CDKN1B IQ-ELISA Kit

Produktnummer ABIN6385359

Produktdetails CDKN1B IQ-ELISA Kit

Target: CDKN1B (Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B))

Reaktivität: Ratte

Nachweismethode: qPCR

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Rat Immunoquantitative (PCR-Based) p27/Kip1 ELISA Kit for cell culture supernatants, plasma, and serum samples.

Proben: Cell Culture Supernatant, Plasma, Serum

Analytische Methode: Semi-Quantitative

Produktmerkmale: IQ-ELISAs employ specific capture antibodies coated on a 96-well PCR plate. Standards and samples are pipetted into the wells, the target protein in the standards and samples binds to the immobilized antibody. The wells are washed and the detection affinity reagent is added to the wells where it binds to any captured antigen. The wells are washed, and primers and PCR master mix are added to each well. The plate is placed into a real time PCR instrument for cycling and measurement of DNA amplification. The cycle number where amplification is detected is proportional to the amount of affinity detection reagent that bound to captured antigen in each well.

Bestandteile:

• p27/Kip1 Microplate (Item A): 96 well PCR plate coated with anti-Rat p27/Kip1
• Wash Buffer I Concentrate (20x) (Item B): 25 ml of 20x concentrated solution
• Standards (Item C): 2 vials of recombinant Rat p27/Kip1
• Assay Diluent A (Item D): 30 ml diluent buffer, 0.09% sodium azide as preservative. For Standard/Sample (serum/plasma) diluent
• Assay Diluent B (Item E): 15 ml of 5x concentrated buffer. For Standard/Sample (cell culture medium/urine) diluent
• Detection Affinity Reagent for p27/Kip1 (Item F): 2 vials of a 4x concentrated solution of anti-Rat p27/Kip1 affinity reagent
• IQELISA Detection Reagent (Item G): 1.4ml of a 10x concentrated stock
• Primer Solution (Item I): 1.7 ml vial
• PCR Master Mix (Item J): 1.2 ml vial
• PCR Preparation buffer (Item K): 1ml vial of 10x concentrated buffer
• Final Wash Buffer (Item L): 10ml vial of 10x concentrated buffer

Benötigtes Material:

• Real-time PCR instrument, Bio-Rad recommended
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• 100 mL and 1 liter graduated cylinders
• Absorbent paper
• Distilled or deionized water
• Log-log graph paper or computer and software for data analysis
• Tubes to prepare standard or sample dilutions
• Heating block or water bath capable of 80^°C

Anwendungsinformationen

Applikationshinweise: The Immuno-Quantitative ELISA (IQELISA) kits are an innovative assay platform that combines the specificity and ease of use of an ELISA with the sensitivity of real-time PCR. This results in an assay that is simultaneously familiar and cutting edge and enables the use of only 1/10th the sample volume while also providing 10x more sensitivity than a traditional ELISA.

Kommentare:

The Immuno-Quantitative ELISA (IQELISA™) kits are an innovative assay platform that combines the specificity and ease of use of an ELISA with the sensitivity of real-time PCR. Also called immuno-PCR, this detection platform results in an assay that is simultaneously familiar and cutting edge. Compared to traditional ELISA, IQELISA™ enables the use of only 1/10th the sample volume while also providing 10x more sensitivity.

Probenmenge: 25 µL

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed
2. Add 25 μL standard or sample to each well. Incubate for 2.5 hours at room temperature or overnight at 4^°C
3. Add 25 μL detection affinity reagent to each well. Incubate 1 hour at room temperature
4. Add 25μL of IQELISA Detection Reagent to each well. Incubate 1 hour
5. Add 15μL Primer solution 10μL of PCR master mix to each well
6. Run real-time PCR

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: May be stored for up to 6 months at 2° to 8°C from the date of shipment. Standard (recombinant protein) should be stored at -20°C or -80°C (recommended at -80°C) after reconstitution. Opened PCR plate or reagents may be stored for up to 1 month at 2° to 8°C. Note: the kit can be used within one year if the whole kit is stored at -20°C. Avoid repeated freeze-thaw cycles.

Haltbarkeit: 6 months

Antigendetails

Target: CDKN1B (Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B))

Andere Bezeichnung: p27/Kip1 (CDKN1B Produkte)

Synonyme: CDKN4 IQ-ELISA Kit, KIP1 IQ-ELISA Kit, MEN1B IQ-ELISA Kit, MEN4 IQ-ELISA Kit, P27KIP1 IQ-ELISA Kit, cdkn1b IQ-ELISA Kit, sb:cb611 IQ-ELISA Kit, wu:fb63g10 IQ-ELISA Kit, wu:fb64g10 IQ-ELISA Kit, wu:fe18e03 IQ-ELISA Kit, CDKN1B IQ-ELISA Kit, Kip1 IQ-ELISA Kit, p27 IQ-ELISA Kit, FAM14D IQ-ELISA Kit, ISG12 IQ-ELISA Kit, ISG12A IQ-ELISA Kit, P27 IQ-ELISA Kit, Cdki1b IQ-ELISA Kit, AA408329 IQ-ELISA Kit, AI843786 IQ-ELISA Kit, p27Kip1 IQ-ELISA Kit, cdkn1bl IQ-ELISA Kit, cyclin dependent kinase inhibitor 1B IQ-ELISA Kit, cyclin-dependent kinase inhibitor 1Bb IQ-ELISA Kit, interferon alpha inducible protein 27 IQ-ELISA Kit, cyclin-dependent kinase inhibitor 1B IQ-ELISA Kit, cyclin-dependent kinase inhibitor 1Ba IQ-ELISA Kit, CDKN1B IQ-ELISA Kit, cdkn1bb IQ-ELISA Kit, IFI27 IQ-ELISA Kit, Cdkn1b IQ-ELISA Kit, cdkn1ba IQ-ELISA Kit

Gen-ID: 83571

UniProt: O08769

Pathways: Zellzyklus, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Sensory Perception of Sound, Mitotic G1-G1/S Phases, DNA Replication, Positive Regulation of Endopeptidase Activity, Synthesis of DNA, Autophagie