sL-Selectin ELISA Kit

Produktnummer ABIN6719972

Produktdetails sL-Selectin ELISA Kit

Target: sL-Selectin (Selectin L (Soluble) (sL-Selectin))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 62.5 pg/mL - 4000 pg/mL

Untere Nachweisgrenze: 62.5 pg/mL

Applikation: ELISA

Verwendungszweck: Sandwich ELISA for Quantitative Detection of Antigen

Proben: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytische Methode: Quantitative

Produktmerkmale:

Synonyms: CD62 antigen ligand, CD62L antigen, IgA nephropathy, L Selectin, L selectin precursor, LAM1, LECAM1, Leu8, Leukocyte adhesion molecule 1, Leukocyte endothelial cell adhesion molecule 1, Leukocyte surface antigen Leu8, Leukocyte-endothelial cell adhesion molecule 1, LNHR, LSEL, Ly-22, Ly-m22, Lyam 1, LYAM1, Lymph node homing receptor, Lymphocyte adhesion molecule 1, Lymphocyte antigen 22, Lymphocyte surface MEL-14 antigen, PLNHR, Selectin L, Selectin, lymphocyte

Background: L-selectin, also known as CD62L, is a cell adhesion molecule found on leukocytes. It belongs to the selectin family of proteins, which recognize sialylated carbohydrate groups. It is cleaved by ADAM17.SELL(L-selectin)is a cell surface component that is a member of a family of adhesion/homing receptors which play important roles in leukocyte-endothelial cell interactions. The molecule is composed of multiple domains: one homologous to lectins, one to epidermal growth factor, and two to the consensus repeat units found in C3/C4 binding proteins.1L-selectin acts as a "homing receptor" for leukocytes to enter secondary lymphoid tissues via high endothelial venules. Ligands present on endothelial cells will bind to leukocyte expressing L-selectin, slowing leukocyte trafficking through the blood, and facilitating entry into a secondary lymphoid organ at that point2.

Gene Name: SELL

Production: Natural and recombinant human sL-Selectin. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: NSO, Immunogen sequence: W39-N332

Anwendungsinformationen

Applikationshinweise:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human sL-Selectin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA, citrate) to each empty well. It is recommended that each human sL-Selectin standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 62.5pg/mL-4000pg/mL

Probenmenge: 100 μL

Plattentyp: Pre-coated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: RT,4 °C,-20 °C

Informationen zur Lagerung: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Details zu sL-Selectin

Target: sL-Selectin (Selectin L (Soluble) (sL-Selectin))

Andere Bezeichnung: sL-Selectin (sL-Selectin Produkte)

Gen-ID: 6402

NCBI Accession: NP_000646

UniProt: P14151