CXCL5 ELISA Kit

Produktnummer ABIN6719747

Produktdetails CXCL5 ELISA Kit

Target: CXCL5 (Chemokine (C-X-C Motif) Ligand 5 (CXCL5))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 31.2 pg/mL - 2000 pg/mL

Untere Nachweisgrenze: 31.2 pg/mL

Applikation: ELISA

Verwendungszweck: Sandwich ELISA for Quantitative Detection of Antigen

Proben: Cell Culture Supernatant, Plasma (EDTA - heparin - citrate), Serum

Analytische Methode: Quantitative

Produktmerkmale:

Synonyms: AMCF II, AMCFII, C X C motif chemokine 5, Chemokine (C X C motif) ligand 5, CXCL 5, ENA 78, ENA78, Epithelial derived neutrophil activating protein 78, GCP 2, LIX|Neutrophil activating peptide ENA 78, Neutrophil activating protein 78, SCYB5, Scyb6, Small inducible cytokine B5, Small inducible cytokine subfamily B (Cys X Cys) member 5 (epithelial derived neutrophil activating peptide 78), Small inducible cytokine subfamily B, member 15

Background: C-X-C motif chemokine 5 is a protein that in humans encoded by the CXCL5 gene. The protein encoded by this gene, CXCL5 is a small cytokine belonging to the CXC chemokine family that is also known as epithelial-derived neutrophil-activating peptide 78(ENA-78). It is produced following stimulation of cells with the inflammatory cytokines interleukin-1or tumor necrosis factor-alpha. Expression of CXCL5 has also been observed in eosinophils, and can be inhibited with the type II interferon IFN-gamma. This chemokine stimulates the chemotaxis of neutrophils possessing angiogenic properties. It elicits these effects by interacting with the cell surface chemokine receptor CXCR2. The gene for CXCL5 is encoded on four exons and is located on humanchromosome 4 amongst several other CXC chemokine genes. CXCL5 has been implicated in connective tissue remodeling. CXCL5 plays a role in reducing sensitivity to sunburn pain in some subjects, and is a potential target which can be utilized to understand more about pain in other inflammatory conditions like arthritis and cystitis.

Gene Name: CXCL5

Production: Natural and recombinant human CXCL5. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: E.coli, Immunogen sequence: A37-N114

Anwendungsinformationen

Applikationshinweise:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human CXCL5 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA, citrate) to each empty well. It is recommended that each human CXCL5 standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 31.2pg/mL-2000pg/mL

Probenmenge: 100 μL

Plattentyp: Pre-coated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: RT,4 °C,-20 °C

Informationen zur Lagerung: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Details zu CXCL5

Target: CXCL5 (Chemokine (C-X-C Motif) Ligand 5 (CXCL5))

Andere Bezeichnung: CXCL5 - ENA-78 (CXCL5 Produkte)

Synonyme: ENA-78 ELISA Kit, SCYB5 ELISA Kit, Cxcl6 ELISA Kit, CXCL5 ELISA Kit, GCP-2 ELISA Kit, GCP2 ELISA Kit, SCYB6 ELISA Kit, AMCF-II ELISA Kit, LIX ELISA Kit, Scyb5 ELISA Kit, Scyb6 ELISA Kit, C-X-C motif chemokine ligand 5 ELISA Kit, C-X-C motif chemokine ligand 6 ELISA Kit, chemokine (C-X-C motif) ligand 5 ELISA Kit, C-X-C motif chemokine 5 ELISA Kit, CXCL5 ELISA Kit, Cxcl6 ELISA Kit, LOC701946 ELISA Kit, LOC703222 ELISA Kit, Cxcl5 ELISA Kit

Gen-ID: 6374

NCBI Accession: NP_002985

UniProt: P42830

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity