PD-1 ELISA Kit

Produktnummer ABIN6719831

Produktdetails PD-1 ELISA Kit

Target: PD-1 (PDCD1) (Programmed Cell Death 1 (PDCD1))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 156 pg/mL - 10000 pg/mL

Untere Nachweisgrenze: 156 pg/mL

Applikation: ELISA

Verwendungszweck: Sandwich ELISA for Quantitative Detection of Antigen

Proben: Cell Culture Supernatant

Analytische Methode: Quantitative

Produktmerkmale:

Synonyms: CD279, CD279 antigen, hPD 1, hPD-1, PD 1, PD-1, PD1, PDCD 1, PDCD1, PDCD1_HUMAN, Programmed cell death 1, Programmed cell death protein 1, Protein PD 1, Protein PD-1, SLEB2

Background: Programmed cell death protein 1, also called PD-1 or PDCD1 is a protein that in humans is encoded by the PDCD1 gene. This gene encodes a cell surface membrane protein of the immunoglobulin superfamily. By fluorescence in situ hybridization, the PDCD1 gene is mapped to 2q37.3. This protein is expressed in pro-B-cells and is thought to play a role in their differentiation. In mice, expression of this gene is induced in the thymus when anti-CD3 antibodies are injected and large numbers of thymocytes undergo apoptosis. Mice deficient for this gene bred on a BALB/c background developed dilated cardiomyopathy and died from congestive heart failure. These studies suggest that this gene product may also be important in T cell function and contribute to the prevention of autoimmune diseases.

Gene Name: PDCD1

Production: Natural and recombinant human PD-1. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: NSO, Immunogen sequence: L25-Q167

Anwendungsinformationen

Applikationshinweise:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human PD-1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates to each empty well. It is recommended that each human PD-1 standard solution and each sample be measured in duplicate.

ELISA Dilution: 156pg/mL-10000pg/mL

Probenmenge: 100 μL

Plattentyp: Pre-coated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: RT,4 °C,-20 °C

Informationen zur Lagerung: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Details zu PD-1

Target: PD-1 (PDCD1) (Programmed Cell Death 1 (PDCD1))

Andere Bezeichnung: PD-1 (PDCD1 Produkte)

Synonyme: CD279 ELISA Kit, PD-1 ELISA Kit, PD1 ELISA Kit, SLEB2 ELISA Kit, hPD-1 ELISA Kit, hPD-l ELISA Kit, Ly101 ELISA Kit, Pdc1 ELISA Kit, programmed cell death 1 ELISA Kit, PDCD1 ELISA Kit, Pdcd1 ELISA Kit

Gen-ID: 5133

NCBI Accession: NP_005009

UniProt: Q15116

Pathways: Cancer Immune Checkpoints