IL-1 beta ELISA Kit

Produktnummer ABIN6574166

Produktdetails IL-1 beta ELISA Kit

Highlights:

• IL-1 beta-ELISA-Kit für die Maus zum quantitativen Nachweis von IL-1 beta.
• Zuverlässiges Produkt mit validierten Komponenten.
• ELISA-Kit wird in mehr als 40 PubMed-Referenzen zitiert.

Target: IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 15.6 pg/mL - 1000 pg/mL

Untere Nachweisgrenze: 15.6 pg/mL

Applikation: ELISA

Verwendungszweck: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of IL1b in mouse serum, plasma, tissue homogenates, cell lysates, cell culture supernates.

We offer validation data (WB) for the kit components. So you can be sure to order a reliable ELISA kit product composed of high quality reagents.

Proben: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Interleukin 1 Beta (IL1b)

Kreuzreaktivität (Details): No significant cross-reactivity or interference between Interleukin 1 Beta (IL1b) and analogues was observed.

Sensitivität: 5.7 pg/mL

Bestandteile:

• Pre-coated, ready to use 96-well strip plate, flat buttom
• Plate sealer for 96 wells
• Reference Standard
• Standard Diluent
• Detection Reagent A
• Detection Reagent B
• Assay Diluent A
• Assay Diluent B
• Reagent Diluent (if Detection Reagent is lyophilized)
• TMB Substrate
• Stop Solution
• Wash Buffer (30 x concentrate)
• Instruction manual

Produktspezifische Information:

Wofür kann der IL-1 beta Antikörper ABIN6574166 verwendet werden? Der IL-1 beta-ELISA-Kit kann für die Analyse der IL1 beta-Proteinexpression in Zellkulturüberständen, Zelllysaten, Plasma, Serum und Gewebehomogenat verwendet werden. Das erforderliche Probenvolumen beträgt 100 µl. Der ELISA-Kit ist für die Maus validiert und hat einen Nachweisbereich von 15,6 pg/mL - 1000 pg/mL für Interleukin beta 1 mit einer Empfindlichkeit von 5,7 pg/mL. Er zeigt keine signifikante Interferenz oder Kreuzreaktivität zwischen IL-1 beta und Analoga. Verwenden Sie den Maus-IL-1-beta-ELISA-Kit für den quantitativen Nachweis von IL-1-beta mittels Sandwich-ELISA innerhalb von 3 Stunden. Er hat eine hohe Sensitivität und ausgezeichnete Spezifität.

Welche Validierungsdaten sind für diesen IL-1 beta-Antikörper verfügbar? Das Produkt wird in 41 PubMed-Publikationen zitiert. Validierungsbilder für die ELISA-Kit-Komponenten Detektionsantikörper, Capture-Antikörper und Proteinstandard finden Sie oben. Nur für die Forschung im akademischen oder industriellen Bereich geeignet.

Was ist die Funktion von IL-1 beta / Interleukin 1 beta? IL-1 beta (Interleukin 1 beta) gehört zur Familie der Interleukine und ist ein starkes proinflammatorisches Zytokin. Ursprünglich als wichtigstes endogenes Pyrogen entdeckt, induziert IL-1 beta die Prostaglandinsynthese, den Zustrom und die Aktivierung von Neutrophilen, die Aktivierung von T-Zellen, die Produktion von Zytokinen, die Aktivierung von B-Zellen, die Produktion von Antikörpern sowie die Proliferation von Fibroblasten und die Kollagenproduktion. IL-1 beta spielt eine Rolle bei der Angiogenese, indem es synergistisch mit TNF und IL6 die VEGF-Produktion anregt.

Anwendungsinformationen

Kommentare:

Information on standard material:
The standard might be recombinant protein or natural protein, that will depend on the specific kit. Moreover, the expression system is E.coli or yeast or mammal cell. There is 0.05% proclin 300 in the standard as preservative.

Information on reagents:
The stop solution used in the kit is sulfuric acid with concentration of 1 mol/L. And the wash solution is TBS. The standard diluent contains 0.02 % sodium azide, assay diluent A and assay diluent B contain 0.01% sodium azide. Some kits can contain is BSA in them.

