VEGFC ELISA Kit

Produktnummer ABIN6719977

Produktdetails VEGFC ELISA Kit

Target: VEGFC (Vascular Endothelial Growth Factor C (VEGFC))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 62.5 pg/mL - 4000 pg/mL

Untere Nachweisgrenze: 62.5 pg/mL

Applikation: ELISA

Verwendungszweck: Sandwich ELISA for Quantitative Detection of Antigen

Proben: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytische Methode: Quantitative

Produktmerkmale:

Synonyms: Flt4 L, Flt4 ligand, FLT4 ligand DHM, Vascular endothelial growth factor C, Vascular endothelial growth factor related protein, VEGF C, VRP

Background: Vascular endothelial growth factor C is a VEGF. The human gene encoding it is VEGFC. The protein encoded by this gene is a member of the platelet-derived growth factor/vascular endothelial growth factor(PDGF/VEGF) family, is active in angiogenesis, lymphangiogenesis and endothelial cell growth and survival, and can also affect the permeability of blood vessels. This secreted protein undergoes a complex proteolytic maturation, generating multiple processed forms which bind and activate VEGFR-3 receptors. Only the fully processed form can bind and activate VEGFR-2 receptors. This protein is structurally and functionally similar to vascular endothelial growth factor D(VEGF-D). The C terminus of VEGFC has cysteine-rich repeat units characteristic of the Balbiani ring 3 protein(BR3P) of the midge Chironomus tentans.1,2 The standard product used in this kit is recombinant human VEGF-C, consisting of 135 amino acids with the molecular mass of 23Kda after glycosylation.

Gene Name: VEGFC

Production: Natural human VEGF-C, recombinant human VEGF-C (wild type), and recombinant human VEGF-C (Cys156Ser substituted). There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: NSO, Immunogen sequence: T103-R227

Anwendungsinformationen

Applikationshinweise:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 4000pg/mL,2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human VEGF-C standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human VEGF-C standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 62.5pg/mL-4000pg/mL

Probenmenge: 100 μL

Plattentyp: Pre-coated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: RT,4 °C,-20 °C

Informationen zur Lagerung: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Details zu VEGFC

Target: VEGFC (Vascular Endothelial Growth Factor C (VEGFC))

Andere Bezeichnung: VEGF-C (VEGFC Produkte)

Synonyme: VEGFC ELISA Kit, vegf-c ELISA Kit, xvegf-c ELISA Kit, Flt4-L ELISA Kit, VRP ELISA Kit, AW228853 ELISA Kit, VEGF-C ELISA Kit, vascular endothelial growth factor c ELISA Kit, vascular endothelial growth factor C ELISA Kit, vascular endothelial growth factor C S homeolog ELISA Kit, vegfc ELISA Kit, VEGFC ELISA Kit, vegfc.S ELISA Kit, Vegfc ELISA Kit

Gen-ID: 7424

NCBI Accession: NP_005420

UniProt: P49767

Pathways: RTK Signalweg, Signaling Events mediated by VEGFR1 and VEGFR2