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CD97 ELISA Kit

CD97 Reaktivität: Human Colorimetric Sandwich ELISA 125 pg/mL - 8000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
Produktnummer ABIN6720027
  • Target Alle CD97 ELISA Kits anzeigen
    CD97
    Reaktivität
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    125 pg/mL - 8000 pg/mL
    Untere Nachweisgrenze
    125 pg/mL
    Applikation
    ELISA
    Verwendungszweck
    Sandwich ELISA for Quantitative Detection of Antigen
    Proben
    Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
    Analytische Methode
    Quantitative
    Produktmerkmale

    Synonyms: CD 97, CD_antigen=CD97, CD97, CD97 antigen, CD97 antigen subunit beta, CD97 Molecule, CD97_HUMAN, Leukocyte antigen CD97, Seven span transmembrane protein, Seven transmembrane helix receptor, Seven transmembrane heterodimeric receptor associated with inflammation, TM7LN 1, TM7LN1

    Background: Cluster of differentiation 97 is a protein also known as BL-Ac [F2] encoded by the ADGRE5 gene. The CD97 gene is mapped to 19p13.2-p13.12 by fluorescence in situ hybridization. In the immune system, CD97 is known as a critical mediator of host defense. Upon lymphoid, myeloid cells and neutrophil activation, CD97 is upregulated to promote adhesion and migration to sites of inflammation. Moreover, it has been shown that CD97 regulates granulocyte homeostasis. CD97 is expressed at the sarcoplasmic reticulum and the peripheral sarcolemma in skeletal muscle. However, lack of CD97 only affects the structure of the sarcoplasmic reticulum, but not the function of skeletal muscle.

    Gene Name: ADGRE5

    Production: Natural and recombinant human CD97. There is no detectable cross-reactivity with other relevant proteins.

    Standard: Expression system for standard: NSO, Immunogen sequence: Q21-Q398

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  • Applikationshinweise

    Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 8000pg/mL, 4000pg/mL, 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL human CD97 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human CD97 standard solution and each sample be measured in duplicate.

    Blood Product Anticoagulant: Heparin Sodium

    ELISA Dilution: 125pg/mL-8000pg/mL

    Probenmenge
    100 μL
    Plattentyp
    Pre-coated
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    RT,4 °C,-20 °C
    Informationen zur Lagerung
    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
  • Target Alle CD97 ELISA Kits anzeigen
    CD97
    Andere Bezeichnung
    CD97 (CD97 Produkte)
    Synonyme
    AA409984 ELISA Kit, TM7LN1 ELISA Kit, adhesion G protein-coupled receptor E5 ELISA Kit, Adgre5 ELISA Kit, ADGRE5 ELISA Kit
    Gen-ID
    976
    NCBI Accession
    NP_001020331
    UniProt
    P48960
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