Information on antibodies:
The provided antibodies and their host vary in different kits.

Probenmenge: 100 μL

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards,
2. Add 100μL standard or sample to each well. Incubate 1 hours at 37 °C,
3. Aspirate and add 100μL prepared Detection Reagent A. Incubate 1 hour at 37 °C,
4. Aspirate and wash 3 times,
5. Add 100μL prepared Detection Reagent B. Incubate 30 minutes at 37 °C,
6. Aspirate and wash 5 times,
7. Add 90μL Substrate Solution. Incubate 10-20 minutes at 37 °C,
8. Add 50μL Stop Solution. Read at 450nm immediately.

Aufbereitung der Reagenzien:

1. Bring all kit components and samples to room temperature (18-25 °C) before use. If the kit will not be used up in one time, please only take out strips and reagents for present experiment, and leave the remaining strips and reagents in required condition.
2. Standard - Reconstitute the Standard with 1.0 mL of Standard Diluent, keep for 10 minutes at room temperature, shake gently (not to foam). The concentration of the standard in the stock solution is 10,000pg/mL. Firstly dilute the stock solution to 1,000pg/mL and the diluted standard serves as the highest standard (1,000pg/mL). Then prepare 7 tubes containing 0.5 mL Standard Diluent and use the diluted standard to produce a double dilution series. Mix each tube thoroughly before the next transfer. Set up 7 points of diluted standard such as 1,000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL, and the last microcentrifuge tube with Standard Diluent is the blank as 0pg/mL.
3. Detection Reagent A and Detection Reagent B - If lyophilized reconstitute the Detection Reagent A with 150μL of Reagent Diluent, keep for 10 minutes at room temperature, shake gently (not to foam). Briefly spin or centrifuge the stock Detection A and Detection B before use. Dilute them to the working concentration 100-fold with Assay Diluent A and B, respectively.
4. Wash Solution - Dilute 20 mL of Wash Solution concentrate (30x) with 580 mL of deionized or distilled water to prepare 600 mL of Wash Solution (1x).
5. TMB substrate - Aspirate the needed dosage of the solution with sterilized tips and do not dump the residual solution into the vial again.

Note:

1. Making serial dilution in the wells directly is not permitted.
2. Prepare standards within 15 minutes before assay. Please do not dissolve the reagents at 37 °C directly.
3. Please carefully reconstitute Standards or working Detection Reagent A and B according to the instruction, and avoid foaming and mix gently until the crystals are completely dissolved. To minimize imprecision caused by pipetting, use small volumes and ensure that pipettors are calibrated. It is recommended to suck more than 10μL for one pipetting.
4. The reconstituted Standards, Detection Reagent A and Detection Reagent B can be used only once.
5. If crystals have formed in the Wash Solution concentrate (30x), warm to room temperature and mix gently until the crystals are completely dissolved.
6. Contaminated water or container for reagent preparation will influence the detection result.

Aufbereitung der Proben:

• It is recommended to use fresh samples without long storage, otherwise protein degradation and denaturationmay occur in these samples, leading to false results. Samples should therefore be stored for a short periodat 2 - 8 °C or aliquoted at -20 °C (≤1 month) or -80 °C (≤ 3 months). Repeated freeze-thawcycles should be avoided. Prior to assay, the frozen samples should be slowly thawed and centrifuged toremove precipitates.
• If the sample type is not specified in the instructions, a preliminary test is necessary to determinecompatibility with the kit.
• If a lysis buffer is used to prepare tissue homogenates or cell culture supernatant, there is a possibilityof causing a deviation due to the introduced chemical substance.The recommended dilution factor is for reference only.
• Please estimate the concentration of the samples before performing the test. If the values are not in therange of the standard curve, the optimal sample dilution for the particular experiment has to be determined.Samples should then be diluted with PBS (pH =7.0-7.2).

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level of target were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level of target were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV < 10%
Inter-Assay: CV < 12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Vorsichtsmaßnahmen: The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung:

1. For unopened kit: All reagents should be stored according to the labels on the vials. The Standard, Detection Reagent A, Detection Reagent B, and 96-well Strip Plate should be stored at -20 °C upon receipt, while the other reagents should be stored at 4 °C.
2. For opened kits: the remaining reagents must be stored according to the above storage conditions. In addition, please return the unused wells to the foil pouch containing the desiccant and seal the foil pouch with the zipper.

