XCL1 ELISA Kit

Produktnummer ABIN6719911

Hersteller Produkt- Nr.: koa0474

Produktdetails XCL1 ELISA Kit

Target: XCL1 (Chemokine (C Motif) Ligand 1 (XCL1))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 62.5 pg/mL - 4000 pg/mL

Untere Nachweisgrenze: 62.5 pg/mL

Applikation: ELISA

Hersteller Produkt- Nr.: koa0474

Hersteller: Rockland

Verwendungszweck: Human XCL1 - Lymphotactin Sandwich ELISA Kit for Quantitative Detection

Marke: AccuSignal™

Proben: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytische Methode: Quantitative

Spezifität:

Production: Natural and recombinant human XCL1. There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: < 1 pg/mL

Bestandteile:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Anwendungsinformationen

Applikationshinweise:

ELISA_Dilution: 62.5pg/mL-4000pg/mL

Application_Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human XCL1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma (heparin, EDTA) to each empty well. It is recommended that each human XCL1 standard solution and each sample be measured in duplicate.

Kommentare:

Standard: Expression system for standard: E.coli, Immunogen sequence: V22-G114

Probenmenge: 100 μL

Plattentyp: Pre-coated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Haltbarkeit: 12 months

Details zu XCL1

Target: XCL1 (Chemokine (C Motif) Ligand 1 (XCL1))

Andere Bezeichnung: XCL1/Lymphotactin (XCL1 Produkte)

Hintergrund:

Synonyms: ATAC, C motif chemokine 1, Chemokine (C motif) ligand 1, Cytokine SCM-1, Cytokine SCM1, LPTN, LTN, Lymphotactin, Lymphotaxin, SCM 1, SCM 1 alpha, SCM-1-alpha, SCM1, SCM1 alpha, SCYC1, Small inducible cytokine C1, Small inducible cytokine subfamily C member 1, Small-inducible cytokine C1, XC chemokine ligand 1, Xcl1, XCL1_HUMAN

Background: Chemokine(C motif) ligand(XCL1) is a small cytokine belonging to the XC chemokine family that is also known as lymphotactin. This gene is mapped to chromosome 1q23 by FISH. The sequence of the deduced 114-amino acid protein is most homologous to the CC chemokines CCL8 and CCL3. It is found in high levels in spleen, thymus, intestine and peripheral blood leukocytes, and at lower levels in lung, prostate gland and ovary. XCL1 induces the migration of cells expressing XCR1.

Gen-ID: 6375

NCBI Accession: NP_002986

UniProt: P47992

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation