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Epiregulin ELISA Kit

Rockland koa0840 EREG Reaktivität: Human Colorimetric Sandwich ELISA 156 pg/mL - 10000 pg/mL Cell Culture Lysate, Cell Culture Supernatant, Plasma (EDTA - heparin), Serum, Tissue Homogenate
Rockland
Produktnummer ABIN6719892
Hersteller Produkt- Nr.: koa0840
  • Target Alle Epiregulin (EREG) ELISA Kits anzeigen
    Epiregulin (EREG)
    Reaktivität
    • 11
    • 9
    • 6
    • 1
    • 1
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    156 pg/mL - 10000 pg/mL
    Untere Nachweisgrenze
    156 pg/mL
    Applikation
    ELISA
    Hersteller Produkt- Nr.
    koa0840
    Hersteller
    Rockland
    Verwendungszweck
    Human Epiregulin Sandwich ELISA Kit for Quantitative Detection
    Marke
    AccuSignal™
    Proben
    Cell Culture Lysate, Cell Culture Supernatant, Plasma (EDTA - heparin), Serum, Tissue Homogenate
    Analytische Methode
    Quantitative
    Spezifität

    Production: Natural and recombinant human Epiregulin. There is no detectable cross-reactivity with other relevant proteins.

    Sensitivität
    < 10 pg/mL
    Bestandteile
    • Antibody-coated 96-well plate
    • Target Protein Standard
    • Detection antibody
    • Detection reagent
    • Diluent buffers
    • Wash buffers
    • Substrate Solution
    • Stop solutions
    • Adhesive covers
    Top Product
    Discover our top product EREG ELISA Kit
  • Applikationshinweise

    ELISA_Dilution: 156pg/mL-10,000pg/mL

    Application_Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human Epiregulin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin , EDTA) to each empty well. It is recommended that each human Epiregulin standard solution and each sample be measured in duplicate.

    Kommentare

    Standard: Expression system for standard: E.coli, Immunogen sequence: V60-L108

    Probenmenge
    100 μL
    Plattentyp
    Pre-coated
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
    Haltbarkeit
    12 months
  • Target Alle Epiregulin (EREG) ELISA Kits anzeigen
    Epiregulin (EREG)
    Andere Bezeichnung
    Epiregulin (EREG Produkte)
    Hintergrund

    Synonyms: Epiregulin, EPR, ER, Ereg, EREG_HUMAN, Proepiregulin

    Background: Epiregulin (EPR) is a protein that in humans is encoded by the EREG gene. It is a member of the epidermal growth factor family. Epiregulin can function as a ligand of epidermal growth factor receptor (EGFR), as well as a ligand of most members of the ERBB (v-erb-b2 oncogene homolog) family of tyrosine-kinase receptors. The secondary structure at the C-terminus epiregulin is different from other epidermal growth factor family ligands because of the lack of hydrogen bonds. The structural difference at the C-terminus may provide an explanation for the reduced binding affinity of epiregulin to the ERBB receptors.

    Gen-ID
    2069
    NCBI Accession
    NP_001423
    UniProt
    O14944
    Pathways
    RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Regulation of Muscle Cell Differentiation
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