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Haltbarkeit: 6 months

Referenzen für IL-1 beta ELISA Kit (ABIN6574166)

Tian, Wang, Liu, Yang, Wu, Wei, Chen: "Preparation and Evaluation of the Fully Humanized Monoclonal Antibody GD-mAb Against Respiratory Syncytial Virus." in: Frontiers in cellular and infection microbiology, Vol. 9, pp. 275, (2019) (PubMed).

Lenkiewicz, Adamiak, Thapa, Bujko, Pedziwiatr, Abdel-Latif, Kucia, Ratajczak, Ratajczak: "The Nlrp3 Inflammasome Orchestrates Mobilization of Bone Marrow-Residing Stem Cells into Peripheral Blood." in: Stem cell reviews and reports, Vol. 15, Issue 3, pp. 391-403, (2019) (PubMed).

Picone, Sabatino, Ditta, Amato, San Biagio, Mulè, Giacomazza, Dispenza, Di Carlo: "Nose-to-brain delivery of insulin enhanced by a nanogel carrier." in: Journal of controlled release : official journal of the Controlled Release Society, Vol. 270, pp. 23-36, (2019) (PubMed).

Zhou, Hu, Li, Yan, Ao, Yu, Fang, Liu, Li: "Levo-corydalmine alleviates vincristine-induced neuropathic pain in mice by inhibiting an NF-kappa B-dependent CXCL1/CXCR2 signaling pathway." in: Neuropharmacology, Vol. 135, pp. 34-47, (2019) (PubMed).

Zhang, Zhang, Wu, Xu, Yin, Zhang, Huang, Li: "Chronic glucocorticoid exposure activates BK-NLRP1 signal involving in hippocampal neuron damage." in: Journal of neuroinflammation, Vol. 14, Issue 1, pp. 139, (2018) (PubMed).

Zizzo, Frinchi, Nuzzo, Jinnah, Mudò, Condorelli, Caciagli, Ciccarelli, Di Iorio, Mulè, Belluardo, Serio: "Altered gastrointestinal motility in an animal model of Lesch-Nyhan disease." in: Autonomic neuroscience : basic & clinical, Vol. 210, pp. 55-64, (2018) (PubMed).

Lepore, DAlessandro, Antonangeli, Santoro, Esposito, Limatola, Trettel: "CXCL16/CXCR6 Axis Drives Microglia/Macrophages Phenotype in Physiological Conditions and Plays a Crucial Role in Glioma." in: Frontiers in immunology, Vol. 9, pp. 2750, (2018) (PubMed).

Chen, Chen, Liu, Wu, Yu, Qi, Wang, Yao, Huang, Han, Hou: "Epigallocatechingallate attenuates myocardial injury in a mouse model of heart failure through TGF‑β1/Smad3 signaling pathway." in: Molecular medicine reports, Vol. 17, Issue 6, pp. 7652-7660, (2018) (PubMed).

Qian, Xin, Lv, Wang, Zuo, Huang, Li, Xin: "Asiatic acid suppresses neuroinflammation in BV2 microglia via modulation of the Sirt1/NF-κB signaling pathway." in: Food & function, Vol. 9, Issue 2, pp. 1048-1057, (2018) (PubMed).

Wang, Jia, Shen, Wang, Fu, Su, Cai, Wang, Xiang: "Cathepsin B aggravates coxsackievirus B3-induced myocarditis through activating the inflammasome and promoting pyroptosis." in: PLoS pathogens, Vol. 14, Issue 1, pp. e1006872, (2018) (PubMed).

Guo, Zhang, Xia, Hou, Wang, Yang, Wang, Zhang, Chen, Wu: "Interleukin-1β induces intercellular adhesion molecule-1 expression, thus enhancing the adhesion between mesenchymal stem cells and endothelial progenitor cells via the p38 MAPK signaling pathway." in: International journal of molecular medicine, Vol. 41, Issue 4, pp. 1976-1982, (2018) (PubMed).

