CD166 ELISA Kit

Produktnummer ABIN6719924

Produktdetails CD166 ELISA Kit

Target: CD166 (ALCAM) (Activated Leukocyte Cell Adhesion Molecule (ALCAM))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 312 pg/mL - 20000 pg/mL

Untere Nachweisgrenze: 312 pg/mL

Applikation: ELISA

Verwendungszweck: Sandwich ELISA for Quantitative Detection of Antigen

Proben: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytische Methode: Quantitative

Produktmerkmale:

Synonyms: Activated leukocyte cell adhesion molecule, ALCAM, ALCAM protein, CD 166, CD166, CD166 antigen, CD166_HUMAN, FLJ3851, FLJ38514, MEMD, MGC71733

Background: CD166 antigen is a 100-105 kD typeI transmembrane glycoprotein that is a member of the immunoglobulin superfamily of proteins. In humans it is encoded by the ALCAM gene. The gene was mapped to human chromosome 3q13.1-q13.2 by fluorescence in situ hybridization of cDNA probes to metaphase chromosomes. It is also called CD166(cluster of differentiation 166), MEMD, SC-1/DM-GRASP/BEN in the chicken, and KG-CAM in the rat. It is expressed on activated T cells, activated monocytes, epithelial cells, fibroblasts, neurons, melanoma cells, and also in sweat and sebaceous glands. CD166 protein expression is reported to be upregulated in a cell line deriving from a metastasizing melanoma. CD166 plays an important role in mediating adhesion interactions between thymic epithelial cells and CD6+ cells during intrathymic T cell development. Recently, CD166 has also been used as a potential cancer stem cell marker.

Gene Name: ALCAM

Production: Natural and recombinant human ALCAM. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: NSO, Immunogen sequence: W28-A526

Anwendungsinformationen

Applikationshinweise:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 20000pg/mL, 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL human ALCAM standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma (heparin, EDTA) to each empty well. We recommend that each human ALCAM standard solution and each sample is measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 312pg/mL-20000pg/mL

Probenmenge: 100 μL

Plattentyp: Pre-coated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: RT,4 °C,-20 °C

Informationen zur Lagerung: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Details zu CD166

Target: CD166 (ALCAM) (Activated Leukocyte Cell Adhesion Molecule (ALCAM))

Andere Bezeichnung: ALCAM (ALCAM Produkte)

Synonyme: CD166 ELISA Kit, MEMD ELISA Kit, ALCAM ELISA Kit, BEN ELISA Kit, DM-GRASP ELISA Kit, JC7 ELISA Kit, SC1 ELISA Kit, AI853494 ELISA Kit, MuSC ELISA Kit, alcam ELISA Kit, cd166 ELISA Kit, memd ELISA Kit, fb53h09 ELISA Kit, fb80a10 ELISA Kit, nlcam ELISA Kit, si:ch211-190h2.4 ELISA Kit, wu:fb53h09 ELISA Kit, wu:fb80a10 ELISA Kit, wu:fc14b01 ELISA Kit, activated leukocyte cell adhesion molecule ELISA Kit, activated leukocyte cell adhesion molecule a ELISA Kit, activated leukocyte cell adhesion molecule L homeolog ELISA Kit, CD166 antigen homolog ELISA Kit, activated leukocyte cell adhesion molecule b ELISA Kit, ALCAM ELISA Kit, Alcam ELISA Kit, alcama ELISA Kit, alcam.L ELISA Kit, alcam ELISA Kit, cd166 ELISA Kit, alcamb ELISA Kit

Gen-ID: 214

NCBI Accession: NP_001230209

UniProt: Q13740