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CD13 ELISA Kit

Rockland koa0715 ANPEP Reaktivität: Maus Colorimetric Sandwich ELISA 312 pg/mL - 20000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
Rockland
Produktnummer ABIN6719920
Hersteller Produkt- Nr.: koa0715
  • Target Alle CD13 (ANPEP) ELISA Kits anzeigen
    CD13 (ANPEP) (Alanyl (Membrane) Aminopeptidase (ANPEP))
    Reaktivität
    • 6
    • 4
    • 1
    • 1
    • 1
    • 1
    • 1
    Maus
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    312 pg/mL - 20000 pg/mL
    Untere Nachweisgrenze
    312 pg/mL
    Applikation
    ELISA
    Hersteller Produkt- Nr.
    koa0715
    Hersteller
    Rockland
    Verwendungszweck
    Mouse CD13 - Aminopeptidase N Sandwich ELISA Kit for Quantitative Detection
    Marke
    AccuSignal™
    Proben
    Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
    Analytische Methode
    Quantitative
    Spezifität

    Production: Natural and recombinant mouse CD13. There is no detectable cross-reactivity with other relevant proteins.

    Sensitivität
    < 10 pg/mL
    Bestandteile
    • Antibody-coated 96-well plate
    • Target Protein Standard
    • Detection antibody
    • Detection reagent
    • Diluent buffers
    • Wash buffers
    • Substrate Solution
    • Stop solutions
    • Adhesive covers
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  • Applikationshinweise

    ELISA_Dilution: 312pg/mL-20,000pg/mL

    Application_Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 20,000pg/mL, 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL mouse CD13 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each mouse CD13 standard solution and each sample be measured in duplicate.

    Kommentare

    Standard: Expression system for standard: NSO, Immunogen sequence: K69-S966

    Probenmenge
    100 μL
    Plattentyp
    Pre-coated
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
    Haltbarkeit
    12 months
  • Target Alle CD13 (ANPEP) ELISA Kits anzeigen
    CD13 (ANPEP) (Alanyl (Membrane) Aminopeptidase (ANPEP))
    Andere Bezeichnung
    CD13/Aminopeptidase N (ANPEP Produkte)
    Hintergrund

    Synonyms: Alanyl (membrane) aminopeptidase, Alanyl aminopeptidase, Alanyl membrane aminopeptidase, Aminopeptidase M, Aminopeptidase N, AMPN_HUMAN, ANPEP, AP M, AP N, AP-M, AP-N, APN, CD 13, CD13, CD13 antigen, gp150, hAPN, Lap 1, Lap1, Microsomal aminopeptidase, Myeloid plasma membrane glycoprotein CD13, p150, PEPN

    Background: Alanine aminopeptidase, also known as ANPEP or CD13. is an enzyme that is used as a biomarker to detect damage to the kidneys, and that may be used to help diagnose certain kidney disorders. It is mapped to 15q26.1. Aminopeptidase N is located in the small-intestinal and renal microvillar membrane, and also in other plasma membranes. In the small intestine, Aminopeptidase N plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases, and it is also thought to be involved in the metabolism of regulatory peptides by diverse cell types, including small intestinal and renal tubular epithelial cells, macrophages, granulocytes, and synaptic membranes from the CNS. Petrovic et al showed that CD13 was required for endothelial cell invasion in response to bradykinin. Inhibition of CD13 abrogated internalization of bradykinin receptor B2 and reduced endothelial cell motility.

    Gen-ID
    16790
    NCBI Accession
    NP_032512
    UniProt
    P97449
    Pathways
    Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones
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