POR IQ-ELISA Kit

Produktnummer ABIN6385530

Produktdetails POR IQ-ELISA Kit

Target: POR (P450 (Cytochrome) Oxidoreductase (POR))

Reaktivität: Human

Nachweismethode: qPCR

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Human Immunoquantitative (PCR-Based) POR ELISA Kit for cell culture supernatants, plasma, and serum samples.

Proben: Cell Culture Supernatant, Plasma, Serum

Analytische Methode: Semi-Quantitative

Produktmerkmale: IQ-ELISAs employ specific capture antibodies coated on a 96-well PCR plate. Standards and samples are pipetted into the wells, the target protein in the standards and samples binds to the immobilized antibody. The wells are washed and the detection affinity reagent is added to the wells where it binds to any captured antigen. The wells are washed, and primers and PCR master mix are added to each well. The plate is placed into a real time PCR instrument for cycling and measurement of DNA amplification. The cycle number where amplification is detected is proportional to the amount of affinity detection reagent that bound to captured antigen in each well.

Bestandteile:

• POR Microplate (Item A): 96 well PCR plate coated with anti-Human POR
• Wash Buffer I Concentrate (20x) (Item B): 25 ml of 20x concentrated solution
• Standards (Item C): 2 vials of recombinant Human POR
• Assay Diluent A (Item D): 30 ml diluent buffer, 0.09% sodium azide as preservative. For Standard/Sample (serum/plasma) diluent
• Assay Diluent B (Item E): 15 ml of 5x concentrated buffer. For Standard/Sample (cell culture medium/urine) diluent
• Detection Affinity Reagent for POR (Item F): 2 vials of a 4x concentrated solution of anti-Human POR affinity reagent
• IQELISA Detection Reagent (Item G): 1.4ml of a 10x concentrated stock
• Primer Solution (Item I): 1.7 ml vial
• PCR Master Mix (Item J): 1.2 ml vial
• PCR Preparation buffer (Item K): 1ml vial of 10x concentrated buffer
• Final Wash Buffer (Item L): 10ml vial of 10x concentrated buffer

Benötigtes Material:

• Real-time PCR instrument, Bio-Rad recommended
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• 100 mL and 1 liter graduated cylinders
• Absorbent paper
• Distilled or deionized water
• Log-log graph paper or computer and software for data analysis
• Tubes to prepare standard or sample dilutions
• Heating block or water bath capable of 80^°C

Anwendungsinformationen

Applikationshinweise: The Immuno-Quantitative ELISA (IQELISA) kits are an innovative assay platform that combines the specificity and ease of use of an ELISA with the sensitivity of real-time PCR. This results in an assay that is simultaneously familiar and cutting edge and enables the use of only 1/10th the sample volume while also providing 10x more sensitivity than a traditional ELISA.

Kommentare:

The Immuno-Quantitative ELISA (IQELISA™) kits are an innovative assay platform that combines the specificity and ease of use of an ELISA with the sensitivity of real-time PCR. Also called immuno-PCR, this detection platform results in an assay that is simultaneously familiar and cutting edge. Compared to traditional ELISA, IQELISA™ enables the use of only 1/10th the sample volume while also providing 10x more sensitivity.

Probenmenge: 25 µL

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed
2. Add 25 μL standard or sample to each well. Incubate for 2.5 hours at room temperature or overnight at 4^°C
3. Add 25 μL detection affinity reagent to each well. Incubate 1 hour at room temperature
4. Add 25μL of IQELISA Detection Reagent to each well. Incubate 1 hour
5. Add 15μL Primer solution 10μL of PCR master mix to each well
6. Run real-time PCR

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: May be stored for up to 6 months at 2° to 8°C from the date of shipment. Standard (recombinant protein) should be stored at -20°C or -80°C (recommended at -80°C) after reconstitution. Opened PCR plate or reagents may be stored for up to 1 month at 2° to 8°C. Note: the kit can be used within one year if the whole kit is stored at -20°C. Avoid repeated freeze-thaw cycles.

Haltbarkeit: 6 months

Antigendetails

Target: POR (P450 (Cytochrome) Oxidoreductase (POR))

Andere Bezeichnung: POR (POR Produkte)

Synonyme: CPR IQ-ELISA Kit, CYPOR IQ-ELISA Kit, P450R IQ-ELISA Kit, POR IQ-ELISA Kit, CCR IQ-ELISA Kit, CG11567 IQ-ELISA Kit, DMR IQ-ELISA Kit, DmCPR IQ-ELISA Kit, Dmel\\CG11567 IQ-ELISA Kit, NCPR IQ-ELISA Kit, P450 IQ-ELISA Kit, cpr IQ-ELISA Kit, zgc:110032 IQ-ELISA Kit, npr IQ-ELISA Kit, xpor IQ-ELISA Kit, 4933424M13Rik IQ-ELISA Kit, PORCINO IQ-ELISA Kit, T5J17.90 IQ-ELISA Kit, T5J17_90 IQ-ELISA Kit, TFC C IQ-ELISA Kit, TUBULIN-FOLDING COFACTOR C IQ-ELISA Kit, cytochrome p450 oxidoreductase IQ-ELISA Kit, P450 (cytochrome) oxidoreductase IQ-ELISA Kit, ADP-ribosylation factor interacting protein 2 IQ-ELISA Kit, Cytochrome P450 reductase IQ-ELISA Kit, P450 (cytochrome) oxidoreductase a IQ-ELISA Kit, P450 (cytochrome) oxidoreductase L homeolog IQ-ELISA Kit, NADPH--cytochrome P450 reductase IQ-ELISA Kit, C-CAP/cofactor C-like domain-containing protein IQ-ELISA Kit, POR IQ-ELISA Kit, Arfip2 IQ-ELISA Kit, Por IQ-ELISA Kit, Cpr IQ-ELISA Kit, pora IQ-ELISA Kit, por.L IQ-ELISA Kit, LOC100406157 IQ-ELISA Kit, por IQ-ELISA Kit, LOC100619047 IQ-ELISA Kit

Gen-ID: 5447

UniProt: P16435

Pathways: Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, SARS-CoV-2 Protein Interaktom