: The Immuno-Quantitative ELISA (IQELISA) kits are an innovative assay platform that combines the specificity and ease of use of an ELISA with the sensitivity of real-time PCR. This results in an assay that is simultaneously familiar and cutting edge and enables the use of only 1/10th the sample volume while also providing 10x more sensitivity than a traditional ELISA.

Kommentare:

The Immuno-Quantitative ELISA (IQELISA™) kits are an innovative assay platform that combines the specificity and ease of use of an ELISA with the sensitivity of real-time PCR. Also called immuno-PCR, this detection platform results in an assay that is simultaneously familiar and cutting edge. Compared to traditional ELISA, IQELISA™ enables the use of only 1/10th the sample volume while also providing 10x more sensitivity.

Probenmenge: 25 µL

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed
2. Add 25 μL standard or sample to each well. Incubate for 2.5 hours at room temperature or overnight at 4^°C
3. Add 25 μL detection affinity reagent to each well. Incubate 1 hour at room temperature
4. Add 25μL of IQELISA Detection Reagent to each well. Incubate 1 hour
5. Add 15μL Primer solution 10μL of PCR master mix to each well
6. Run real-time PCR

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: May be stored for up to 6 months at 2° to 8°C from the date of shipment. Standard (recombinant protein) should be stored at -20°C or -80°C (recommended at -80°C) after reconstitution. Opened PCR plate or reagents may be stored for up to 1 month at 2° to 8°C. Note: the kit can be used within one year if the whole kit is stored at -20°C. Avoid repeated freeze-thaw cycles.

Haltbarkeit: 6 months

Antigendetails

Target: Thymidine Phosphorylase (TYMP)

Andere Bezeichnung: PD-ECGF (TYMP Produkte)

Synonyme: ECGF IQ-ELISA Kit, ECGF1 IQ-ELISA Kit, MEDPS1 IQ-ELISA Kit, MNGIE IQ-ELISA Kit, MTDPS1 IQ-ELISA Kit, PDECGF IQ-ELISA Kit, TP IQ-ELISA Kit, hPD-ECGF IQ-ELISA Kit, 2900072D10Rik IQ-ELISA Kit, Ecgf1 IQ-ELISA Kit, PD-ECGF IQ-ELISA Kit, Pdgfec IQ-ELISA Kit, typh IQ-ELISA Kit, thymidine phosphorylase IQ-ELISA Kit, thymidine phosphorylase DeoA IQ-ELISA Kit, AMP phosphorylase IQ-ELISA Kit, Thymidine phosphorylase IQ-ELISA Kit, TYMP IQ-ELISA Kit, Tymp IQ-ELISA Kit, deoA IQ-ELISA Kit, Dd586_0614 IQ-ELISA Kit, Kvar_4413 IQ-ELISA Kit, NMAG_RS19330 IQ-ELISA Kit, Snov_1187 IQ-ELISA Kit, Fbal_2856 IQ-ELISA Kit, Ftrac_2071 IQ-ELISA Kit, Intca_1216 IQ-ELISA Kit, ARCVE_RS10415 IQ-ELISA Kit, Sphch_4174 IQ-ELISA Kit, Sinme_3461 IQ-ELISA Kit, METOK_RS06355 IQ-ELISA Kit, Mesop_1831 IQ-ELISA Kit, typh IQ-ELISA Kit

Gen-ID: 1890

UniProt: P19971

Pathways: Signaling Events mediated by VEGFR1 and VEGFR2