TNF alpha ELISA Kit

Produktnummer ABIN6574142

Produktdetails TNF alpha ELISA Kit

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Reaktivität: Kaninchen

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 3.12 pg/mL - 200 pg/mL

Untere Nachweisgrenze: 3.12 pg/mL

Applikation: ELISA

Verwendungszweck: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of TNFa in rabbit serum, plasma, tissue homogenates, cell lysates, cell culture supernates.

We offer validation data (WB) for the kit components. So you can be sure to order a reliable ELISA kit product composed of high quality reagents.

Proben: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Tumor Necrosis Factor Alpha (TNFa)

Kreuzreaktivität (Details): No significant cross-reactivity or interference between Tumor Necrosis Factor Alpha (TNFa) and analogues was observed.

Sensitivität: 1.13 pg/mL

Bestandteile:

• Pre-coated, ready to use 96-well strip plate, flat buttom
• Plate sealer for 96 wells
• Reference Standard
• Standard Diluent
• Detection Reagent A
• Detection Reagent B
• Assay Diluent A
• Assay Diluent B
• Reagent Diluent (if Detection Reagent is lyophilized)
• TMB Substrate
• Stop Solution
• Wash Buffer (30 x concentrate)
• Instruction manual

Anwendungsinformationen

Kommentare:

Information on standard material:
The standard might be recombinant protein or natural protein, that will depend on the specific kit. Moreover, the expression system is E.coli or yeast or mammal cell. There is 0.05% proclin 300 in the standard as preservative.

Information on reagents:
The stop solution used in the kit is sulfuric acid with concentration of 1 mol/L. And the wash solution is TBS. The standard diluent contains 0.02 % sodium azide, assay diluent A and assay diluent B contain 0.01% sodium azide. Some kits can contain is BSA in them.

Information on antibodies:
The provided antibodies and their host vary in different kits.

Probenmenge: 100 μL

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards,
2. Add 100μL standard or sample to each well. Incubate 1 hours at 37 °C,
3. Aspirate and add 100μL prepared Detection Reagent A. Incubate 1 hour at 37 °C,
4. Aspirate and wash 3 times,
5. Add 100μL prepared Detection Reagent B. Incubate 30 minutes at 37 °C,
6. Aspirate and wash 5 times,
7. Add 90μL Substrate Solution. Incubate 10-20 minutes at 37 °C,
8. Add 50μL Stop Solution. Read at 450nm immediately.

Aufbereitung der Reagenzien:

1. Bring all kit components and samples to room temperature (18-25 °C) before use. If the kit will not be used up in one time, please only take out strips and reagents for present experiment, and leave the remaining strips and reagents in required condition.
2. Standard - Reconstitute the Standard with 1.0mL of Standard Diluent, kept for 10 minutes at room temperature, shake gently (not to foam). The concentration of the standard in the stock solution is 800 pg/mL. Please firstly dilute the stock solution to 200pg/mL and the diluted standard serves as the highest standard (200pg/mL). Prepare 7 tubes containing 0.5mL Standard Diluent and produce a double dilution series. Mix each tube thoroughly before the next transfer. Set up 7 points of diluted standard such as 200pg/mL, 100pg/mL, 50pg/mL, 25pg/mL, 12.5pg/mL, 6.25pg/mL, 3.12pg/mL, and the last tube with Standard Diluent is the blank as 0pg/mL.
3. Detection Reagent A and Detection Reagent B - If lyophilized reconstitute the Detection Reagent A with 150μL of Reagent Diluent, keep for 10 minutes at room temperature, shake gently (not to foam). Briefly spin or centrifuge the stock Detection A and Detection B before use. Dilute them to the working concentration 100-fold with Assay Diluent A and B, respectively.
4. Wash Solution - Dilute 20 mL of Wash Solution concentrate (30x) with 580 mL of deionized or distilled water to prepare 600 mL of Wash Solution (1x).
5. TMB substrate - Aspirate the needed dosage of the solution with sterilized tips and do not dump the residual solution into the vial again.

Note:

1. Making serial dilution in the wells directly is not permitted.
2. Prepare standards within 15 minutes before assay. Please do not dissolve the reagents at 37 °C directly.
3. Please carefully reconstitute Standards or working Detection Reagent A and B according to the instruction, and avoid foaming and mix gently until the crystals are completely dissolved. To minimize imprecision caused by pipetting, use small volumes and ensure that pipettors are calibrated. It is recommended to suck more than 10μL for one pipetting.
4. The reconstituted Standards, Detection Reagent A and Detection Reagent B can be used only once.
5. If crystals have formed in the Wash Solution concentrate (30x), warm to room temperature and mix gently until the crystals are completely dissolved.
6. Contaminated water or container for reagent preparation will influence the detection result.

