FOLR1 ELISA Kit

Produktnummer ABIN6719802

Hersteller Produkt- Nr.: koa0731

Produktdetails FOLR1 ELISA Kit

Target: FOLR1 (Folate Receptor 1 (Adult) (FOLR1))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 46.9 pg/mL - 30000 pg/mL

Untere Nachweisgrenze: 46.9 pg/mL

Applikation: ELISA

Hersteller Produkt- Nr.: koa0731

Hersteller: Rockland

Verwendungszweck: Human FOLR1 Sandwich ELISA Kit for Quantitative Detection

Marke: AccuSignal™

Proben: Breast Milk, Cell Culture Supernatant, Plasma (EDTA - heparin), Saliva, Serum, Urine

Analytische Methode: Quantitative

Spezifität:

Production: Natural and recombinant human FOLR1. There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: < 10 pg/mL

Bestandteile:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Anwendungsinformationen

Applikationshinweise: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 3000pg/mL, 1500pg/mL, 750pg/mL, 419pg/mL, 188pg/mL, 94pg/mL, 46.9pg/mL human FOLR1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA), saliva, urine or human milk to each empty well. We recommend that each human FOLR1 standard solution and each sample is measured in duplicate.

Kommentare:

Standard: Expression system for standard: E.coli, Immunogen sequence: R25-S234

Probenmenge: 100 μL

Plattentyp: Pre-coated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Haltbarkeit: 12 months

Details zu FOLR1

Target: FOLR1 (Folate Receptor 1 (Adult) (FOLR1))

Andere Bezeichnung: FOLR1 (FOLR1 Produkte)

Hintergrund:

Synonyms: Adult folate binding protein, Adult folate-binding protein, FBP, Folate Receptor 1 Adult, Folate receptor 1, Folate Receptor 1 Precursor, Folate receptor adult, Folate receptor alpha, Folate receptor, FOLR, FOLR1, FOLR1_HUMAN, FR alpha, FR-alpha, FRalpha, KB cells FBP, MOV18, Ovarian cancer associated antigen, Ovarian tumor associated antigen, Ovarian tumor associated antigen MOv18, Ovarian tumor-associated antigen MOv18

Background: Folate receptor 1 adult is a protein that in humans is encoded by the FOLR1 gene. The protein encoded by this gene is a member of the folate receptor(FOLR) family. It is mapped to 11q13.4. FOLR1 expression in Jurkat cells facilitated MBG or EBO entry, and FR-blocking reagents inhibited infection of MBG or EBO. This gene product is a secreted protein that either anchors to membranes via a glycosyl-phosphatidylinositol linkage or exists in a soluble form. The FOLR1 gene encodes the adult folate receptor, or folate-binding protein(FBP), which has a high affinity for folic acid and for several reduced folic acid derivatives, and mediates delivery of 5-methyltetrahydrofolate to the interior of cells. FOLR1 is also an important regulator of milk protein synthesis.

Gen-ID: 2348

NCBI Accession: NP_000793

UniProt: P15328

Pathways: Dicarboxylic Acid Transport