G-CSF ELISA Kit

Produktnummer ABIN6719749

Produktdetails G-CSF ELISA Kit

Target: G-CSF (CSF3) (Colony Stimulating Factor 3 (Granulocyte) (CSF3))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 31.2 pg/mL - 2000 pg/mL

Untere Nachweisgrenze: 31.2 pg/mL

Applikation: ELISA

Verwendungszweck: Sandwich ELISA for Quantitative Detection of Antigen

Proben: Cell Culture Supernatant, Plasma (EDTA - heparin - citrate), Serum

Analytische Methode: Quantitative

Produktmerkmale:

Synonyms: C17orf33, Colony stimulating factor 3 (granulocyte), CSF 3, CSF beta, CSF3, CSF3OS, Csfg, Filgrastim, GCSA, GCSF, Granulocyte colony stimulating fact, Lenograstim, Macrophage granulocyte inducer 2, MGC45931, MGI 2, MGI IG, OTTMUSP00000006785, Pluripoietin, RP23-395E10.4

Background: Granulocyte colony-stimulating factor(G-CSF) is a member of the CSF family of hormone-like glycoprotein that regulates hematopoietic cell proliferation and differentiation, and it almost exclusively stimulates the colony formation of granulocytes from committed precursor cells in semi-solid agar culture. G-CSF is also termed colony stimulating factor-3, and a single gene of which codes for a 177 or 180 amino acid mature protein of molecular weight 19,600. Functionally, it specifically stimulates the proliferation and differentiation of the progenitor cells for granulocytes. The effect of G-CSF on myeloid leukemias is unique among colony stimulating factors in driving the leukemic cells from a self-renewing malignant state to a mature differentiated phenotype with the concomitant loss of tumorigenicity. Besides, it also prevents cardiac remodeling after myocardial infarction by activating the Jak-Stat pathway in cardiomyocytes. The recombinant form of hG-CSF is capable of supporting neutrophil proliferation in a CFU-GM assay as well as early erythroid colonies and mixed colony formation. Human gene coding for G-CSF is assigned to the q21-q22 region of chromosome 17. The standard product used in this kit is recombinant human G-CSF, consisting of 175 amino acids with the molecular mass of 18.8KDa.

Gene Name: CSF3

Production: Natural and recombinant human G-CSF. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: E.coli, Immunogen sequence: T31-P204

Anwendungsinformationen

Applikationshinweise:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human G-CSF standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA, citrate) to each empty well. It is recommended that each human G-CSF standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 31.2pg/mL-2000pg/mL

Probenmenge: 100 μL

Plattentyp: Pre-coated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: RT,4 °C,-20 °C

Informationen zur Lagerung: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Details zu G-CSF

Target: G-CSF (CSF3) (Colony Stimulating Factor 3 (Granulocyte) (CSF3))

Andere Bezeichnung: G-CSF (CSF3 Produkte)

Synonyme: CSF3 ELISA Kit, G-CSF ELISA Kit, gcsf ELISA Kit, Csfg ELISA Kit, MGI-IG ELISA Kit, C17orf33 ELISA Kit, CSF3OS ELISA Kit, GCSF ELISA Kit, Gcsf ELISA Kit, colony stimulating factor 3 ELISA Kit, colony stimulating factor 3 (granulocyte) a ELISA Kit, colony stimulating factor 3 (granulocyte) ELISA Kit, CSF3 ELISA Kit, csf3a ELISA Kit, Csf3 ELISA Kit

Gen-ID: 1440

NCBI Accession: NP_000750

UniProt: P09919

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization