ERBB3 ELISA Kit

Produktnummer ABIN6719893

Produktdetails ERBB3 ELISA Kit

Target: ERBB3 (Receptor Tyrosine-Protein Kinase ErbB-3 (ERBB3))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 156 pg/mL - 10000 pg/mL

Untere Nachweisgrenze: 156 pg/mL

Applikation: ELISA

Verwendungszweck: Sandwich ELISA for Quantitative Detection of Antigen

Proben: Cell Culture Lysate, Cell Culture Supernatant, Plasma (EDTA - heparin), Serum, Tissue Homogenate

Analytische Methode: Quantitative

Produktmerkmale:

Synonyms: c erbB3, Erb b2 receptor tyrosine kinase 3, ERBB3 protein, erbB3 S, ERBB3_HUMAN, Glial growth factor receptor, HER 3, R3, Human epidermal growth factor receptor 3, LCCS2, MDA BF 1, p180 ErbB3, p45 sErbB3, p85 sErbB3, proto-oncogene-like protein c ErbB3, Receptor tyrosine protein kinase erbB 3, Tyrosine kinase type cell surface receptor HER3, Tyrosine kinase-type cell surface receptor HER3, v erb b2 avian erythroblastic leukemia viral oncogene homolog 3, v erb b2 erythroblastic leukemia viral oncogene homolog 3

Background: Receptor tyrosine-protein kinase erbB-3, also known as HER3 (human epidermal growth factor receptor 3), is a membrane bound protein that in humans is encoded by the ERBB3 gene. ErbB3 has been shown to bind the ligands heregulin and NRG-2. Ligand binding causes a change in conformation that allows for dimerization, phosphorylation, and activation of signal transduction. ErbB3 can heterodimerize with any of the other three ErbB family members. The theoretical ErbB3 homodimer would be non-functional because the kinase-impaired protein requires transphosporylation by its binding partner to be active. Unlike the other ErbB receptor tyrosine kinase family members which are activated through autophosphorylation upon ligand binding, ErbB3 is found to be kinase impaired, having only 1/1000th the autophosphorylation activity of EGFR and no ability to phosphorylate other proteins. Therefore, ErbB3 must act as anallosteric activator.

Gene Name: ERBB3

Production: Natural and recombinant human ERBB3. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: NSO, Immunogen sequence: S20-T643

Anwendungsinformationen

Applikationshinweise:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human ERBB3 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human ERBB3 standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 156pg/mL-10,000pg/mL

Probenmenge: 100 μL

Plattentyp: Pre-coated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: RT,4 °C,-20 °C

Informationen zur Lagerung: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Details zu ERBB3

Target: ERBB3 (Receptor Tyrosine-Protein Kinase ErbB-3 (ERBB3))

Andere Bezeichnung: ERBB3 - Her3 (ERBB3 Produkte)

Synonyme: ErbB-3 ELISA Kit, HER3 ELISA Kit, LCCS2 ELISA Kit, MDA-BF-1 ELISA Kit, c-erbB-3 ELISA Kit, c-erbB3 ELISA Kit, erbB3-S ELISA Kit, p180-ErbB3 ELISA Kit, p45-sErbB3 ELISA Kit, p85-sErbB3 ELISA Kit, ERBB3 ELISA Kit, C76256 ELISA Kit, Erbb-3 ELISA Kit, Erbb3r ELISA Kit, Her3 ELISA Kit, nuc-ErbB3 ELISA Kit, erb-b2 receptor tyrosine kinase 3 ELISA Kit, v-erb-b2 erythroblastic leukemia viral oncogene homolog 3 (avian) ELISA Kit, ERBB3 ELISA Kit, Erbb3 ELISA Kit

Gen-ID: 2065

NCBI Accession: NP_001005915

UniProt: P21860

Pathways: RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung