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Neuropilin 1 ELISA Kit

Rockland koa0817 NRP1 Reaktivität: Human Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
Rockland
Produktnummer ABIN6720012
Hersteller Produkt- Nr.: koa0817
  • Target Alle Neuropilin 1 (NRP1) ELISA Kits anzeigen
    Neuropilin 1 (NRP1)
    Reaktivität
    • 9
    • 8
    • 6
    • 1
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    0.78 ng/mL - 50 ng/mL
    Untere Nachweisgrenze
    0.78 ng/mL
    Applikation
    ELISA
    Hersteller Produkt- Nr.
    koa0817
    Hersteller
    Rockland
    Verwendungszweck
    Human Neuropilin-1 Sandwich ELISA Kit for Quantitative Detection
    Marke
    AccuSignal™
    Proben
    Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
    Analytische Methode
    Quantitative
    Spezifität

    Production: Natural and recombinant human Neuropilin-1. There is no detectable cross-reactivity with other relevant proteins.

    Sensitivität
    < 20 pg/mL
    Bestandteile
    • Antibody-coated 96-well plate
    • Target Protein Standard
    • Detection antibody
    • Detection reagent
    • Diluent buffers
    • Wash buffers
    • Substrate Solution
    • Stop solutions
    • Adhesive covers
    Top Product
    Discover our top product NRP1 ELISA Kit
  • Applikationshinweise

    ELISA_Dilution: 0.78 ng/mL-50 ng/mL

    Application_Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 50 ng/mL, 25 ng/mL, 12.5 ng/mL, 6.25 ng/mL, 3.12 ng/mL, 1.56 ng/mL, 0.78 ng/mL human Neuropilin-1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human Neuropilin-1 standard solution and each sample be measured in duplicate.

    Kommentare

    Standard: Expression system for standard: NSO, Immunogen sequence: F22-K644

    Probenmenge
    100 μL
    Plattentyp
    Pre-coated
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
    Haltbarkeit
    12 months
  • Target Alle Neuropilin 1 (NRP1) ELISA Kits anzeigen
    Neuropilin 1 (NRP1)
    Andere Bezeichnung
    Neuropilin-1 (NRP1 Produkte)
    Hintergrund

    Synonyms: A5 protein, BDCA4, BLOOD DENDRITIC CELL ANTIGEN 4, CD 304, CD304, DKFZp686A03134, DKFZp781F1414, Neuropilin-1, Neuropilin1, Neuropilin1 transmembrane receptor, NP1, NPN1, NRP 1, NRP, NRP1, NRP1_HUMAN, Vascular endothelial cell growth factor 165 receptor, VEGF165R

    Background: NRP1(Neuropilin 1) also known as NP1, NRP, BDCA4 or VEGF165R, is a membrane-bound coreceptor to a tyrosine kinase receptor for both vascular endothelial growth factor (VEGF) and semaphorin family members. NRP1 plays versatile roles in angiogenesis, axon guidance, cell survival, migration, and invasion. By somatic cell hybrid analysis, the NRP1 gene was mapped to chromosome 10. NRP1 bounds PGF1 with lower affinity. NRP1-mediated interactions are a necessary element in the initiation of the primary immune response and offer another example, like that of agrin, of a molecule shared by neurologic and immunologic synapses. After T-cell contact with DC, T-cell NRP1 colocalized with CD3 in the immunologic synapse and, sometimes, also at the opposite pole of the T cell. Soluble NRP1 interacts in a homophilic fashion with NRP1 on both DC and T cells, and this binding can be inhibited by blocking antibodies to NRP1. Furthermore, selective NRP1 inhibition in this model suppressed neovascular formation substantially.

    Gen-ID
    8829
    NCBI Accession
    NP_001019799
    UniProt
    O14786
    Pathways
    Regulation of Cell Size, Signaling Events mediated by VEGFR1 and VEGFR2, Smooth Muscle Cell Migration, Platelet-derived growth Factor Receptor Signaling, VEGFR1 Specific Signals
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