Aufbereitung der Proben:

• It is recommended to use fresh samples without long storage, otherwise protein degradation and denaturation may occur in these samples, leading to false results. Samples should therefore be stored for a short period at 2 - 8 °C or aliquoted at -20 °C (≤1 month) or -80 °C (≤ 3 months). Repeated freeze-thaw cycles should be avoided. Prior to assay, the frozen samples should be slowly thawed and centrifuged to remove precipitates.
• If the sample type is not specified in the instructions, a preliminary test is necessary to determine compatibility with the kit.
• If a lysis buffer is used to prepare tissue homogenates or cell culture supernatant, there is a possibility of causing a deviation due to the introduced chemical substance. The recommended dilution factor is for reference only.
• Please estimate the concentration of the samples before performing the test. If the values are not in the range of the standard curve, the optimal sample dilution for the particular experiment has to be determined.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level of target were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level of target were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV < 10%
Inter-Assay: CV < 12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Vorsichtsmaßnahmen: The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung:

1. For unopened kit: All reagents should be stored according to the labels on the vials. The Standard, Detection Reagent A, Detection Reagent B, and 96-well Strip Plate should be stored at -20 °C upon receipt, while the other reagents should be stored at 4 °C.
2. For opened kits: the remaining reagents must be stored according to the above storage conditions. In addition, please return the unused wells to the foil pouch containing the desiccant and seal the foil pouch with the zipper.

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Haltbarkeit: 6 months

Referenzen für TNF alpha ELISA Kit (ABIN6574142)

Mousa, Allemailem, Alhumaydhi, Alrumaihi, Almatroudi, Aljasir, Alwashmi, Al Rugaie, Soliman, Aljohani, Al Abdulmonem, Ahmed, Khan, Khan, AlSuhaymi, Alsugoor, Al-Megrin, Elsayed: "Cytoprotective Antioxidant, Anti-Inflammatory, and Antifibrotic Impact of Celery Seed Oil and Manuka Honey Against Cyclophosphamide-Induced Cystitis in Rabbits." in: , Vol. 2022, pp. 2863023, (2022) (PubMed).

Mousa, Allemailem, Alhumaydhi, Alrumaihi, Almatroudi, Alwashmi, Aljasir, Al Rugaie, Aljohani, Soliman, Rezk, Hegazy, Seleem: "Could Mesna and Celery Seed Cotherapy Modulate Oxidative Stress and Inflammation of the Urinary Bladder Induced by Ifosfamide in Rabbits?" in: Journal of inflammation research, Vol. 14, pp. 5837-5847, (2021) (PubMed).

Desando, Bartolotti, Cavallo, Schiavinato, Secchieri, Kon, Filardo, Paro, Grigolo: "Short-Term Homing of Hyaluronan-Primed Cells: Therapeutic Implications for Osteoarthritis Treatment." in: Tissue engineering. Part C, Methods, Vol. 24, Issue 2, pp. 121-133, (2018) (PubMed).

Fu, Qiu, Tang, Wang, Cheng, Yin: "Effects of GGCX overexpression on anterior cruciate ligament transection-induced osteoarthritis in rabbits." in: Molecular medicine reports, Vol. 17, Issue 3, pp. 3821-3828, (2018) (PubMed).

Ma, Zhu, Chen, Zhang, Dong, Wu, Lu, Wu: "Sulforaphane, a Natural Isothiocyanate Compound, Improves Cardiac Function and Remodeling by Inhibiting Oxidative Stress and Inflammation in a Rabbit Model of Chronic Heart Failure." in: Medical science monitor : international medical journal of experimental and clinical research, Vol. 24, pp. 1473-1483, (2018) (PubMed).

Duan, Chen, Han, Ren, Wang, Zhao, Li: "The influence of liposomal quercetin on liver damage induced by microwave ablation." in: Scientific reports, Vol. 7, Issue 1, pp. 12677, (2017) (PubMed).

Liu, Pei, Wang, Li, Zhao, Song, Chen, Liu: "The immune impact of mimic endoscopic retrograde appendicitis therapy and appendectomy on rabbits of acute appendicitis." in: Oncotarget, Vol. 8, Issue 39, pp. 66528-66539, (2017) (PubMed).

Mokra, Kosutova, Balentova, Adamkov, Mikolka, Mokry, Antosova, Calkovska: "Effects of budesonide on the lung functions, inflammation and apoptosis in a saline-lavage model of acute lung injury." in: Journal of physiology and pharmacology : an official journal of the Polish Physiological Society, Vol. 67, Issue 6, pp. 919-932, (2017) (PubMed).

Pauchard, Blot, Bruyere, Barbar, Croisier, Piroth, Charles: "Linezolid and atorvastatin impact on pneumonia caused by Staphyloccocus aureus in rabbits with or without mechanical ventilation." in: PLoS ONE, Vol. 12, Issue 11, pp. e0187187, (2017) (PubMed).

