IL6RA ELISA Kit
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- Target Alle IL6RA ELISA Kits anzeigen
- IL6RA (Interleukin 6 Receptor, alpha (IL6RA))
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Reaktivität
- Human
- Nachweismethode
- Colorimetric
- Methodentyp
- Sandwich ELISA
- Detektionsbereich
- 31.2 pg/mL - 2000 pg/mL
- Untere Nachweisgrenze
- 31.2 pg/mL
- Applikation
- ELISA
- Hersteller Produkt- Nr.
- koa0458
- Hersteller
- Rockland
- Verwendungszweck
- Human IL-6R alpha Sandwich ELISA Kit for Quantitative Detection
- Marke
- AccuSignal™
- Proben
- Cell Culture Supernatant, Plasma (EDTA - heparin - citrate), Serum
- Analytische Methode
- Quantitative
- Spezifität
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Production: Natural and recombinant human IL-6R alpha. There is no detectable cross-reactivity with other relevant proteins.
- Sensitivität
- < 10 pg/mL
- Bestandteile
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- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
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- Applikationshinweise
- Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human IL-6R alpha standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA, citrate) or urine to each empty well. It is recommended that each human IL-6R alpha standard solution and each sample be measured in duplicate.
- Kommentare
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Standard: Expression system for standard: sf21, Immunogen sequence: L20-D358
- Probenmenge
- 100 μL
- Plattentyp
- Pre-coated
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Lagerung
- 4 °C,-20 °C
- Informationen zur Lagerung
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
- Haltbarkeit
- 12 months
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- Target Alle IL6RA ELISA Kits anzeigen
- IL6RA (Interleukin 6 Receptor, alpha (IL6RA))
- Andere Bezeichnung
- IL-6R alpha (IL6RA Produkte)
- Hintergrund
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Synonyms: CD126, CD126 antigen, Gp80, IL 6R 1, IL 6R alpha, IL-6 receptor subunit alpha, IL-6R 1, IL-6R subunit alpha, IL-6R-1, IL-6R-alpha, IL-6RA, Il6r, IL6R1, IL6RA, IL6RA_HUMAN, IL6Ralpha, Interleukin 6 receptor, Interleukin 6 receptor alpha chain, Interleukin-6 receptor subunit alpha, Membrane glycoprotein 80, MGC104991, OTTHUMP00000034320
Background: IL6R alpha(IL6RA) also known as CD126 or IL6R, is a type I cytokine receptor. The IL6 receptor is a protein complex consisting of a IL-6 receptor subunit(IL6R) and interleukin 6 signal transducer Glycoprotein 130. IL6R also denotes the human gene encoding this subunit. Alternatively spliced transcript variants encoding distinct isoforms have been reported. IL6R subunit is also shared by many other cytokines. Interleukin-6 receptor has been shown to interact with Interleukin 6 and Ciliary neurotrophic factor. Ligand binding did not appear to affect IL6R dimerization status. IL6R dimerization occurs both on the cell surface and in solution.
- Gen-ID
- 3570
- NCBI Accession
- NP_000556
- UniProt
- A0N0L5
- Pathways
- JAK-STAT Signalweg, Autophagie, Growth Factor Binding, Cancer Immune Checkpoints
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