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CD13 ELISA Kit

ANPEP Reaktivität: Human Colorimetric Sandwich ELISA 312 pg/mL - 20000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
Produktnummer ABIN6719723
  • Target Alle CD13 (ANPEP) ELISA Kits anzeigen
    CD13 (ANPEP) (Alanyl (Membrane) Aminopeptidase (ANPEP))
    Reaktivität
    • 5
    • 4
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    312 pg/mL - 20000 pg/mL
    Untere Nachweisgrenze
    312 pg/mL
    Applikation
    ELISA
    Verwendungszweck
    Sandwich ELISA for Quantitative Detection of Antigen
    Proben
    Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
    Analytische Methode
    Quantitative
    Produktmerkmale

    Synonyms: Alanyl (membrane) aminopeptidase, Alanyl aminopeptidase, Alanyl membrane aminopeptidase, Aminopeptidase M, Aminopeptidase N, AMPN_HUMAN, ANPEP, AP M, AP N, AP-M, AP-N, APN, CD 13, CD13, CD13 antigen, gp150, hAPN, Lap 1, Lap1, Microsomal aminopeptidase, Myeloid plasma membrane glycoprotein CD13, p150, PEPN

    Background: Alanine aminopeptidase, also known as ANPEP or CD13. is an enzyme that is used as a biomarker to detect damage to the kidneys, and that may be used to help diagnose certain kidney disorders. It is mapped to 15q26.1. Aminopeptidase N is located in the small-intestinal and renal microvillar membrane, and also in other plasma membranes. In the small intestine, Aminopeptidase N plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases, and it is also thought to be involved in the metabolism of regulatory peptides by diverse cell types, including small intestinal and renal tubular epithelial cells, macrophages, granulocytes, and synaptic membranes from the CNS. Petrovic et al showed that CD13 was required for endothelial cell invasion in response to bradykinin. Inhibition of CD13 abrogated internalization of bradykinin receptor B2 and reduced endothelial cell motility.

    Gene Name: ANPEP

    Production: Natural and recombinant human CD13. There is no detectable cross-reactivity with other relevant proteins.

    Standard: Expression system for standard: NSO, Immunogen sequence: K69-K967

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  • Applikationshinweise

    Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 20,000pg/mL, 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL human CD13 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human CD13 standard solution and each sample be measured in duplicate.

    Blood Product Anticoagulant: Heparin Sodium

    ELISA Dilution: 312pg/mL-20,000pg/mL

    Probenmenge
    100 μL
    Plattentyp
    Pre-coated
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    RT,4 °C,-20 °C
    Informationen zur Lagerung
    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
  • Target Alle CD13 (ANPEP) ELISA Kits anzeigen
    CD13 (ANPEP) (Alanyl (Membrane) Aminopeptidase (ANPEP))
    Andere Bezeichnung
    CD13 - Aminopeptidase N (ANPEP Produkte)
    Synonyme
    APN ELISA Kit, CD13 ELISA Kit, GP150 ELISA Kit, LAP1 ELISA Kit, P150 ELISA Kit, PEPN ELISA Kit, A2AP ELISA Kit, AAP ELISA Kit, ALPHA-2-PI ELISA Kit, API ELISA Kit, PLI ELISA Kit, AAT1 ELISA Kit, ALT1 ELISA Kit, GPT1 ELISA Kit, Gpt1 ELISA Kit, Apn ELISA Kit, pepN ELISA Kit, SC8E4A.13 ELISA Kit, 1300007J06Rik ELISA Kit, 2310022B03Rik ELISA Kit, ALT ELISA Kit, Gpt-1 ELISA Kit, AP-M ELISA Kit, AP-N ELISA Kit, Cd13 ELISA Kit, Apm ELISA Kit, KZP ELISA Kit, Lap1 ELISA Kit, rAPN ELISA Kit, APDE ELISA Kit, p150 ELISA Kit, anpep ELISA Kit, fb64c05 ELISA Kit, wu:fb64c05 ELISA Kit, zgc:136771 ELISA Kit, alanyl aminopeptidase, membrane ELISA Kit, serpin family F member 2 ELISA Kit, glutamic--pyruvic transaminase ELISA Kit, aminopeptidase N ELISA Kit, aminopeptidase ELISA Kit, aminopeptidase n ELISA Kit, glutamic pyruvic transaminase, soluble ELISA Kit, alanyl (membrane) aminopeptidase ELISA Kit, membrane alanyl aminopeptidase ELISA Kit, alanyl (membrane) aminopeptidase b ELISA Kit, alanyl aminopeptidase, membrane L homeolog ELISA Kit, ANPEP ELISA Kit, SERPINF2 ELISA Kit, GPT ELISA Kit, Gpt ELISA Kit, Apn2 ELISA Kit, Apn1 ELISA Kit, SCO2643 ELISA Kit, pepN ELISA Kit, Shewana3_0897 ELISA Kit, Alvin_1024 ELISA Kit, Ndas_3413 ELISA Kit, Saut_1544 ELISA Kit, Fbal_1877 ELISA Kit, ML5_3424 ELISA Kit, Despr_1988 ELISA Kit, Mesop_5365 ELISA Kit, Anpep ELISA Kit, LOC101744250 ELISA Kit, anpepb ELISA Kit, anpep.L ELISA Kit
    Gen-ID
    290
    NCBI Accession
    NP_001141
    UniProt
    P15144
    Pathways
    Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones
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