Nidogen 1 ELISA Kit

Produktnummer ABIN6719872

Produktdetails Nidogen 1 ELISA Kit

Target: Nidogen 1 (NID1)

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 62.5 pg/mL - 4000 pg/mL

Untere Nachweisgrenze: 62.5 pg/mL

Applikation: ELISA

Verwendungszweck: Sandwich ELISA for Quantitative Detection of Antigen

Proben: Cell Culture Supernatant, Serum

Analytische Methode: Quantitative

Produktmerkmale:

Synonyms: Entactin, NID-1, Nid1, NID1_HUMAN, Nidogen-1

Background: Nidogen-1(NID-1), also known as Entactin, is a protein that in humans is encoded by the NID1 gene. It is a member of the nidogen family of basement membrane glycoproteins. This gene is mapped to 1q42.3. Nidogen-1 is a component of the basement membrane alongside other components such as collagen type IV, proteoglycans(heparan sulfate and glycosaminoglycans), laminin and fibronectin. The protein interacts with several other components of basement membranes. Structurally it(along with perlecan) connects the networks formed by collagens and laminins to each other. It may also play a role in cell interactions with the extracellular matrix. Nidogen-1 also can serve as a bridge between the 2 most abundant molecules in the basement membrane: type IV collagen and laminin.

Gene Name: NID1

Production: Natural and recombinant human Nidogen-1. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: NSO, Immunogen sequence: L29-K1114

Anwendungsinformationen

Applikationshinweise:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human Nidogen-1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates or serum to each empty well. It is recommended that each human Nidogen-1 standard solution and each sample be measured in duplicate.

ELISA Dilution: 62.5pg/mL-4000pg/mL

Probenmenge: 100 μL

Plattentyp: Pre-coated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: RT,4 °C,-20 °C

Informationen zur Lagerung: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Details zu Nidogen 1

Target: Nidogen 1 (NID1)

Andere Bezeichnung: Entactin - NID-1 (NID1 Produkte)

Synonyme: NID1 ELISA Kit, nid1 ELISA Kit, im:7055206 ELISA Kit, im:7142300 ELISA Kit, mg:bb01h02 ELISA Kit, LOC100219409 ELISA Kit, A630025O17 ELISA Kit, Nid ELISA Kit, entactin ELISA Kit, entactin-1 ELISA Kit, nidogen-1 ELISA Kit, NID ELISA Kit, nidogen 1 ELISA Kit, nidogen 1a ELISA Kit, NID1 ELISA Kit, nid1a ELISA Kit, nid1 ELISA Kit, Nid1 ELISA Kit

Gen-ID: 4811

NCBI Accession: NP_002499

UniProt: P14543