CD5L ELISA Kit

Produktnummer ABIN6719889

Hersteller Produkt- Nr.: koa0853

Produktdetails CD5L ELISA Kit

Target: CD5L (CD5 Molecule-Like (CD5L))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 156 pg/mL - 10000 pg/mL

Untere Nachweisgrenze: 156 pg/mL

Applikation: ELISA

Hersteller Produkt- Nr.: koa0853

Hersteller: Rockland

Verwendungszweck: Human CD5L Sandwich ELISA Kit for Quantitative Detection

Marke: AccuSignal™

Proben: Cell Culture Lysate, Cell Culture Supernatant, Plasma (EDTA - heparin), Serum, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität:

Production: Natural and recombinant human CD5L. There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: < 10 pg/mL

Bestandteile:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Anwendungsinformationen

Applikationshinweise:

ELISA_Dilution: 156pg/mL-10,000pg/mL

Application_Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 313pg/mL, 156pg/mL human CD5L standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma (heparin, EDTA) to each empty well. It is recommended that each human CD5L standard solution and each sample be measured in duplicate.

Kommentare:

Standard: Expression system for standard: NSO, Immunogen sequence: S20-G347

Probenmenge: 100 μL

Plattentyp: Pre-coated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Haltbarkeit: 12 months

Details zu CD5L

Target: CD5L (CD5 Molecule-Like (CD5L))

Andere Bezeichnung: CD5L (CD5L Produkte)

Hintergrund:

Synonyms: AAC-11, AIM, API6, APOPTOSIS INHIBITOR 6, APOPTOSIS INHIBITOR OF MACROPHAGES, CD5 antigen like (scavenger receptor cysteine rich family), CD5 antigen-like, Cd5l, CD5L_HUMAN, CT 2, CT-2, Highly similar to ANTIGEN WC1.1 [Bos taurus], IgM associated peptide, IgM-associated peptide, Pdp, PRO229, SCAVENGER RECEPTOR CYSTEINE-RICH FAMILY, SP ALPHA, SP-alpha, Spalpha

Background: CD5 antigen-like, also known as Sp alpha and AIM, is a protein that in humans is encoded by the CD5L gene. It is mapped to 1q21-q23 by fluorescence in situ hybridization. It is found that Aim expression is induced in mouse macrophages in response to loading with highly oxidized low density lipoprotein (oxLDL), and that Aim is expressed in foam cells within atherosclerotic lesions. Both the expression of Aim in lesions and its induction by oxLDL require Lxr /Rxr heterodimers. Aim-null macrophages are highly susceptible to oxLDL-induced apoptosis in vitro and undergo accelerated apoptosis in atherosclerotic lesions in vivo. Double knockout of Aim and Ldlr reduce atherosclerotic lesions. Therefore, it is concluded that AIM expression protects macrophages from apoptosis within atherosclerotic lesions, promoting early lesion development.

Gen-ID: 922

NCBI Accession: NP_005885

UniProt: O43866