N-Cadherin ELISA Kit

Produktnummer ABIN6720019

Produktdetails N-Cadherin ELISA Kit

Target: N-Cadherin (CDH2) (Cadherin 2 (CDH2))

Reaktivität: Ratte

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.78 ng/mL - 50 ng/mL

Untere Nachweisgrenze: 0.78 ng/mL

Applikation: ELISA

Verwendungszweck: Sandwich ELISA for Quantitative Detection of Antigen

Proben: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytische Methode: Quantitative

Produktmerkmale:

Synonyms: CDw325, CDHN, Neural cadherin, N-cadherin, CD325

Background: Cadherin-2(CDH2), also known as neural cadherin(NCAD), is a protein that in humans is encoded by the CDH2 gene. It is a classical cadherin from the cadherin superfamily. This gene is mapped to 18q12.1. Cadherin-2 is expressed in the brain, skeletal and cardiac muscle. Cadherin-2 is commonly found in cancer cells and provides a mechanism for transendothelial migration. It is a calcium dependent cell-cell adhesion glycoprotein comprising five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. The protein functions during gastrulation and is required for establishment of left-right asymmetry. At certain central nervous system synapses, presynaptic to postsynaptic adhesion is mediated at least in part by this gene product.

Gene Name: CDH2

Production: Natural and recombinant rat Cadherin-2. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: NSO, Immunogen sequence: D160-A724

Anwendungsinformationen

Applikationshinweise:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 50 ng/mL, 25 ng/mL, 12.5 ng/mL, 6.25 ng/mL, 3.12 ng/mL, 1.56 ng/mL, 0.78 ng/mL rat Cadherin-2 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. We recommend that each rat Cadherin-2 standard solution and each sample is measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 0.78 ng/mL-50 ng/mL

Probenmenge: 100 μL

Plattentyp: Pre-coated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: RT,4 °C,-20 °C

Informationen zur Lagerung: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Details zu N-Cadherin

Target: N-Cadherin (CDH2) (Cadherin 2 (CDH2))

Andere Bezeichnung: Cadherin-2 - N-Cadherin (CDH2 Produkte)

Synonyme: CD325 ELISA Kit, CDHN ELISA Kit, CDw325 ELISA Kit, NCAD ELISA Kit, N-cadherin ELISA Kit, cadherin-2 ELISA Kit, cdhn ELISA Kit, ncad ELISA Kit, cdw325 ELISA Kit, CDH2 ELISA Kit, Cadherin-2 ELISA Kit, Ncad ELISA Kit, glo ELISA Kit, lyr ELISA Kit, pac ELISA Kit, wu:fb47h04 ELISA Kit, cadherin 2 ELISA Kit, cadherin 2 S homeolog ELISA Kit, cadherin 2, type 1, N-cadherin (neuronal) ELISA Kit, CDH2 ELISA Kit, cdh2.S ELISA Kit, cdh2 ELISA Kit, Cdh2 ELISA Kit

Gen-ID: 83501

NCBI Accession: NP_112623

UniProt: Q9Z1Y3

Pathways: Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Synaptic Membrane