beta Actin ELISA Kit

Produktnummer ABIN6574273

Produktdetails beta Actin ELISA Kit

Target: beta Actin (ACTB) (Actin, beta (ACTB))

Reaktivität: Human, Ratte, Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 1.56 ng/mL - 100 ng/mL

Untere Nachweisgrenze: 1.56 ng/mL

Applikation: ELISA

Verwendungszweck: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of ACTb in tissue homogenates, cell lysates, cell culture supernates. Due to the 100% homology of the sequence among different species, the kit can be used to detect human, mouse and rat samples.

We offer validation data (WB) for the kit components. So you can be sure to order a reliable ELISA kit product composed of high quality reagents.

Proben: Cell Culture Supernatant, Cell Lysate, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Beta Actin (ACTB)

Kreuzreaktivität (Details): No significant cross-reactivity or interference between Actin Beta (ACTb) and analogues was observed.

Sensitivität: 0.55 ng/mL

Bestandteile:

• Pre-coated, ready to use 96-well strip plate, flat buttom
• Plate sealer for 96 wells
• Reference Standard
• Standard Diluent
• Detection Reagent A
• Detection Reagent B
• Assay Diluent A
• Assay Diluent B
• Reagent Diluent (if Detection Reagent is lyophilized)
• TMB Substrate
• Stop Solution
• Wash Buffer (30 x concentrate)
• Instruction manual

Anwendungsinformationen

Kommentare:

Information on standard material:
The standard might be recombinant protein or natural protein, that will depend on the specific kit. Moreover, the expression system is E.coli or yeast or mammal cell. There is 0.05% proclin 300 in the standard as preservative.

Information on reagents:
The stop solution used in the kit is sulfuric acid with concentration of 1 mol/L. And the wash solution is TBS. The standard diluent contains 0.02 % sodium azide, assay diluent A and assay diluent B contain 0.01% sodium azide. Some kits can contain is BSA in them.

Information on antibodies:
The provided antibodies and their host vary in different kits.

Probenmenge: 100 μL

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards,
2. Add 100μL standard or sample to each well. Incubate 1 hours at 37 °C,
3. Aspirate and add 100μL prepared Detection Reagent A. Incubate 1 hour at 37 °C,
4. Aspirate and wash 3 times,
5. Add 100μL prepared Detection Reagent B. Incubate 30 minutes at 37 °C,
6. Aspirate and wash 5 times,
7. Add 90μL Substrate Solution. Incubate 10-20 minutes at 37 °C,
8. Add 50μL Stop Solution. Read at 450nm immediately.

Aufbereitung der Reagenzien:

1. Bring all kit components and samples to room temperature (18-25 °C) before use. If the kit will not be used up in one time, please only take out strips and reagents for present experiment, and leave the remaining strips and reagents in required condition.
2. Standard - Reconstitute the Standard with 0.5 mL of Standard Diluent, keep for 10 minutes at room temperature, shake gently (not to foam). The concentration of the standard in the stock solution is 100 ng/mL. Prepare 7 tubes containing 0.25 mL Standard Diluent and produce a double dilution series. Mix each tube thoroughly before the next transfer. Set up 7 points of diluted standard such as 100 ng/mL, 50 ng/mL, 25 ng/mL, 12.5 ng/mL, 6.25 ng/mL, 3.12 ng/mL, 1.56 ng/mL, and the last microcentrifuge tube with Standard Diluent is the blank as 0 ng/mL.
3. Detection Reagent A and Detection Reagent B - If lyophilized reconstitute the Detection Reagent A with 150μL of Reagent Diluent, keep for 10 minutes at room temperature, shake gently (not to foam). Briefly spin or centrifuge the stock Detection A and Detection B before use. Dilute them to the working concentration 100-fold with Assay Diluent A and B, respectively.
4. Wash Solution - Dilute 20 mL of Wash Solution concentrate (30x) with 580 mL of deionized or distilled water to prepare 600 mL of Wash Solution (1x).
5. TMB substrate - Aspirate the needed dosage of the solution with sterilized tips and do not dump the residual solution into the vial again.

Note:

1. Making serial dilution in the wells directly is not permitted.
2. Prepare standards within 15 minutes before assay. Please do not dissolve the reagents at 37 °C directly.
3. Please carefully reconstitute Standards or working Detection Reagent A and B according to the instruction, and avoid foaming and mix gently until the crystals are completely dissolved. To minimize imprecision caused by pipetting, use small volumes and ensure that pipettors are calibrated. It is recommended to suck more than 10μL for one pipetting.
4. The reconstituted Standards, Detection Reagent A and Detection Reagent B can be used only once.
5. If crystals have formed in the Wash Solution concentrate (30x), warm to room temperature and mix gently until the crystals are completely dissolved.
6. Contaminated water or container for reagent preparation will influence the detection result.

Aufbereitung der Proben:

• It is recommended to use fresh samples without long storage, otherwise protein degradation and denaturationmay occur in these samples, leading to false results. Samples should therefore be stored for a short periodat 2 - 8 °C or aliquoted at -20 °C (≤1 month) or -80 °C (≤ 3 months). Repeated freeze-thawcycles should be avoided. Prior to assay, the frozen samples should be slowly thawed and centrifuged toremove precipitates.
• If the sample type is not specified in the instructions, a preliminary test is necessary to determinecompatibility with the kit.
• If a lysis buffer is used to prepare tissue homogenates or cell culture supernatant, there is a possibilityof causing a deviation due to the introduced chemical substance.The recommended dilution factor is for reference only.
• Please estimate the concentration of the samples before performing the test. If the values are not in therange of the standard curve, the optimal sample dilution for the particular experiment has to be determined.Samples should then be diluted with PBS (pH =7.0-7.2).

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level of target were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level of target were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV < 10%
Inter-Assay: CV < 12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Vorsichtsmaßnahmen: The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung:

1. For unopened kit: All reagents should be stored according to the labels on the vials. The Standard, Detection Reagent A, Detection Reagent B, and 96-well Strip Plate should be stored at -20 °C upon receipt, while the other reagents should be stored at 4 °C.
2. For opened kits: the remaining reagents must be stored according to the above storage conditions. In addition, please return the unused wells to the foil pouch containing the desiccant and seal the foil pouch with the zipper.

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Haltbarkeit: 6 months

Referenzen für beta Actin ELISA Kit (ABIN6574273)

Grodzik, Sawosz, Sawosz, Hotowy, Wierzbicki, Kutwin, Jaworski, Chwalibog: "Nano-nutrition of chicken embryos. The effect of in ovo administration of diamond nanoparticles and L-glutamine on molecular responses in chicken embryo pectoral muscles." in: International journal of molecular sciences, Vol. 14, Issue 11, pp. 23033-44, (2013) (PubMed).

Jeffreys, Wilson, Thein: "Hypervariable 'minisatellite' regions in human DNA." in: Nature, Vol. 314, Issue 6006, pp. 67-73, (1985) (PubMed).

Details zu beta Actin

Target: beta Actin (ACTB) (Actin, beta (ACTB))

Andere Bezeichnung: Beta Actin (ACTB) (ACTB Produkte)

Synonyme: BRWS1 ELISA Kit, PS1TP5BP1 ELISA Kit, ACTA1 ELISA Kit, Actx ELISA Kit, E430023M04Rik ELISA Kit, beta-actin ELISA Kit, ps1tp5bp1 ELISA Kit, act-2 ELISA Kit, actb ELISA Kit, B-actin ELISA Kit, LOC100174883 ELISA Kit, LOC100304816 ELISA Kit, A ELISA Kit, ACT5C ELISA Kit, Ac5C ELISA Kit, Act ELISA Kit, Act-5C ELISA Kit, Act5 ELISA Kit, Act5c ELISA Kit, Actin ELISA Kit, Actin/BAP47 ELISA Kit, Actin5C ELISA Kit, BAP47 ELISA Kit, Bap47 ELISA Kit, CG4027 ELISA Kit, Dmel\\CG4027 ELISA Kit, M32055 ELISA Kit, T11 ELISA Kit, act ELISA Kit, act 5C ELISA Kit, act5C ELISA Kit, actin ELISA Kit, actin5C ELISA Kit, anon-EST:fe2D2 ELISA Kit, beta-actin/Bap47 ELISA Kit, cyt5C ELISA Kit, l(1)G0009 ELISA Kit, l(1)G0010 ELISA Kit, l(1)G0025 ELISA Kit, l(1)G0079 ELISA Kit, l(1)G0117 ELISA Kit, l(1)G0177 ELISA Kit, l(1)G0245 ELISA Kit, l(1)G0330 ELISA Kit, l(1)G0420 ELISA Kit, l(1)G0486 ELISA Kit, Bact ELISA Kit, ACT-5 ELISA Kit, ACTB ELISA Kit, B-ACTZF ELISA Kit, actba ELISA Kit, bact ELISA Kit, bactin1 ELISA Kit, bactzf ELISA Kit, wu:fd18f05 ELISA Kit, A26C1B ELISA Kit, POTE2alpha ELISA Kit, POTEACTIN ELISA Kit, Beta-actin ELISA Kit, actin beta ELISA Kit, actin, beta ELISA Kit, cytoplasmic actin OlCA1 ELISA Kit, beta-actin ELISA Kit, Actin 5C ELISA Kit, actin gamma 1 ELISA Kit, actin, beta 1 ELISA Kit, actin, beta L homeolog ELISA Kit, beta actin ELISA Kit, POTE ankyrin domain family member F ELISA Kit, actin, beta like 2 ELISA Kit, actin, cytoplasmic 1 ELISA Kit, ACTB ELISA Kit, Actb ELISA Kit, actb ELISA Kit, LOC100174883 ELISA Kit, LOC100304816 ELISA Kit, Act5C ELISA Kit, ACTG1 ELISA Kit, actb1 ELISA Kit, actb.L ELISA Kit, LOC100136352 ELISA Kit, POTEF ELISA Kit, ACTBL2 ELISA Kit, LOC109073280 ELISA Kit

Pathways: Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction