IGFBP3 ELISA Kit

Produktnummer ABIN6719869

Hersteller Produkt- Nr.: koa0204

Produktdetails IGFBP3 ELISA Kit

Target: IGFBP3 (Insulin-Like Growth Factor Binding Protein 3 (IGFBP3))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 156 pg/mL - 10000 pg/mL

Untere Nachweisgrenze: 156 pg/mL

Applikation: ELISA

Hersteller Produkt- Nr.: koa0204

Hersteller: Rockland

Verwendungszweck: Mouse IGFBP-3 Sandwich ELISA Kit for Quantitative Detection

Marke: AccuSignal™

Proben: Cell Culture Supernatant, Serum

Analytische Methode: Quantitative

Spezifität:

Production: Natural and recombinant mouse IGFBP-3. There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: < 10 pg/mL

Bestandteile:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Anwendungsinformationen

Applikationshinweise: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 313pg/mL, 156pg/mL mouse IGFBP-3 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates or serum to each empty well. It is recommended that each mouse IGFBP-3 standard solution and each sample be measured in duplicate.

Kommentare:

Standard: Expression system for standard: NSO, Immunogen sequence: G28Q-K291

Probenmenge: 100 μL

Plattentyp: Pre-coated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Haltbarkeit: 12 months

Details zu IGFBP3

Target: IGFBP3 (Insulin-Like Growth Factor Binding Protein 3 (IGFBP3))

Andere Bezeichnung: IGFBP-3 (IGFBP3 Produkte)

Hintergrund:

Synonyms: Acid stable subunit of the 140 K IGF complex, Binding protein 29, Binding protein 53, BP 53, BP53, Growth hormone dependent binding protein, IBP 3, IBP3, IGF binding protein 3, IGFBP 3, Insulin Like Growth Factor Binding Protein 3

Background: Insulin-like growth factor(IGF)-binding protein-3(IGFBP-3) is a major determinant of circulating levels of the IGFs and is clinically useful for the evaluation of GH deficiency and for predicting the response to GH treatment. The circulating level of IGFBP-3 is inversely related to the risk of several common cancers, and that antiproliferative agents such as antiestrogens and retinoids act in part by up-regulating IGFBP-3 gene(IGFBP3) expression. Insulin-like growth factor-binding protein(IGFBP)-3, well characterized as the carrier of insulin-like growth factor(IGF), has been reported to have intrinsic bioactivity that is independent of IGF binding. IGFBP-3 has an IGF-independent, antiproliferative effect in undifferentiated and early differentiated but not in terminally differentiated chondrocytes. IGFBP-3 possesses both growth-inhibitory and potentiating effects on cells that are independent of IGF action and are mediated through specific. IGFBP-3 binding proteins/receptors locate at the cell membrane, cytosol, or nuclear compartments and in the extrIGFBP-3llular matrix. IGFBP3 is also located on chromosome 7. The standard product used in this kit is recombinant mouse IGFBP-3, consisting of 270 amino acids with the molecular mass of 29.4 kDa. As a result of glycosylation, the molecular mass is 40-50KDa.

Gen-ID: 3486

NCBI Accession: NP_000589

UniProt: B3KPF0

Pathways: Myometrial Relaxation and Contraction, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Autophagie, Smooth Muscle Cell Migration, Growth Factor Binding