IL-3 ELISA Kit

Produktnummer ABIN6719916

Produktdetails IL-3 ELISA Kit

Target: IL-3 (Interleukin 3 (IL-3))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 15.6 pg/mL - 1000 pg/mL

Untere Nachweisgrenze: 15.6 pg/mL

Applikation: ELISA

Verwendungszweck: Sandwich ELISA for Quantitative Detection of Antigen

Proben: Cell Culture Supernatant, Plasma (EDTA - heparin - citrate), Serum

Analytische Methode: Quantitative

Produktmerkmale:

Synonyms: Burst promoting activity, Colony stimulating factor multiple, Eosinophil colony stimulating factor, Hematopoietic growth factor, IL 3, IL-3, IL3, IL3_HUMAN, Interleukin 3, Interleukin-3, Mast cell growth factor, MCGF, Multi CSF, Multilineage colony stimulating factor Multipotential colony stimulating factor, Multipotential colony-stimulating factor, P cell stimulating factor, P-cell-stimulating factor, Thy1 inducing factor

Background: The gene IL-3 encodes interleukin 3, a hematopoietic colony-stimulating factor(CSF) that is capable of supporting the proliferation of a broad range of hematopoietic cell types. Interleukin-3(IL-3), a protein of 140 amino acids, is chemically synthesized by means of an automated peptide synthesizer and is shown to have the biological activities attributed to native IL-3. The cDNA sequence for murine interleukin-3, one of the colony stimulating factors that regulate haematopoiesis, codes for a polypeptide of 166 amino acids including a putative signal peptide. The mouse IL 3 gene is located on chromosome 11. The human gene encoding IL 3 is tandemly arrayed on the long arm of chromosome 5. The standard product used in this kit is recombinant human IL-3, consisting of 133 amino acids with the molecular mass of 15KDa.

Gene Name: IL3

Production: Natural and recombinant human IL-3. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: E.coli, Immunogen sequence: A20-F152

Anwendungsinformationen

Applikationshinweise:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL human IL-3 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma( heparin, EDTA, citrate) to each empty well. It is recommended that each human IL-3 standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 15.6pg/mL-1000pg/mL

Probenmenge: 100 μL

Plattentyp: Pre-coated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: RT,4 °C,-20 °C

Informationen zur Lagerung: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Details zu IL-3

Target: IL-3 (Interleukin 3 (IL-3))

Andere Bezeichnung: IL-3 (IL-3 Produkte)

Synonyme: BPA ELISA Kit, Csfmu ELISA Kit, HCGF ELISA Kit, Il-3 ELISA Kit, MCGF ELISA Kit, PSF ELISA Kit, IL-3 ELISA Kit, MULTI-CSF ELISA Kit, interleukin 3 ELISA Kit, interleukin 3 (colony-stimulating factor, multiple) ELISA Kit, Il3 ELISA Kit, IL3 ELISA Kit

Gen-ID: 3562

NCBI Accession: NP_000579

UniProt: P08700

Pathways: JAK-STAT Signalweg, Regulation of Carbohydrate Metabolic Process, Autophagie