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IL15RA ELISA Kit

IL15RA Reaktivität: Maus Colorimetric Sandwich ELISA 125 pg/mL - 8000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
Produktnummer ABIN6720034
  • Target Alle IL15RA ELISA Kits anzeigen
    IL15RA (Interleukin 15 Receptor, alpha (IL15RA))
    Reaktivität
    • 5
    • 4
    Maus
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    125 pg/mL - 8000 pg/mL
    Untere Nachweisgrenze
    125 pg/mL
    Applikation
    ELISA
    Verwendungszweck
    Sandwich ELISA for Quantitative Detection of Antigen
    Proben
    Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
    Analytische Methode
    Quantitative
    Produktmerkmale

    Synonyms: AA690181, CD215, I15RA_HUMAN, IL 15R alpha, IL-15 receptor subunit alpha, IL-15R-alpha, IL-15RA, IL15RA, Interleukin 15 receptor alpha, Interleukin 15 receptor subunit alpha, MGC104179, sIL-15 receptor subunit alpha, sIL-15R-alpha, sIL-15RA, Soluble interleukin 15 receptor subunit alpha, Soluble interleukin-15 receptor subunit alpha

    Background: Interleukin 15 receptor, alpha subunit is a subunit of the interleukin 15 receptor which is encoded by the gene IL15RA in humans. The human IL15RA gene was mapped to chromosome 10p15-p14 by FISH and the mouse Il15ra gene was mapped to chromosome 2. The cytoplasmic domain of IL15R-alpha, like that of IL2R-alpha, was dispensable for mitogenic signaling, suggesting that the primary role of the alpha chains is to confer high-affinity binding. At high concentrations, IL15, like IL2, was able to signal through a complex of IL2R-beta and -gamma in the absence of the alpha subunit. The soluble receptor displayed high affinity for IL15 and inhibited both binding of IL15 to the membrane receptor and IL15-induced cell proliferation.

    Gene Name: IL15RA

    Production: Natural and recombinant mouse IL15RA. There is no detectable cross-reactivity with other relevant proteins.

    Standard: Expression system for standard: NSO, Immunogen sequence: G33-K205

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  • Applikationshinweise

    Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 8000pg/mL, 4000pg/mL, 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL mouse IL15RA standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each mouse IL15RA standard solution and each sample be measured in duplicate.

    Blood Product Anticoagulant: Heparin Sodium

    ELISA Dilution: 125pg/mL-8000pg/mL

    Probenmenge
    100 μL
    Plattentyp
    Pre-coated
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    RT,4 °C,-20 °C
    Informationen zur Lagerung
    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
  • Target Alle IL15RA ELISA Kits anzeigen
    IL15RA (Interleukin 15 Receptor, alpha (IL15RA))
    Andere Bezeichnung
    IL15RA (IL15RA Produkte)
    Synonyme
    IL15RA ELISA Kit, CD215 ELISA Kit, AA690181 ELISA Kit, IL-15RA ELISA Kit, interleukin 15 receptor subunit alpha ELISA Kit, interleukin 15 receptor, alpha chain ELISA Kit, IL15RA ELISA Kit, Il15ra ELISA Kit
    Gen-ID
    16169
    NCBI Accession
    NP_001258426
    UniProt
    Q60819
    Pathways
    JAK-STAT Signalweg
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