Biglycan ELISA Kit

Produktnummer ABIN6719885

Produktdetails Biglycan ELISA Kit

Target: Biglycan (BGN)

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 156 pg/mL - 10000 pg/mL

Untere Nachweisgrenze: 156 pg/mL

Applikation: ELISA

Verwendungszweck: Sandwich ELISA for Quantitative Detection of Antigen

Proben: Cell Culture Lysate, Cell Culture Supernatant, Plasma (EDTA - heparin), Serum, Tissue Homogenate

Analytische Methode: Quantitative

Produktmerkmale:

Synonyms: BGN, Biglycan, Biglycan proteoglycan, Bone/cartilage proteoglycan I, Dermatan sulphate proteoglycan I, DSPG1, PG S1, PG-S1, PGI, PGS1_HUMAN, SLRR1A, Small leucine rich protein 1A

Background: Biglycan (BGN) is a small leucine-rich repeat proteoglycan (SLRP) which is found in a variety of extracellular matrix tissues, including bone, cartilage and tendon. It is an important structural component of articular cartilage and participates in the assembly of the chondrocyte extracellular matrix through formation of protein interactions with type VI collagen and large proteoglycan aggregates. In humans, Biglycan is encoded by the BGN gene. It is mapped to Xq28. Biglycan is believed to play a role in the mineralisation of bone, and its core protein binds to the growth factors BMP-4 and influences its bioactivity. It has been reported that the presence of biglycan is necessary for BMP-4 to exert its effects on osteoblasts. There is also evidence that biglycan can bind to TGF-beta 1. In addition, Biglycan plays an important role in cell signaling.

Gene Name: BGN

Production: Natural and recombinant human Biglycan. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: NSO, Immunogen sequence: D38-K368

Anwendungsinformationen

Applikationshinweise:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human Biglycan standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA) or tissue homogenates to each empty well. It is recommended that each human Biglycan standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 156pg/mL-10,000pg/mL

Other Performance Data: DOE at 4 °C 18JAN19. DOE at -20 °C 19JUL19.

Probenmenge: 100 μL

Plattentyp: Pre-coated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: RT,4 °C,-20 °C

Informationen zur Lagerung: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Details zu Biglycan

Target: Biglycan (BGN)

Andere Bezeichnung: Biglycan (BGN Produkte)

Synonyme: DSPG1 ELISA Kit, PG-S1 ELISA Kit, PGI ELISA Kit, SLRR1A ELISA Kit, BG ELISA Kit, BSPG1 ELISA Kit, bgn ELISA Kit, zgc:91989 ELISA Kit, dspg1 ELISA Kit, pg-s1 ELISA Kit, slrr1a ELISA Kit, xbgn ELISA Kit, BGN ELISA Kit, zgc:77123 ELISA Kit, DKFZp468I2427 ELISA Kit, biglycan ELISA Kit, biglycan L homeolog ELISA Kit, biglycan a ELISA Kit, biglycan S homeolog ELISA Kit, biglycan b ELISA Kit, BGN ELISA Kit, Bgn ELISA Kit, bgn.L ELISA Kit, bgna ELISA Kit, bgn.S ELISA Kit, bgn ELISA Kit, bgnb ELISA Kit

Gen-ID: 633

NCBI Accession: NP_001702

UniProt: P21810

Pathways: Glycosaminoglycan Metabolic Process