SFTPD ELISA Kit

Produktnummer ABIN6720007

Hersteller Produkt- Nr.: koa0685

Produktdetails SFTPD ELISA Kit

Target: SFTPD (Surfactant Protein D (SFTPD))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 312 pg/mL - 20000 pg/mL

Untere Nachweisgrenze: 312 pg/mL

Applikation: ELISA

Hersteller Produkt- Nr.: koa0685

Hersteller: Rockland

Verwendungszweck: Mouse SP-D Sandwich ELISA Kit for Quantitative Detection

Marke: AccuSignal™

Proben: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytische Methode: Quantitative

Spezifität:

Production: Natural and recombinant mouse SP-D. There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: < 10 pg/mL

Bestandteile:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Anwendungsinformationen

Applikationshinweise: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 20,000pg/mL, 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL mouse SP-D standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each mouse SP-D standard solution and each sample be measured in duplicate.

Kommentare:

Standard: Expression system for standard: CHO, Immunogen sequence: A20-F374

Probenmenge: 100 μL

Plattentyp: Pre-coated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Haltbarkeit: 12 months

Details zu SFTPD

Target: SFTPD (Surfactant Protein D (SFTPD))

Andere Bezeichnung: SP-D (SFTPD Produkte)

Hintergrund:

Synonyms: COLEC 7, COLEC7, Collectin-7, Collectin7, Lung surfactant protein D, PSP D, PSP-D, PSP-D Surfactant protein D, PSPD, Pulmonary surfactant apoprotein, Pulmonary surfactant associated protein D, Pulmonary surfactant associated protein PSP-D, Pulmonary surfactant-associated protein D, SFTP 4, SFTP4, SFTPD, SFTPD_HUMAN, SP D, SP-D, Surfactant associated protein pulmonary 4, Surfactant pulmonary associated protein D

Background: Surfactant, pulmonary-associated protein D, also known as SFTPD or SP-D, is a protein which in humans is encoded by the SFTPD gene. By fluorescence in situ hybridization, the SP-D gene was localized in 10q22.2-q23.1. On the basis of homology with other collectins, potential functions for SP-D include roles in innate immunity and surfactant metabolism, SP-D is produced in the bronchiolar and terminal epithelium of human fetal lung from about 21 weeks of gestation. What's more, SP-A and SP-D act as dual-function surveillance molecules that reverse orientation and function and become initiators of host-defense reactions.

Gen-ID: 20390

NCBI Accession: NP_033186

UniProt: P50404