Erdi, Keskin, Esen, Kaya, Feyzioglu, Kilinc, Karatas, Cuce, Kalkan: "Telmisartan ameliorates oxidative stress and subarachnoid haemorrhage-induced cerebral vasospasm." in: Neurological research, Vol. 38, Issue 3, pp. 224-31, (2016) (PubMed).

Barbar, Pauchard, Bruyère, Bruillard, Hayez, Croisier, Pugin, Charles: "Mechanical Ventilation Alters the Development of Staphylococcus aureus Pneumonia in Rabbit." in: PLoS ONE, Vol. 11, Issue 7, pp. e0158799, (2016) (PubMed).

Liu, Peng, Fang, Yao, Redmond, Ni: "No midterm advantages in the middle term using small intestinal submucosa and human amniotic membrane in Achilles tendon transverse tenotomy." in: Journal of orthopaedic surgery and research, Vol. 11, Issue 1, pp. 125, (2016) (PubMed).

Mikolka, Kopincová, Košútová, Čierny, Čalkovská, Mokrá: "Lung inflammatory and oxidative alterations after exogenous surfactant therapy fortified with budesonide in rabbit model of meconium aspiration syndrome." in: Physiological research, Vol. 65, Issue Supplementum 5, pp. S653-S662, (2016) (PubMed).

Du, Wu, Qing, Wang, Liang, Yu, Tang: "Systemic and flap inflammatory response associates with thrombosis in flap venous crisis." in: Inflammation, Vol. 38, Issue 1, pp. 298-304, (2015) (PubMed).

Yu, Xie, Xin, Wang: "Panax notoginseng saponins promote wound repair of anterior cruciate ligament through phosphorylation of PI3K, AKT and ERK." in: International journal of clinical and experimental pathology, Vol. 8, Issue 1, pp. 441-9, (2015) (PubMed).

Kaya, Erdi, Kılınc, Keskin, Feyzıoglu, Esen, Karatas, Uyar, Kalkan: "Alterations of the thioredoxin system during subarachnoid hemorrhage-induced cerebral vasospasm." in: Acta neurochirurgica, Vol. 157, Issue 5, pp. 793-9; discussion 799-800, (2015) (PubMed).

García-González, Gutiérrez-Quintanar, Mas-Oliva: "The C-terminal Domain Supports a Novel Function for CETPI as a New Plasma Lipopolysaccharide-Binding Protein." in: Scientific reports, Vol. 5, pp. 16091, (2015) (PubMed).

Kamiyama, Jesmin, Sakuramoto, Shimojyo, Islam, Hagiya, Sugano, Unoki, Oki, Kawano, Mizutani: "Hyperinflation deteriorates arterial oxygenation and lung injury in a rabbit model of ARDS with repeated open endotracheal suctioning." in: BMC anesthesiology, Vol. 15, pp. 73, (2015) (PubMed).

Lin, Chen, Lei, You, Huang, Luo, Li, Zhao, Yan: "Effects of Intravenous Injection of Porphyromonas gingivalis on Rabbit Inflammatory Immune Response and Atherosclerosis." in: Mediators of inflammation, Vol. 2015, pp. 364391, (2015) (PubMed).

Joos, Leucht, Riegger, Hogrefe, Fiedler, Dürselen, Reichel, Ignatius, Brenner: "Differential Interactive Effects of Cartilage Traumatization and Blood Exposure In Vitro and In Vivo." in: The American journal of sports medicine, Vol. 43, Issue 11, pp. 2822-32, (2015) (PubMed).

Details zu TNF alpha

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Andere Bezeichnung: Tumor Necrosis Factor Alpha (TNFa) (TNF alpha Produkte)

Synonyme: DIF ELISA Kit, TNF-alpha ELISA Kit, TNFA ELISA Kit, TNFSF2 ELISA Kit, RATTNF ELISA Kit, Tnfa ELISA Kit, tnf ELISA Kit, TNF-a ELISA Kit, TNFalpha ELISA Kit, Tnfsf1a ELISA Kit, TNFa ELISA Kit, cTNF ELISA Kit, Tnf-alpha ELISA Kit, tnfa-like ELISA Kit, TNF-ALPHA ELISA Kit, dif ELISA Kit, tnfa ELISA Kit, xtnf ELISA Kit, tnfsf2 ELISA Kit, tnf-alpha ELISA Kit, Cachectin ELISA Kit, tumor necrosis factor ELISA Kit, tumor necrosis factor b (TNF superfamily, member 2) ELISA Kit, tumor necrosis factor alpha ELISA Kit, tumor necrosis factor a (TNF superfamily, member 2) ELISA Kit, TNF ELISA Kit, Tnf ELISA Kit, tnf ELISA Kit, tnfb ELISA Kit, tnf-alpha ELISA Kit, LOC103694380 ELISA Kit, tnfa ELISA Kit

UniProt: P04924

Pathways: NF-kappaB Signalweg, Apoptose, Caspase Kaskade in der Apoptose, TLR Signalweg, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome