CCL3 ELISA Kit

Produktnummer ABIN6720004

Hersteller Produkt- Nr.: koa0258

Produktdetails CCL3 ELISA Kit

Target: CCL3 (Chemokine (C-C Motif) Ligand 3 (CCL3))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 7.8 pg/mL - 500 pg/mL

Untere Nachweisgrenze: 7.8 pg/mL

Applikation: ELISA

Hersteller Produkt- Nr.: koa0258

Hersteller: Rockland

Verwendungszweck: Mouse CCL3 - MIP1 alpha Sandwich ELISA Kit for Quantitative Detection

Marke: AccuSignal™

Proben: Cell Culture Supernatant, Plasma (EDTA - heparin - citrate), Serum

Analytische Methode: Quantitative

Spezifität:

Production: Natural and recombinant mouse CCL3. There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: < 10 pg/mL

Bestandteile:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Anwendungsinformationen

Applikationshinweise: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.3pg/mL, 15.6pg/mL, 7.8pg/mL mouse CCL3 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA, citrate) to each empty well. We recommend that each mouse CCL3 standard solution and each sample is measured in duplicate.

Kommentare:

Standard: Expression system for standard: E.coli, Immunogen sequence:  A24-A92

Probenmenge: 100 μL

Plattentyp: Pre-coated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Haltbarkeit: 12 months

Referenzen für CCL3 ELISA Kit (ABIN6720004)

Wang, Huang, Lu, Liu, Mo: "Immuno-protective vesicle-crosslinked hydrogel for allogenic transplantation." in: Nature communications, Vol. 15, Issue 1, pp. 5176, (2024) (PubMed).

Nian, Li, Ma, Li, Li, Zhang, Ohiolei, Li, Dai, Liu, Yan, Fu, Xiao, Jia: "Understanding pathogen-host interplay by expression profiles of lncRNA and mRNA in the liver of Echinococcus multilocularis-infected mice." in: PLoS neglected tropical diseases, Vol. 16, Issue 5, pp. e0010435, (2022) (PubMed).

Details zu CCL3

Target: CCL3 (Chemokine (C-C Motif) Ligand 3 (CCL3))

Andere Bezeichnung: CCL3/MIP1 alpha (CCL3 Produkte)

Hintergrund:

Synonyms: C C motif chemokine 3, CCL3, CCL3_HUMAN, Chemokine ligand 3, Chemokine ligand 3, G0/G1 switch regulatory protein 19 1, G0/G1 switch regulatory protein 19-1, G0S19 1, Heparin binding chemotaxis protein, LD78-alpha, Macrophage inflammatory protein 1 alpha, Macrophage inflammatory protein 1-alpha, MIP 1 alpha, MIP-1-alpha, MIP-1-alpha, MIP1A, PAT 464.1, SCYA 3, SCYA3, SIS alpha, SIS beta, SIS-beta, Small inducible cytokine A3, Tonsillar lymphocyte LD78 alpha protein

Background: Chemokine(C-C motif) ligand 3(CCL3), so known as Macrophage inflammatory protein-1alpha(MIP-1 alpha), is a protein that in humans is encoded by the CCL3 gene. It is a cytokine which belongs to the CC chemokine family that is involved in the acute inflammatory state in the recruitment and activation of polymorphonuclear leukocytes. This gene is mapped to 17q12. CCL3 plays a role in inflammatory responses through binding to the receptors CCR1, CCR4 and CCR5. It has been found that the activation of CCR5 by CCL3 directly and independently activates a G-protein signaling pathway through GNAI2 and a tyrosine phosphorylation signaling pathway through JAK2.

Gen-ID: 20302

NCBI Accession: NP_035467

UniProt: P10855

Pathways: Cellular Response to Molecule of Bacterial Origin, Autophagie