Liu, Tong, Zhang, Cong, Shi, Liu, Shi, Tong, Jin, Hou: "Chitosan oligosaccharide ameliorates acute lung injury induced by blast injury through the DDAH1/ADMA pathway." in: PLoS ONE, Vol. 13, Issue 2, pp. e0192135, (2018) (PubMed).

Miteva, Pappritz, Sosnowski, El-Shafeey, Müller, Dong, Savvatis, Ringe, Tschöpe, Van Linthout: "Mesenchymal stromal cells inhibit NLRP3 inflammasome activation in a model of Coxsackievirus B3-induced inflammatory cardiomyopathy." in: Scientific reports, Vol. 8, Issue 1, pp. 2820, (2018) (PubMed).

Guo, Jiang, Jiang, Tian, Li: "Lipoxin A4 may attenuate the progression of obesity-related glomerulopathy by inhibiting NF-κB and ERK/p38 MAPK-dependent inflammation." in: Life sciences, Vol. 198, pp. 112-118, (2018) (PubMed).

Zhuang, Wang, Shi, Qiu, Zhang, Xu, Chen, Jiang, Dong, Qiao: "MCMV triggers ROS/NLRP3-associated inflammasome activation in the inner ear of mice and cultured spiral ganglion neurons, contributing to sensorineural hearing loss." in: International journal of molecular medicine, Vol. 41, Issue 6, pp. 3448-3456, (2018) (PubMed).

Pang, Peng, Zhang, Wang, Jia, Bi, Chen: "Bushenkangshuai Tablet Reduces Atherosclerotic Lesion by Improving Blood Lipids Metabolism and Inhibiting Inflammatory Response via TLR4 and NF-κB Signaling Pathway." in: Evidence-based complementary and alternative medicine : eCAM, Vol. 2018, pp. 1758383, (2018) (PubMed).

Li, Chen, Xu, Liu, Li, Liu, Wang, Sun, Sheng, Kong: "Alamandine attenuates sepsis-associated cardiac dysfunction via inhibiting MAPKs signaling pathways." in: Life sciences, Vol. 206, pp. 106-116, (2018) (PubMed).

Wang, Li, Deng, Liu, He: "Ursolic Acid Ameliorates Inflammation in Cerebral Ischemia and Reperfusion Injury Possibly via High Mobility Group Box 1/Toll-Like Receptor 4/NFκB Pathway." in: Frontiers in neurology, Vol. 9, pp. 253, (2018) (PubMed).

Yoshizaki, Brito, Silva, Lino-Dos-Santos-Franco, Frias, E Silva, Amato-Lourenço, Saldiva, de Fátima Lopes Calvo Tibério, Mauad, Macchione: "The effects of particulate matter on inflammation of respiratory system: Differences between male and female." in: The Science of the total environment, Vol. 586, pp. 284-295, (2017) (PubMed).

Zhang, Zhou, Ye, Li, Wu, Li, Li: "Autophagy maintains the integrity of endothelial barrier in LPS-induced lung injury." in: Journal of cellular physiology, (2017) (PubMed).

Details zu IL-1 beta

Target: IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))

Andere Bezeichnung: Interleukin 1 Beta (IL1b) (IL1B Produkte)

Synonyme: IL-1 ELISA Kit, IL1-BETA ELISA Kit, IL1F2 ELISA Kit, IL-1BETA ELISA Kit, IL1beta ELISA Kit, il1-b ELISA Kit, zgc:111873 ELISA Kit, IL-1B ELISA Kit, IL-1beta ELISA Kit, Il-1b ELISA Kit, IL1B ELISA Kit, IL-1 beta ELISA Kit, IL-1b ELISA Kit, interleukin 1 beta ELISA Kit, interleukin 1, beta ELISA Kit, IL1B ELISA Kit, il1b ELISA Kit, Il1b ELISA Kit

UniProt: P10749

Pathways: NF-kappaB Signalweg, Interferon-gamma Pathway, TLR Signalweg, Negative Regulation of Hormone Secretion, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Autophagie, Cancer Immune Checkpoints, Inflammasome