S100A12 ELISA Kit

Produktnummer ABIN6719725

Produktdetails S100A12 ELISA Kit

Target: S100A12 (S100 Calcium Binding Protein A12 (S100A12))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 312 pg/mL - 20000 pg/mL

Untere Nachweisgrenze: 312 pg/mL

Applikation: ELISA

Verwendungszweck: Sandwich ELISA for Quantitative Detection of Antigen

Proben: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytische Methode: Quantitative

Produktmerkmale:

Synonyms: CAAF1, CAGC, Calcitermin, Calcium binding protein in amniotic fluid, Calcium-binding protein in amniotic fluid 1, Calgranulin C, Calgranulin related protein, Calgranulin-C, CGRP, EN RAGE, EN-RAGE, ENRAGE, Extracellular newly identified RAGE-binding protein, migration inhibitory factor-related protein 6, MRP6, Neutrophil S100 protein, p6, Protein S100 A12, S100 calcium binding protein A12, S100 calcium-binding protein A12 (calgranulin C), S100 calcium-binding protein A12, S10AC_HUMAN

Background: S100 calcium-binding protein A12 (S100A12) is a protein that in humans is encoded by the S100A12 gene. It is also known as calgranulin C. By direct R-banding FISH, the S100A12 gene is mapped to chromosome 1q21.2-q22. The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. And this protein is proposed to be involved in specific calcium-dependent signal transduction pathways and its regulatory effect on cytoskeletal components may modulate various neutrophil activities.

Gene Name: S100A12

Production: Natural and recombinant human S100A12. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: E.coli, Immunogen sequence: M1-E92

Anwendungsinformationen

Applikationshinweise:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 20,000pg/mL, 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL human S100A12 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma( heparin, EDTA) to each empty well. It is recommended that each human S100A12 standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 312pg/mL-20,000pg/mL

Other Performance Data: DOE, 2-8° C - 04/19/19 -20° C - 10/18/19

Probenmenge: 100 μL

Plattentyp: Pre-coated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: RT,4 °C,-20 °C

Informationen zur Lagerung: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Details zu S100A12

Target: S100A12 (S100 Calcium Binding Protein A12 (S100A12))

Andere Bezeichnung: S100A12 (S100A12 Produkte)

Synonyme: CAAF1 ELISA Kit, CAGC ELISA Kit, CGRP ELISA Kit, ENRAGE ELISA Kit, MRP-6 ELISA Kit, MRP6 ELISA Kit, p6 ELISA Kit, CO-AG ELISA Kit, EN-RAGE ELISA Kit, S100 calcium binding protein A12 ELISA Kit, protein S100-A12 ELISA Kit, S100A12 ELISA Kit, LOC101103771 ELISA Kit

Gen-ID: 6283

NCBI Accession: NP_005612

UniProt: P80511

Pathways: Toll-Like Receptors Cascades, S100 Proteine