HSPD1 IQ-ELISA Kit

Produktnummer ABIN6384569

Produktdetails HSPD1 IQ-ELISA Kit

Target: HSPD1 (Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1))

Reaktivität: Human, Maus, Ratte

Nachweismethode: qPCR

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Human Immunoquantitative (PCR-Based) HSP60 ELISA Kit for cell culture supernatants, plasma, and serum samples.

Proben: Cell Culture Supernatant, Plasma, Serum

Analytische Methode: Semi-Quantitative

Sensitivität: 0.041 ng/mL

Produktmerkmale: IQ-ELISAs employ specific capture antibodies coated on a 96-well PCR plate. Standards and samples are pipetted into the wells, the target protein in the standards and samples binds to the immobilized antibody. The wells are washed and the detection affinity reagent is added to the wells where it binds to any captured antigen. The wells are washed, and primers and PCR master mix are added to each well. The plate is placed into a real time PCR instrument for cycling and measurement of DNA amplification. The cycle number where amplification is detected is proportional to the amount of affinity detection reagent that bound to captured antigen in each well.

Bestandteile:

• HSP60 Microplate (Item A): 96 well PCR plate coated with anti-Human HSP60
• Wash Buffer I Concentrate (20x) (Item B): 25 ml of 20x concentrated solution
• Standards (Item C): 2 vials of recombinant Human HSP60
• Assay Diluent A (Item D): 30 ml diluent buffer, 0.09% sodium azide as preservative. For Standard/Sample (serum/plasma) diluent
• Assay Diluent B (Item E): 15 ml of 5x concentrated buffer. For Standard/Sample (cell culture medium/urine) diluent
• Detection Affinity Reagent for HSP60 (Item F): 2 vials of a 4x concentrated solution of anti-Human HSP60 affinity reagent
• IQELISA Detection Reagent (Item G): 1.4ml of a 10x concentrated stock
• Primer Solution (Item I): 1.7 ml vial
• PCR Master Mix (Item J): 1.2 ml vial
• PCR Preparation buffer (Item K): 1ml vial of 10x concentrated buffer
• Final Wash Buffer (Item L): 10ml vial of 10x concentrated buffer

Benötigtes Material:

• Real-time PCR instrument, Bio-Rad recommended
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• 100 mL and 1 liter graduated cylinders
• Absorbent paper
• Distilled or deionized water
• Log-log graph paper or computer and software for data analysis
• Tubes to prepare standard or sample dilutions
• Heating block or water bath capable of 80^°C

Anwendungsinformationen

Applikationshinweise: The Immuno-Quantitative ELISA (IQELISA) kits are an innovative assay platform that combines the specificity and ease of use of an ELISA with the sensitivity of real-time PCR. This results in an assay that is simultaneously familiar and cutting edge and enables the use of only 1/10th the sample volume while also providing 10x more sensitivity than a traditional ELISA.

Kommentare:

The Immuno-Quantitative ELISA (IQELISA™) kits are an innovative assay platform that combines the specificity and ease of use of an ELISA with the sensitivity of real-time PCR. Also called immuno-PCR, this detection platform results in an assay that is simultaneously familiar and cutting edge. Compared to traditional ELISA, IQELISA™ enables the use of only 1/10th the sample volume while also providing 10x more sensitivity.

Probenmenge: 25 µL

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed
2. Add 25 μL standard or sample to each well. Incubate for 2.5 hours at room temperature or overnight at 4^°C
3. Add 25 μL detection affinity reagent to each well. Incubate 1 hour at room temperature
4. Add 25μL of IQELISA Detection Reagent to each well. Incubate 1 hour
5. Add 15μL Primer solution 10μL of PCR master mix to each well
6. Run real-time PCR

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: May be stored for up to 6 months at 2° to 8°C from the date of shipment. Standard (recombinant protein) should be stored at -20°C or -80°C (recommended at -80°C) after reconstitution. Opened PCR plate or reagents may be stored for up to 1 month at 2° to 8°C. Note: the kit can be used within one year if the whole kit is stored at -20°C. Avoid repeated freeze-thaw cycles.

Haltbarkeit: 6 months

Antigendetails

Target: HSPD1 (Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1))

Andere Bezeichnung: HSP60 (HSPD1 Produkte)

Synonyme: CPN60 IQ-ELISA Kit, GROEL IQ-ELISA Kit, HLD4 IQ-ELISA Kit, HSP-60 IQ-ELISA Kit, HSP60 IQ-ELISA Kit, HSP65 IQ-ELISA Kit, HuCHA60 IQ-ELISA Kit, SPG13 IQ-ELISA Kit, chaperonin IQ-ELISA Kit, cpn60 IQ-ELISA Kit, groel IQ-ELISA Kit, hld4 IQ-ELISA Kit, hsp60 IQ-ELISA Kit, hsp65 IQ-ELISA Kit, spg13 IQ-ELISA Kit, 60kDa IQ-ELISA Kit, Hsp60 IQ-ELISA Kit, 12 IQ-ELISA Kit, BP5 IQ-ELISA Kit, CG12101 IQ-ELISA Kit, Cpn60 IQ-ELISA Kit, Dmel\\CG12101 IQ-ELISA Kit, Dmhsp60 IQ-ELISA Kit, G62 IQ-ELISA Kit, HSP60A IQ-ELISA Kit, Hsp60A IQ-ELISA Kit, IEF16 IQ-ELISA Kit, Mmp-P1 IQ-ELISA Kit, SSP 7506 IQ-ELISA Kit, d-hsp60 IQ-ELISA Kit, hsp60A IQ-ELISA Kit, l(1)10Ac IQ-ELISA Kit, l(1)BP5 IQ-ELISA Kit, l(1)G8 IQ-ELISA Kit, l(1)HM21 IQ-ELISA Kit, l(1)L12 IQ-ELISA Kit, l(1)dp025 IQ-ELISA Kit, cb863 IQ-ELISA Kit, fa04a05 IQ-ELISA Kit, fb22d10 IQ-ELISA Kit, fi27b05 IQ-ELISA Kit, id:ibd2197 IQ-ELISA Kit, sb:cb144 IQ-ELISA Kit, wu:fa04a05 IQ-ELISA Kit, wu:fb22d10 IQ-ELISA Kit, wu:fi04a12 IQ-ELISA Kit, wu:fi27b05 IQ-ELISA Kit, MIF4 IQ-ELISA Kit, MNA2 IQ-ELISA Kit, mopA IQ-ELISA Kit, groL IQ-ELISA Kit, crpA IQ-ELISA Kit, Hspd1-30p IQ-ELISA Kit, heat shock protein family D (Hsp60) member 1 IQ-ELISA Kit, heat shock protein family D (Hsp60) member 1 S homeolog IQ-ELISA Kit, 60 kDa heat shock protein, mitochondrial IQ-ELISA Kit, heat shock protein 1 (chaperonin) IQ-ELISA Kit, Heat shock protein 60A IQ-ELISA Kit, heat shock 60 protein 1 IQ-ELISA Kit, chaperone ATPase HSP60 IQ-ELISA Kit, molecular chaperone GroEL IQ-ELISA Kit, thermosome subunit IQ-ELISA Kit, chaperonin GroEL IQ-ELISA Kit, mitochondrial chaperonin IQ-ELISA Kit, heat shock protein family D member 1 IQ-ELISA Kit, HSPD1 IQ-ELISA Kit, hspd1.S IQ-ELISA Kit, hspd1 IQ-ELISA Kit, LOC100414401 IQ-ELISA Kit, Hspd1 IQ-ELISA Kit, Hsp60A IQ-ELISA Kit, HSP60 IQ-ELISA Kit, groEL IQ-ELISA Kit, MMP_RS07785 IQ-ELISA Kit, groEl IQ-ELISA Kit, LOC100136430 IQ-ELISA Kit

Gen-ID: 3329

UniProt: P10809

Pathways: Activation of Innate immune Response, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity