P-Cadherin ELISA Kit

Produktnummer ABIN6719852

Produktdetails P-Cadherin ELISA Kit

Target: P-Cadherin (CDH3)

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 62.5 pg/mL - 4000 pg/mL

Untere Nachweisgrenze: 62.5 pg/mL

Applikation: ELISA

Verwendungszweck: Sandwich ELISA for Quantitative Detection of Antigen

Proben: Cell Culture Supernatant, Serum

Analytische Methode: Quantitative

Produktmerkmale:

Synonyms: AI385538, CADH3_HUMAN, Cadherin 3, Cadherin 3 precursor, Cadherin 3 type 1, Cadherin-3, Cadp, Calcium dependent adhesion protein placental, CDH 3, CDH3, CDH3 protein, CDHP, HJMD, P cadherin (placental), P-cadherin, PCAD, Placental cadherin

Background: Cadherins are calcium-dependent cell-cell adhesion molecules that mediate cell-cell binding in a homophilic manner. They play an important role in the growth and development of cells via the mechanisms of control of tissue architecture and the maintenance of tissue integrity. Cadherin expression is regulated spatially as well as temporally. Cadherins are thought to play an important role in development and maintenance of tissues through selective cell-cell adhesion activity and may be involved also in the invasion and metastasis of malignant tumors. Cadherin regulates dendritic spine morphogenesis. A cadherin gene cluster is mapped to a region of chromosome 5 subject to frequent allelic loss in carcinoma. The standard product used in this kit is recombinant P-Cadherin with the molecular mass of 120-130Kda after glycosylation.

Gene Name: CDH3

Production: Natural and recombinant human P-Cadherin. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: NSO, Immunogen sequence: D108-G654

Anwendungsinformationen

Applikationshinweise:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human P-Cadherin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates or serum to each empty well. It is recommended that each human P-Cadherin standard solution and each sample be measured in duplicate.

ELISA Dilution: 62.5pg/mL-4000pg/mL

Probenmenge: 100 μL

Plattentyp: Pre-coated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: RT,4 °C,-20 °C

Informationen zur Lagerung: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Details zu P-Cadherin

Target: P-Cadherin (CDH3)

Andere Bezeichnung: P-Cadherin (CDH3 Produkte)

Synonyme: CDH3 ELISA Kit, Cadherin-3 ELISA Kit, AI385538 ELISA Kit, Cadp ELISA Kit, Cdhp ELISA Kit, P-cadherin ELISA Kit, Pcad ELISA Kit, CDHP ELISA Kit, HJMD ELISA Kit, PCAD ELISA Kit, E-Cad ELISA Kit, E-cadherin ELISA Kit, EP-cadherin ELISA Kit, XTCAD-1 ELISA Kit, c-cadherin ELISA Kit, cadherin-1 ELISA Kit, cdh1 ELISA Kit, cdh1-b ELISA Kit, cdh3 ELISA Kit, cdhc-A ELISA Kit, cdhp ELISA Kit, hjmd ELISA Kit, l-cam ELISA Kit, lcam ELISA Kit, pcad ELISA Kit, uvo ELISA Kit, uvomorulin ELISA Kit, xb-cad ELISA Kit, xcdh1 ELISA Kit, CDH15 ELISA Kit, K-CAM ELISA Kit, cadherin 3 ELISA Kit, cadherin 3 L homeolog ELISA Kit, Cadherin-3 ELISA Kit, CDH3 ELISA Kit, Cdh3 ELISA Kit, cdh3.L ELISA Kit, cdh-3 ELISA Kit

Gen-ID: 1001

NCBI Accession: NP_001304124

UniProt: P22223